Morphine pretreatment protects against cerebral ischemic injury via a cPKCγ‑mediated anti‑apoptosis pathway

  • Authors:
    • Xiao-Yan Zhao
    • Jun-Fa Li
    • Tian-Zuo Li
    • Chu-Xiong Pan
    • Fu-Shan Xue
    • Gu-Yan Wang
  • View Affiliations

  • Published online on: July 15, 2021     https://doi.org/10.3892/etm.2021.10448
  • Article Number: 1016
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Abstract

It has been reported that morphine pretreatment (MP) can exert neuroprotective effects, and that protein kinase C (PKC) participates in the initiation and development of ischemic/hypoxic preconditioning in the brain. However, it remains unknown whether PKC is involved in MP‑induced neuroprotection. The aim of the present study, which included in vivo and in vitro experiments, was to determine whether the conventional γ isoform of PKC (cPKCγ) was involved in the protective effects of MP against cerebral ischemic injury. The present study included an in vivo experiment using a mouse model of middle cerebral artery occlusion and an in vitro experiment using neuroblastoma N2a cells with oxygen‑glucose deprivation (OGD). Furthermore, a cPKCγ antagonist, Go6983, was used to determine the involvement of cPKCγ in the protective effects of MP against cerebral ischemic injury. In the in vivo experiment, neurological deficits, ischemic infarct volume, neural cell damage, apoptosis and caspase‑3 activation were evaluated. In the in vitro experiment, flow cytometry was used to determine the activation of caspase‑3 in N2a cells with OGD. It was found that MP protected against cerebral ischemic injury. However, intracerebroventricular injection of the cPKCγ antagonist before MP attenuated the neuroprotective effect of MP and increased the activation of cleaved caspase‑3. These findings suggested that MP may provide protection against cerebral ischemic injury via a cPKCγ‑mediated anti‑apoptosis pathway.

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September-2021
Volume 22 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhao X, Li J, Li T, Pan C, Xue F and Wang G: Morphine pretreatment protects against cerebral ischemic injury via a cPKCγ‑mediated anti‑apoptosis pathway. Exp Ther Med 22: 1016, 2021
APA
Zhao, X., Li, J., Li, T., Pan, C., Xue, F., & Wang, G. (2021). Morphine pretreatment protects against cerebral ischemic injury via a cPKCγ‑mediated anti‑apoptosis pathway. Experimental and Therapeutic Medicine, 22, 1016. https://doi.org/10.3892/etm.2021.10448
MLA
Zhao, X., Li, J., Li, T., Pan, C., Xue, F., Wang, G."Morphine pretreatment protects against cerebral ischemic injury via a cPKCγ‑mediated anti‑apoptosis pathway". Experimental and Therapeutic Medicine 22.3 (2021): 1016.
Chicago
Zhao, X., Li, J., Li, T., Pan, C., Xue, F., Wang, G."Morphine pretreatment protects against cerebral ischemic injury via a cPKCγ‑mediated anti‑apoptosis pathway". Experimental and Therapeutic Medicine 22, no. 3 (2021): 1016. https://doi.org/10.3892/etm.2021.10448