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Interleukin‑1β weakens paclitaxel sensitivity through regulating autophagy in the non‑small cell lung cancer cell line A549

  • Authors:
    • Juanwen Lian
    • Tao Hua
    • Jialing Xu
    • Jie Ding
    • Zejie Liu
    • Yu Fan
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Xi'an Chest Hospital, Xi'an, Shaanxi 710100, P.R. China
    Copyright: © Lian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 293
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    Published online on: January 27, 2021
       https://doi.org/10.3892/etm.2021.9724
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Abstract

Non‑small cell lung cancer (NSCLC) poses a threat to human health and paclitaxel chemotherapy has been approved for the treatment of this type of cancer. However, resistance to treatment severely compromises the survival rate and prognosis of patients with NSCLC. The aim of the present study was to investigate the role of IL‑1β in paclitaxel sensitivity of NSCLC cells and elucidate the underlying mechanism. The expression of IL‑1β was found to be upregulated in NSCLC tissues and cells compared with healthy adjacent tissues and a normal epithelial cell line, respectively, as detected by reverse transcription‑quantitative PCR and western blot analyses. Subsequently, Cell Counting Kit‑8 assay and flow cytometry revealed that IL‑1β weakened the sensitivity of A549 cells to paclitaxel. It was subsequently demonstrated that IL‑1β induced A549 cell autophagy, while tunicamycin‑induced autophagy increased the IL‑1β expression level and weakened paclitaxel sensitivity. Thus, the results revealed that IL‑1β reduced the sensitivity to paclitaxel in A549 cells by promoting autophagy and suggested that IL‑1β may be of value for improving the therapeutic efficacy of paclitaxel chemotherapy in NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Lian J, Hua T, Xu J, Ding J, Liu Z and Fan Y: Interleukin‑1β weakens paclitaxel sensitivity through regulating autophagy in the non‑small cell lung cancer cell line A549. Exp Ther Med 21: 293, 2021.
APA
Lian, J., Hua, T., Xu, J., Ding, J., Liu, Z., & Fan, Y. (2021). Interleukin‑1β weakens paclitaxel sensitivity through regulating autophagy in the non‑small cell lung cancer cell line A549. Experimental and Therapeutic Medicine, 21, 293. https://doi.org/10.3892/etm.2021.9724
MLA
Lian, J., Hua, T., Xu, J., Ding, J., Liu, Z., Fan, Y."Interleukin‑1β weakens paclitaxel sensitivity through regulating autophagy in the non‑small cell lung cancer cell line A549". Experimental and Therapeutic Medicine 21.4 (2021): 293.
Chicago
Lian, J., Hua, T., Xu, J., Ding, J., Liu, Z., Fan, Y."Interleukin‑1β weakens paclitaxel sensitivity through regulating autophagy in the non‑small cell lung cancer cell line A549". Experimental and Therapeutic Medicine 21, no. 4 (2021): 293. https://doi.org/10.3892/etm.2021.9724
Copy and paste a formatted citation
x
Spandidos Publications style
Lian J, Hua T, Xu J, Ding J, Liu Z and Fan Y: Interleukin‑1β weakens paclitaxel sensitivity through regulating autophagy in the non‑small cell lung cancer cell line A549. Exp Ther Med 21: 293, 2021.
APA
Lian, J., Hua, T., Xu, J., Ding, J., Liu, Z., & Fan, Y. (2021). Interleukin‑1β weakens paclitaxel sensitivity through regulating autophagy in the non‑small cell lung cancer cell line A549. Experimental and Therapeutic Medicine, 21, 293. https://doi.org/10.3892/etm.2021.9724
MLA
Lian, J., Hua, T., Xu, J., Ding, J., Liu, Z., Fan, Y."Interleukin‑1β weakens paclitaxel sensitivity through regulating autophagy in the non‑small cell lung cancer cell line A549". Experimental and Therapeutic Medicine 21.4 (2021): 293.
Chicago
Lian, J., Hua, T., Xu, J., Ding, J., Liu, Z., Fan, Y."Interleukin‑1β weakens paclitaxel sensitivity through regulating autophagy in the non‑small cell lung cancer cell line A549". Experimental and Therapeutic Medicine 21, no. 4 (2021): 293. https://doi.org/10.3892/etm.2021.9724
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