Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2021 Volume 22 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2021 Volume 22 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions

  • Authors:
    • Lei He
    • Jinsheng Xu
    • Yaling Bai
    • Huiran Zhang
    • Wei Zhou
    • Meijuan Cheng
    • Dongxue Zhang
    • Lu Zhang
    • Shenglei Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The Fourth Hospital of Hebei Medical University, Hebei Key Laboratory of Vascular Calcification in Kidney Disease, Hebei Clinical Research Center for Chronic Kidney Disease, Shijiazhuang, Hebei 050011, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1036
    |
    Published online on: July 19, 2021
       https://doi.org/10.3892/etm.2021.10468
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Vascular calcification, such as atherosclerosis, is a serious complication of chronic kidney disease that is characterized by tunica media calcification, and has gained increasing attention from researchers. The commonly observed association between vascular calcification and osteoporosis suggests a link between bone and vascular disorders. As microRNAs (miRNAs) have a wide range of gene regulation functions, such as cell proliferation, apoptosis, stress and transdifferentiation, the current study aimed to determine whether miRNAs play a vital role in the calcification and osteoblastic differentiation of rat thoracic aorta vascular smooth muscle cells (VSMCs). Gene expression analysis was performed on seven miRNAs (miR‑29a, ‑30b, ‑103a, ‑125b, ‑133a, ‑143 and ‑211) that maybe potentially involved in the differentiation of smooth muscle cells into osteoblastic cells. The results showed that the levels of miR‑29a, ‑30b, ‑103a, ‑125b and ‑143 were markedly reduced in the VSMC calcification model, particularly miR‑103a, whereas runt‑related transcription factor 2 (RUNX2) expression was increased. Furthermore, it was found that the expression of RUNX2 was significantly decreased following the upregulation of miR‑103a, and that the expression of RUNX2 was significantly increased by downregulating miR‑103a in VSMCs. Therefore, it was concluded that miR‑103a plays a notable role in the transdifferentiation of the VSMCs in high phosphorus‑induced calcification by targeting the regulation of RUNX2, and may therefore constitute a new target for the diagnosis and treatment of vascular calcification.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Kottgen A, Russell SD, Loehr LR, Crainiceanu CM, Rosamond WD, Chang PP, Chambless LE and Coresh J: Reduced kidney function as a risk factor for incident heart failure: The atherosclerosis risk in communities (ARIC) study. J Am Soc Nephrol. 18:1307–1315. 2007.PubMed/NCBI View Article : Google Scholar

2 

Tentori F, Blayney MJ, Albert JM, Gillespie BW, Kerr PG, Bommer J, Young EW, Akizawa T, Akiba T, Pisoni RL, et al: Mortality risk for dialysis patients with different levels of serum calcium, phosphorus, and PTH: The Dialysis Outcomes and Practice Patterns Study (DOPPS). Am J Kidney Dis. 52:519–530. 2008.PubMed/NCBI View Article : Google Scholar

3 

Bolasco P: Effects of the use of non-calcium phosphate binders in the control and outcome of vascular calcifications: A review of clinical trials on CKD patients. Int J Nephrol. 2011(758450)2011.PubMed/NCBI View Article : Google Scholar

4 

Adragao T: Evaluation of vascular calcifications in CKD patients. Int J Artif Organs. 32:81–86. 2009.PubMed/NCBI View Article : Google Scholar

5 

Bai Y, Zhang J, Xu J, Cui L, Zhang H and Zhang S: Alteration of type I collagen in the radial artery of patients with end-stage renal disease. Am J Med Sci. 349:292–297. 2015.PubMed/NCBI View Article : Google Scholar

6 

Towler DA and Demer LL: Thematic series on the pathobiology of vascular calcification: An introduction. Circ Res. 108:1378–1380. 2011.PubMed/NCBI View Article : Google Scholar

7 

Nitta K and Ogawa T: Vascular calcification in end-stage renal disease patients. Contrib Nephrol. 185:156–167. 2015.PubMed/NCBI View Article : Google Scholar

8 

Klimczak D, Paczek L, Jazdzewski K and Kuch M: MicroRNAs: Powerful regulators and potential diagnostic tools in cardiovascular disease. Kardiol Pol. 73:1–6. 2015.PubMed/NCBI View Article : Google Scholar

9 

Chiang VS: Withdrawn: MicroRNAs as potential regulators of docosahexaenoic acid benefits in Alzheimer's disease. Nutr Neurosci: Mar 14, 2015 (Epub ahead of print). doi: 10.1179/1476830515Y.0000000014.

10 

Mennigen JA, Plagnes-Juan E, Figueredo-Silva CA, Seiliez I, Panserat S and Skiba-Cassy S: Acute endocrine and nutritional co-regulation of the hepatic omy-miRNA-122b and the lipogenic gene fas in rainbow trout, Oncorhynchus mykiss. Comp Biochem Physiol B Biochem Mol Biol. 169:16–24. 2014.PubMed/NCBI View Article : Google Scholar

11 

Pinto MT, Nicolete LD, Rodrigues ES, Palma PV, Orellana MD, Kashima S and Covas DT: Overexpression of hsa-miR-125b during osteoblastic differentiation does not influence levels of Runx2, osteopontin, and ALPL gene expression. Braz J Med Biol Res. 46:676–680. 2013.PubMed/NCBI View Article : Google Scholar

12 

Zhang W, Wu Y, Shiozaki Y, Sugimoto Y, Takigawa T, Tanaka M, Matsukawa A and Ozaki T: MiRNA-133a-5p inhibits the expression of osteoblast differentiation-associated markers by targeting the 3 UTR of RUNX2. DNA Cell Biol. 37:199–209. 2018.PubMed/NCBI View Article : Google Scholar

13 

Narayanan A, Srinaath N, Rohini M and Selvamurugan N: Regulation of Runx2 by MicroRNAs in osteoblast differentiation. Life Sci. 232(116676)2019.PubMed/NCBI View Article : Google Scholar

14 

Xu J, Bai Y, Jin J, Zhang J, Zhang S, Cui L and Zhang H: Magnesium modulates the expression levels of calcification-associated factors to inhibit calcification in a time-dependent manner. Exp Ther Med. 9:1028–1034. 2015.PubMed/NCBI View Article : Google Scholar

15 

Griffiths-Jones S, Grocock RJ, van Dongen S, Bateman A and Enright AJ: MiRBase: MicroRNA sequences, targets and gene nomenclature. Nucleic Acids Res. 34:D140–D144. 2006.PubMed/NCBI View Article : Google Scholar

16 

Wang Z, Jiang Y, Liu N, Ren L, Zhu Y, An Y and Chen D: Advanced glycation end-product Nε-carboxymethyl-Lysine accelerates progression of atherosclerotic calcification in diabetes. Atherosclerosis. 221:387–396. 2012.PubMed/NCBI View Article : Google Scholar

17 

Du Y, Wang Y, Wang L, Liu B, Tian Q, Liu CJ, Zhang T, Xu Q, Zhu Y, Ake O, et al: Cartilage oligomeric matrix protein inhibits vascular smooth muscle calcification by interacting with bone morphogenetic protein-2. Circ Res. 108:917–928. 2011.PubMed/NCBI View Article : Google Scholar

18 

Shanahan CM, Crouthamel MH, Kapustin A and Giachelli CM: Arterial calcification in chronic kidney disease: Key roles for calcium and phosphate. Circ Res. 109:697–711. 2011.PubMed/NCBI View Article : Google Scholar

19 

Tang Q, Tong M, Zheng G, Shen L, Shang P and Liu H: Masquelet's induced membrane promotes the osteogenic differentiation of bone marrow mesenchymal stem cells by activating the Smad and MAPK pathways. Am J Transl Res. 10:1211–1219. 2018.PubMed/NCBI

20 

Luo Y, Cao X, Chen J, Gu J, Zhao J and Sun J: MicroRNA-224 suppresses osteoblast differentiation by inhibiting SMAD4. J Cell Physiol. 233:6929–6937. 2018.PubMed/NCBI View Article : Google Scholar

21 

Liu J, Xiao X, Shen Y, Chen L, Xu C, Zhao H, Wu Y, Zhang Q, Zhong J, Tang Z, et al: MicroRNA-32 promotes calcification in vascular smooth muscle cells: Implications as a novel marker for coronary artery calcification. PLoS One. 12(e0174138)2017.PubMed/NCBI View Article : Google Scholar

22 

Wang Y, Chen S, Deng C, Li F, Wang Y, Hu X, Shi F and Dong N: MicroRNA-204 Targets Runx2 to Attenuate BMP-2-induced osteoblast differentiation of human aortic valve interstitial cells. J Cardiovasc Pharmacol. 66:63–71. 2015.PubMed/NCBI View Article : Google Scholar

23 

Roberto VP, Tiago DM, Silva IA and Cancela ML: MiR-29a is an enhancer of mineral deposition in bone-derived systems. Arch Biochem Biophys. 564:173–183. 2014.PubMed/NCBI View Article : Google Scholar

24 

Wen P, Cao H, Fang L, Ye H, Zhou Y, Jiang L, Su W, Xu H, He W, Dai C and Yang J: MiR-125b/Ets1 axis regulates transdifferentiation and calcification of vascular smooth muscle cells in a high-phosphate environment. Exp Cell Res. 322:302–312. 2014.PubMed/NCBI View Article : Google Scholar

25 

Goettsch C, Rauner M, Pacyna N, Hempel U, Bornstein SR and Hofbauer LC: MiR-125b regulates calcification of vascular smooth muscle cells. Am J Pathol. 179:1594–1600. 2011.PubMed/NCBI View Article : Google Scholar

26 

Balderman JA, Lee HY, Mahoney CE, Handy DE, White K, Annis S, Lebeche D, Hajjar RJ, Loscalzo J and Leopold JA: Bone morphogenetic protein-2 decreases microRNA-30b and microRNA-30c to promote vascular smooth muscle cell calcification. J Am Heart Assoc. 1(e003905)2012.PubMed/NCBI View Article : Google Scholar

27 

Sun Z, Cao X, Hu Z, Zhang L, Wang H, Zhou H, Li D, Zhang S and Xie M: MiR-103 inhibits osteoblast proliferation mainly through suppressing Cav1.2 expression in simulated microgravity. Bone. 76:121–128. 2015.PubMed/NCBI View Article : Google Scholar

28 

Li E, Zhang J, Yuan T and Ma B: MiR-143 suppresses osteogenic differentiation by targeting Osterix. Mol Cell Biochem. 390:69–74. 2014.PubMed/NCBI View Article : Google Scholar

29 

Panizo S, Naves-Diaz M, Carrillo-Lopez N, Martinez-Arias L, Fernandez-Martin JL, Ruiz-Torres MP, Cannata-Andia JB and Rodriguez I: MicroRNAs 29b, 133b, and 211 regulate vascular smooth muscle calcification mediated by high phosphorus. J Am Soc Nephrol. 27:824–834. 2016.PubMed/NCBI View Article : Google Scholar

30 

Atlasi Y, Noori R, Gaspar C, Franken P, Sacchetti A, Rafati H, Mahmoudi T, Decraene C, Calin GA, Merrill BJ and Fodde R: Wnt signaling regulates the lineage differentiation potential of mouse embryonic stem cells through Tcf3 down-regulation. PLoS Genet. 9(e1003424)2013.PubMed/NCBI View Article : Google Scholar

31 

Zuo B, Zhu J, Li J, Wang C, Zhao X, Cai G, Li Z, Peng J, Wang P, Shen C, et al: MicroRNA-103a functions as a mechanosensitive microRNA to inhibit bone formation through targeting Runx2. J Bone Miner Res. 30:330–345. 2015.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
He L, Xu J, Bai Y, Zhang H, Zhou W, Cheng M, Zhang D, Zhang L and Zhang S: MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions. Exp Ther Med 22: 1036, 2021.
APA
He, L., Xu, J., Bai, Y., Zhang, H., Zhou, W., Cheng, M. ... Zhang, S. (2021). MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions. Experimental and Therapeutic Medicine, 22, 1036. https://doi.org/10.3892/etm.2021.10468
MLA
He, L., Xu, J., Bai, Y., Zhang, H., Zhou, W., Cheng, M., Zhang, D., Zhang, L., Zhang, S."MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions". Experimental and Therapeutic Medicine 22.3 (2021): 1036.
Chicago
He, L., Xu, J., Bai, Y., Zhang, H., Zhou, W., Cheng, M., Zhang, D., Zhang, L., Zhang, S."MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions". Experimental and Therapeutic Medicine 22, no. 3 (2021): 1036. https://doi.org/10.3892/etm.2021.10468
Copy and paste a formatted citation
x
Spandidos Publications style
He L, Xu J, Bai Y, Zhang H, Zhou W, Cheng M, Zhang D, Zhang L and Zhang S: MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions. Exp Ther Med 22: 1036, 2021.
APA
He, L., Xu, J., Bai, Y., Zhang, H., Zhou, W., Cheng, M. ... Zhang, S. (2021). MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions. Experimental and Therapeutic Medicine, 22, 1036. https://doi.org/10.3892/etm.2021.10468
MLA
He, L., Xu, J., Bai, Y., Zhang, H., Zhou, W., Cheng, M., Zhang, D., Zhang, L., Zhang, S."MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions". Experimental and Therapeutic Medicine 22.3 (2021): 1036.
Chicago
He, L., Xu, J., Bai, Y., Zhang, H., Zhou, W., Cheng, M., Zhang, D., Zhang, L., Zhang, S."MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions". Experimental and Therapeutic Medicine 22, no. 3 (2021): 1036. https://doi.org/10.3892/etm.2021.10468
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team