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Article

Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 in vitro

  • Authors:
    • Zhuokai Li
    • Xiaohe Wang
    • Shuofu Liang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, P.R. China, Department of Orthopaedics, Jinan Zhangqiu District Hospital of TCM, Jinan, Shandong 250200, P.R. China, Department of Orthopaedics, Zibo Zhoucun People's Hospital, Zibo, Shandong 255300, P.R. China
  • Article Number: 325
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    Published online on: February 5, 2021
       https://doi.org/10.3892/etm.2021.9756
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Abstract

The aim of the present study was to clarify the effect of long non‑coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) on the proliferation, migration and invasion of osteosarcoma (OS) cells and to explore the potential underlying mechanisms. The expression levels of SNHG1, microRNA (miR)‑424‑5p and fibroblast growth factor 2 (FGF2) in OS tissues and cells were detected using reverse transcription‑quantitative polymerase chain reaction. OS cell proliferation, migration and invasion were analysed by MTT, wound healing and Transwell invasion assays, respectively. The targeting relationships between SNHG1 and miR‑424‑5p, as well as between miR‑424‑5p and FGF2, were confirmed using RNA‑binding protein immunoprecipitation and/or dual‑luciferase reporter gene assays. The results demonstrated that the expression levels of SNHG1 and FGF2 were upregulated, whereas the expression of miR‑424‑5p was downregulated in OS tissues and cells. The silencing of SNHG1 significantly inhibited the proliferation, migration and invasion of OS cells. Additionally, FGF2 was shown to be a target of miR‑424‑5p, which in turn, was a target of SNHG1. miR‑424‑5p silencing and FGF2 overexpression both reversed the suppressive effects of SNHG1 knockdown on the proliferation, migration and invasion of OS cells. Thus, the silencing of SNHG1 may inhibit the proliferation, migration and invasion of OS cells by regulating the miR‑424‑5p/FGF2 axis.
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1 

Mirabello L, Troisi RJ and Savage SA: International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons. Int J Cancer. 125:229–234. 2009.PubMed/NCBI View Article : Google Scholar

2 

Kumar R, Kumar M, Malhotra K and Patel S: Primary osteosarcoma in the elderly revisited: Current concepts in diagnosis and treatment. Curr Oncol Rep. 20(13)2018.PubMed/NCBI View Article : Google Scholar

3 

Harrison DJ, Geller DS, Gill JD, Lewis VO and Gorlick R: Current and future therapeutic approaches for osteosarcoma. Expert Rev Anticancer Ther. 18:39–50. 2018.PubMed/NCBI View Article : Google Scholar

4 

Shaikh AB, Li F, Li M, He B, He X, Chen G, Guo B, Li D, Jiang F, Dang L, et al: Present advances and future perspectives of molecular targeted therapy for osteosarcoma. Int J Mol Sci. 17(506)2016.PubMed/NCBI View Article : Google Scholar

5 

Zhang B and Zhang Y, Li R, Li J, Lu X and Zhang Y: The efficacy and safety comparison of first-line chemotherapeutic agents (high-dose methotrexate, doxorubicin, cisplatin, and ifosfamide) for osteosarcoma: A network meta-analysis. J Orthop Surg Res. 15(51)2020.PubMed/NCBI View Article : Google Scholar

6 

Luetke A, Meyers PA, Lewis I and Juergens H: Osteosarcoma treatment-where do we stand? A state of the art review. Cancer Treat Rev. 40:523–532. 2014.PubMed/NCBI View Article : Google Scholar

7 

Rinn JL and Chang HY: Genome regulation by long noncoding RNAs. Annu Rev Biochem. 81:145–166. 2012.PubMed/NCBI View Article : Google Scholar

8 

Zhang Q, Geng PL, Yin P, Wang XL, Jia JP and Yao J: Down-regulation of long non-coding RNA TUG1 inhibits osteosarcoma cell proliferation and promotes apoptosis. Asian Pac J Cancer Prev. 14:2311–2315. 2013.PubMed/NCBI View Article : Google Scholar

9 

Li H, Cui J, Xu B, He S, Yang H and Liu L: Long non-coding RNA XIST serves an oncogenic role in osteosarcoma by sponging miR-137. Exp Ther Med. 17:730–738. 2019.PubMed/NCBI View Article : Google Scholar

10 

Liu P, He W, Lu Y and Wang Y: Long non-coding RNA LINC00152 promotes tumorigenesis via sponging miR-193b-3p in osteosarcoma. Oncol Lett. 18:3630–3636. 2019.PubMed/NCBI View Article : Google Scholar

11 

Zhang H, Lu Y, Wang J, Zhang T, Dong C, Li X, Wang X, Ma Q, Yang T and Zhou Y: Downregulation of the long non-coding RNA FOXD2-AS1 inhibits cell proliferation, migration and invasion in osteosarcoma. Mol Med Rep. 20:292–302. 2019.PubMed/NCBI View Article : Google Scholar

12 

Deng R, Zhang J and Chen J: lncRNA SNHG1 negatively regulates miRNA-101-3p to enhance the expression of ROCK1 and promote cell proliferation, migration and invasion in osteosarcoma. Int J Mol Med. 43:1157–1166. 2019.PubMed/NCBI View Article : Google Scholar

13 

Jiang Z, Jiang C and Fang J: Up-regulated lnc-SNHG1 contributes to osteosarcoma progression through sequestration of miR-577 and activation of WNT2B/Wnt/β-catenin pathway. Biochem Biophys Res Commun. 495:238–245. 2018.PubMed/NCBI View Article : Google Scholar

14 

Wang J, Cao L, Wu J and Wang Q: Long non-coding RNA SNHG1 regulates NOB1 expression by sponging miR-326 and promotes tumorigenesis in osteosarcoma. Int J Oncol. 52:77–88. 2018.PubMed/NCBI View Article : Google Scholar

15 

Lu TX and Rothenberg ME: MicroRNA. J Allergy Clin Immunol. 141:1202–1207. 2018.PubMed/NCBI View Article : Google Scholar

16 

Fei D, Sui G, Lu Y, Tan L, Dongxu Z and Zhang K: The long non-coding RNA-ROR promotes osteosarcoma progression by targeting miR-206. J Cell Mol Med. 23:1865–1872. 2019.PubMed/NCBI View Article : Google Scholar

17 

Cheng Z, Shu H, Cui Y, Zhang Q, Zhao B, Pan D, Chao Q and Wang D: MiR-424-5p inhibits proliferation, invasion and promotes apoptosis and predicts good prognosis in glioma by directly targeting BFAR. Pathol Oncol Res. 26:2327–2335. 2020.PubMed/NCBI View Article : Google Scholar

18 

Wu JB, Yang B, Zhang Y, Feng X, He B, Xie H, Zhou L, Wu J and Zheng S: miR-424-5p represses the metastasis and invasion of intrahepatic cholangiocarcinoma by targeting ARK5. Int J Biol Sci. 15:1591–1599. 2019.PubMed/NCBI View Article : Google Scholar

19 

Wang K, Zhu G, Bao S and Chen S: Long non-coding RNA LINC00511 mediates the effects of ESR1 on proliferation and invasion of ovarian cancer through miR-424-5p and miR-370-5p. Cancer Manag Res. 11:10807–10819. 2019.PubMed/NCBI View Article : Google Scholar

20 

Long XH, Mao JH, Peng AF, Zhou Y, Huang SH and Liu ZL: Tumor suppressive microRNA-424 inhibits osteosarcoma cell migration and invasion via targeting fatty acid synthase. Exp Ther Med. 5:1048–1052. 2013.PubMed/NCBI View Article : Google Scholar

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

22 

Huang Y, Liu W, He B, Wang L, Zhang F, Shu H and Sun L: Exosomes derived from bone marrow mesenchymal stem cells promote osteosarcoma development by activating oncogenic autophagy. J Bone Oncol. 21(100280)2020.PubMed/NCBI View Article : Google Scholar

23 

Yang C, Wu K, Wang S and Wei G: Long non-coding RNA XIST promotes osteosarcoma progression by targeting YAP via miR-195-5p. J Cell Biochem. 119:5646–5656. 2018.PubMed/NCBI View Article : Google Scholar

24 

Zhao J, Zhang C, Gao Z, Wu H, Gu R and Jiang R: Long non-coding RNA ASBEL promotes osteosarcoma cell proliferation, migration, and invasion by regulating microRNA-21. J Cell Biochem. 119:6461–6469. 2018.PubMed/NCBI View Article : Google Scholar

25 

Xu R, Feng F, Yu X, Liu Z and Lao L: LncRNA SNHG4 promotes tumour growth by sponging miR-224-3p and predicts poor survival and recurrence in human osteosarcoma. Cell Prolif. 51(e12515)2018.PubMed/NCBI View Article : Google Scholar

26 

Dong P, Xiong Y, Yue J, Hanley SJB and Watari H: miR-34a, miR-424 and miR-513 inhibit MMSET expression to repress endometrial cancer cell invasion and sphere formation. Oncotarget. 9:23253–23263. 2018.PubMed/NCBI View Article : Google Scholar

27 

Wang J, Wang S, Zhou J and Qian Q: miR-424-5p regulates cell proliferation, migration and invasion by targeting doublecortin-like kinase 1 in basal-like breast cancer. Biomed Pharmacother. 102:147–152. 2018.PubMed/NCBI View Article : Google Scholar

28 

Fang Y, Liang X, Xu J and Cai X: miR-424 targets AKT3 and PSAT1 and has a tumor-suppressive role in human colorectal cancer. Cancer Manag Res. 10:6537–6547. 2018.PubMed/NCBI View Article : Google Scholar

29 

Shekhar R, Priyanka P, Kumar P, Ghosh T, Khan MM, Nagarajan P and Saxena S: The microRNAs miR-449a and miR-424 suppress osteosarcoma by targeting cyclin A2 expression. J Biol Chem. 294:4381–4400. 2019.PubMed/NCBI View Article : Google Scholar

30 

Yamanaka Y, Friess H, Buchler M, Beger HG, Uchida E, Onda M, Kobrin MS and Korc M: Overexpression of acidic and basic fibroblast growth factors in human pancreatic cancer correlates with advanced tumor stage. Cancer Res. 53:5289–5296. 1993.PubMed/NCBI

31 

Berger W, Setinek U, Mohr T, Kindas-Mügge I, Vetterlein M, Dekan G, Eckersberger F, Caldas C and Micksche M: Evidence for a role of FGF-2 and FGF receptors in the proliferation of non-small cell lung cancer cells. Int J Cancer. 83:415–423. 1999.PubMed/NCBI View Article : Google Scholar

32 

Gazzaniga P, Gandini O, Gradilone A, Silvestri I, Giuliani L, Magnanti M, Gallucci M, Saccani G, Frati L and Agliano AM: Detection of basic fibroblast growth factor mRNA in urinary bladder cancer: Correlation with local relapses. Int J Oncol. 14:1123–1127. 1999.PubMed/NCBI View Article : Google Scholar

33 

Zhang QH, Xu P, Lu YX and Dou HT: Acidic and basic fibroblast growth factor expression levels in cervical cancer and their effects on tumor cell proliferation. Genet Mol Res. 15:2016.PubMed/NCBI View Article : Google Scholar

34 

Cheng Z, Ma R, Tan W and Zhang L: MiR-152 suppresses the proliferation and invasion of NSCLC cells by inhibiting FGF2. Exp Mol Med. 46(e112)2014.PubMed/NCBI View Article : Google Scholar

35 

Sun XH, Geng XL, Zhang J and Zhang C: miRNA-646 suppresses osteosarcoma cell metastasis by downregulating fibroblast growth factor 2 (FGF2). Tumour Biol. 36:2127–2134. 2015.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Li Z, Wang X and Liang S: Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 <em>in vitro</em>. Exp Ther Med 21: 325, 2021.
APA
Li, Z., Wang, X., & Liang, S. (2021). Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 <em>in vitro</em>. Experimental and Therapeutic Medicine, 21, 325. https://doi.org/10.3892/etm.2021.9756
MLA
Li, Z., Wang, X., Liang, S."Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 <em>in vitro</em>". Experimental and Therapeutic Medicine 21.4 (2021): 325.
Chicago
Li, Z., Wang, X., Liang, S."Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 <em>in vitro</em>". Experimental and Therapeutic Medicine 21, no. 4 (2021): 325. https://doi.org/10.3892/etm.2021.9756
Copy and paste a formatted citation
x
Spandidos Publications style
Li Z, Wang X and Liang S: Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 <em>in vitro</em>. Exp Ther Med 21: 325, 2021.
APA
Li, Z., Wang, X., & Liang, S. (2021). Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 <em>in vitro</em>. Experimental and Therapeutic Medicine, 21, 325. https://doi.org/10.3892/etm.2021.9756
MLA
Li, Z., Wang, X., Liang, S."Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 <em>in vitro</em>". Experimental and Therapeutic Medicine 21.4 (2021): 325.
Chicago
Li, Z., Wang, X., Liang, S."Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 <em>in vitro</em>". Experimental and Therapeutic Medicine 21, no. 4 (2021): 325. https://doi.org/10.3892/etm.2021.9756
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