Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 <em>in vitro</em>

Li,Zhuokai; Wang,Xiaohe; Liang,Shuofu

doi:10.3892/etm.2021.9756

Long non‑coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA‑424‑5p/FGF2 in vitro

Authors:
- Zhuokai Li
- Xiaohe Wang
- Shuofu Liang
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Affiliations: Department of Orthopaedics, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, P.R. China, Department of Orthopaedics, Jinan Zhangqiu District Hospital of TCM, Jinan, Shandong 250200, P.R. China, Department of Orthopaedics, Zibo Zhoucun People's Hospital, Zibo, Shandong 255300, P.R. China
Published online on: February 5, 2021 https://doi.org/10.3892/etm.2021.9756
Article Number: 325

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Abstract

The aim of the present study was to clarify the effect of long non‑coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) on the proliferation, migration and invasion of osteosarcoma (OS) cells and to explore the potential underlying mechanisms. The expression levels of SNHG1, microRNA (miR)‑424‑5p and fibroblast growth factor 2 (FGF2) in OS tissues and cells were detected using reverse transcription‑quantitative polymerase chain reaction. OS cell proliferation, migration and invasion were analysed by MTT, wound healing and Transwell invasion assays, respectively. The targeting relationships between SNHG1 and miR‑424‑5p, as well as between miR‑424‑5p and FGF2, were confirmed using RNA‑binding protein immunoprecipitation and/or dual‑luciferase reporter gene assays. The results demonstrated that the expression levels of SNHG1 and FGF2 were upregulated, whereas the expression of miR‑424‑5p was downregulated in OS tissues and cells. The silencing of SNHG1 significantly inhibited the proliferation, migration and invasion of OS cells. Additionally, FGF2 was shown to be a target of miR‑424‑5p, which in turn, was a target of SNHG1. miR‑424‑5p silencing and FGF2 overexpression both reversed the suppressive effects of SNHG1 knockdown on the proliferation, migration and invasion of OS cells. Thus, the silencing of SNHG1 may inhibit the proliferation, migration and invasion of OS cells by regulating the miR‑424‑5p/FGF2 axis.

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