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Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis

  • Authors:
    • Wei Wang
    • Yu-Min Ma
    • Zheng-Lin Jiang
    • Zhi-Wei Gao
    • Wei-Guan Chen
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Internal Medicine, The Second People's Hospital of Nantong, Nantong, Jiangsu 226002, P.R. China, Institute of Nautical Medicine, Nantong University, Nantong, Jiangsu 226000, P.R. China, Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 290
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    Published online on: January 27, 2021
       https://doi.org/10.3892/etm.2021.9721
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Abstract

The present study aimed to explore the pathogenesis behind post‑traumatic epilepsy (PTE). In the present study, a chloride ferric injection‑induced rat PTE model was established. The expression levels of apoptosis‑antagonizing transcription factor (AATF), cleaved caspase‑3, p53, Bcl‑2 and Bax were measured by western blotting or immunofluorescence staining (IF). The expression of AATF in vivo was downregulated by microinjection of lentiviral‑mediated short‑hairpin RNA. Compared with control and sham groups, at day 5 after PTE, neuron apoptosis was significantly increased and the expression levels of AATF, p53, cleaved caspase‑3 and Bax were significantly upregulated. In addition, IF revealed co‑localization of AATF and cleaved caspase‑3 in the cortex. Additionally, AATF was expressed mainly in neurons and astrocytes. Following AATF inhibition, the expression levels of p53 and cleaved caspase‑3 were significantly reduced as compared with the control group. Taken together, these findings suggested that following PTE, AATF is involved in neuronal apoptosis and may serve as a potential target for its alleviation.
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Copy and paste a formatted citation
Spandidos Publications style
Wang W, Ma Y, Jiang Z, Gao Z and Chen W: Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis. Exp Ther Med 21: 290, 2021.
APA
Wang, W., Ma, Y., Jiang, Z., Gao, Z., & Chen, W. (2021). Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis. Experimental and Therapeutic Medicine, 21, 290. https://doi.org/10.3892/etm.2021.9721
MLA
Wang, W., Ma, Y., Jiang, Z., Gao, Z., Chen, W."Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis". Experimental and Therapeutic Medicine 21.4 (2021): 290.
Chicago
Wang, W., Ma, Y., Jiang, Z., Gao, Z., Chen, W."Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis". Experimental and Therapeutic Medicine 21, no. 4 (2021): 290. https://doi.org/10.3892/etm.2021.9721
Copy and paste a formatted citation
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Spandidos Publications style
Wang W, Ma Y, Jiang Z, Gao Z and Chen W: Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis. Exp Ther Med 21: 290, 2021.
APA
Wang, W., Ma, Y., Jiang, Z., Gao, Z., & Chen, W. (2021). Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis. Experimental and Therapeutic Medicine, 21, 290. https://doi.org/10.3892/etm.2021.9721
MLA
Wang, W., Ma, Y., Jiang, Z., Gao, Z., Chen, W."Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis". Experimental and Therapeutic Medicine 21.4 (2021): 290.
Chicago
Wang, W., Ma, Y., Jiang, Z., Gao, Z., Chen, W."Apoptosis‑antagonizing transcription factor is involved in rat post‑traumatic epilepsy pathogenesis". Experimental and Therapeutic Medicine 21, no. 4 (2021): 290. https://doi.org/10.3892/etm.2021.9721
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