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miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3

  • Authors:
    • Luming Liao
    • Qiaolan Yang
    • Hailiang Li
    • Rongsen Meng
    • Yuntian Li
  • View Affiliations / Copyright

    Affiliations: The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of General Surgery, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Cardiovascular Internal Medicine, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China
    Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 323
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    Published online on: February 5, 2021
       https://doi.org/10.3892/etm.2021.9754
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Abstract

Endothelial‑cell (EC) apoptosis serves a vital role in the pathogenesis of atherosclerosis. Accumulating evidence has implicated microRNA (miRNA/miR) dysregulation in EC apoptosis. Although the role of miR‑454‑3p in carcinogenesis has been well documented, its role and underlying mechanism in EC apoptosis remain unclear. In the present study, the results revealed that miR‑454‑3p expression was substantially downregulated in human aortic endothelial cells (HAECs) following oxidized low‑density lipoprotein (ox‑LDL) treatment. miR‑454‑3p suppression significantly attenuated the viability of HAECs, while miR‑454‑3p overexpression repressed ox‑LDL‑induced HAEC apoptosis. Bioinformatics analysis and luciferase reporter assays revealed that transient receptor potential canonical 3 (TRPC3), a key regulator of atherosclerosis development, was the direct target of miR‑454‑3p. Furthermore, TRPC3 overexpression abolished the anti‑apoptotic effect of miR‑454‑3p on HAECs. These results revealed a novel role of miR‑454‑3p in ox‑LDL‑induced apoptosis in HAECs.
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Copy and paste a formatted citation
Spandidos Publications style
Liao L, Yang Q, Li H, Meng R and Li Y: miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3. Exp Ther Med 21: 323, 2021.
APA
Liao, L., Yang, Q., Li, H., Meng, R., & Li, Y. (2021). miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3. Experimental and Therapeutic Medicine, 21, 323. https://doi.org/10.3892/etm.2021.9754
MLA
Liao, L., Yang, Q., Li, H., Meng, R., Li, Y."miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3". Experimental and Therapeutic Medicine 21.4 (2021): 323.
Chicago
Liao, L., Yang, Q., Li, H., Meng, R., Li, Y."miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3". Experimental and Therapeutic Medicine 21, no. 4 (2021): 323. https://doi.org/10.3892/etm.2021.9754
Copy and paste a formatted citation
x
Spandidos Publications style
Liao L, Yang Q, Li H, Meng R and Li Y: miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3. Exp Ther Med 21: 323, 2021.
APA
Liao, L., Yang, Q., Li, H., Meng, R., & Li, Y. (2021). miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3. Experimental and Therapeutic Medicine, 21, 323. https://doi.org/10.3892/etm.2021.9754
MLA
Liao, L., Yang, Q., Li, H., Meng, R., Li, Y."miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3". Experimental and Therapeutic Medicine 21.4 (2021): 323.
Chicago
Liao, L., Yang, Q., Li, H., Meng, R., Li, Y."miR‑454‑3p prevents ox‑LDL‑induced apoptosis in HAECs by targeting TRPC3". Experimental and Therapeutic Medicine 21, no. 4 (2021): 323. https://doi.org/10.3892/etm.2021.9754
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