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TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway

  • Authors:
    • Peng Shen
    • Tian-Yang Zhang
    • Shu-Yan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Clinical Center of Spaceport, the Northern Medical District, Chinese People's Liberation Army General Hospital, Beijing 100094, P.R. China, The 7th Medical Center, Chinese People's Liberation Army General Hospital, Beijing 100700, P.R. China, State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Clinical Research Center for Oral Diseases, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 313
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    Published online on: February 1, 2021
       https://doi.org/10.3892/etm.2021.9744
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Abstract

Tribbles pseudokinase 3 (TRIB3), a member of the tribbles‑related family, has biological roles such as by acting as an oncogene or tumor suppressor gene, in various types of cancer, including colorectal cancer, breast cancer, lung cancer and renal cell carcinoma. However, the role of TRIB3 in oral squamous cell carcinoma (OSCC) is remains unclear. The current was aimed to determine the biological function of TRIB3 in OSCC progression. TRIB3 expression was examined in OSCC surgical specimens using reverse transcription‑quantitative PCR and the role of TRIB3 in the proliferation capacities of OSCC cell lines was examined using crystal violet and MTT assays in vitro and tumorigenicity assays in vivo. The underlying mechanism by which TRIB3 exerts its function was investigated using western blotting. The results demonstrated that the mRNA and protein expression levels of TRIB3 were higher in human OSCC tissues compared with normal tissues. The role of TRIB3 in cell proliferation was also determined. TRIB3 overexpression significantly promoted OSCC cell proliferation, whereas TRIB3 knockdown inhibited OSCC cell proliferation compared with control cells. TRIB3 knockdown also suppressed tumor growth and decreased tumor volume in vivo compared with control cells. Moreover, the results suggested that TRIB3 overexpression increased the phosphorylation of protein kinase B (AKT) and mammalian target of rapamycin (mTOR), whereas TRIB3 knockdown decreased the phosphorylation of AKT and mTOR compared with control cells. To summarize, the present study indicated that TRIB3 promoted OSCC cell proliferation by activating the AKT signaling pathway; therefore, TRIB3 may serve as a potential target for the diagnosis and treatment of OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Shen P, Zhang T and Wang S: TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway. Exp Ther Med 21: 313, 2021.
APA
Shen, P., Zhang, T., & Wang, S. (2021). TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway. Experimental and Therapeutic Medicine, 21, 313. https://doi.org/10.3892/etm.2021.9744
MLA
Shen, P., Zhang, T., Wang, S."TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway". Experimental and Therapeutic Medicine 21.4 (2021): 313.
Chicago
Shen, P., Zhang, T., Wang, S."TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway". Experimental and Therapeutic Medicine 21, no. 4 (2021): 313. https://doi.org/10.3892/etm.2021.9744
Copy and paste a formatted citation
x
Spandidos Publications style
Shen P, Zhang T and Wang S: TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway. Exp Ther Med 21: 313, 2021.
APA
Shen, P., Zhang, T., & Wang, S. (2021). TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway. Experimental and Therapeutic Medicine, 21, 313. https://doi.org/10.3892/etm.2021.9744
MLA
Shen, P., Zhang, T., Wang, S."TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway". Experimental and Therapeutic Medicine 21.4 (2021): 313.
Chicago
Shen, P., Zhang, T., Wang, S."TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway". Experimental and Therapeutic Medicine 21, no. 4 (2021): 313. https://doi.org/10.3892/etm.2021.9744
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