MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions

He,Lei; Xu,Jinsheng; Bai,Yaling; Zhang,Huiran; Zhou,Wei; Cheng,Meijuan; Zhang,Dongxue; Zhang,Lu; Zhang,Shenglei

doi:10.3892/etm.2021.10468

MicroRNA‑103a regulates the calcification of vascular smooth muscle cells by targeting runt‑related transcription factor 2 in high phosphorus conditions

Authors:
- Lei He
- Jinsheng Xu
- Yaling Bai
- Huiran Zhang
- Wei Zhou
- Meijuan Cheng
- Dongxue Zhang
- Lu Zhang
- Shenglei Zhang
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Affiliations: Department of Nephrology, The Fourth Hospital of Hebei Medical University, Hebei Key Laboratory of Vascular Calcification in Kidney Disease, Hebei Clinical Research Center for Chronic Kidney Disease, Shijiazhuang, Hebei 050011, P.R. China
Published online on: July 19, 2021 https://doi.org/10.3892/etm.2021.10468
Article Number: 1036
Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Vascular calcification, such as atherosclerosis, is a serious complication of chronic kidney disease that is characterized by tunica media calcification, and has gained increasing attention from researchers. The commonly observed association between vascular calcification and osteoporosis suggests a link between bone and vascular disorders. As microRNAs (miRNAs) have a wide range of gene regulation functions, such as cell proliferation, apoptosis, stress and transdifferentiation, the current study aimed to determine whether miRNAs play a vital role in the calcification and osteoblastic differentiation of rat thoracic aorta vascular smooth muscle cells (VSMCs). Gene expression analysis was performed on seven miRNAs (miR‑29a, ‑30b, ‑103a, ‑125b, ‑133a, ‑143 and ‑211) that maybe potentially involved in the differentiation of smooth muscle cells into osteoblastic cells. The results showed that the levels of miR‑29a, ‑30b, ‑103a, ‑125b and ‑143 were markedly reduced in the VSMC calcification model, particularly miR‑103a, whereas runt‑related transcription factor 2 (RUNX2) expression was increased. Furthermore, it was found that the expression of RUNX2 was significantly decreased following the upregulation of miR‑103a, and that the expression of RUNX2 was significantly increased by downregulating miR‑103a in VSMCs. Therefore, it was concluded that miR‑103a plays a notable role in the transdifferentiation of the VSMCs in high phosphorus‑induced calcification by targeting the regulation of RUNX2, and may therefore constitute a new target for the diagnosis and treatment of vascular calcification.

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