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MicroRNA‑22 contributes to dexamethasone‑induced osteoblast differentiation inhibition and dysfunction through targeting caveolin‑3 expression in osteoblastic cells

  • Authors:
    • Peng Li
    • Weiwei Mao
    • Shuai Zhang
    • Liang Zhang
    • Zhirong Chen
    • Zhidong Lu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Clinical Skill Center of Yinchuan First People's Hospital, Yinchuan, Ningxia 750001, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 336
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    Published online on: February 8, 2021
       https://doi.org/10.3892/etm.2021.9767
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Abstract

Osteoporosis is a common complication of long‑term use of glucocorticoids (GCs) characterized by the loss of bone mass and damage of the microarchitecture as well as osteoblast dysfunction. Previous studies have demonstrated that microRNA‑22 (miR‑22) is the negative modulator of osteogenesis that may target caveolin‑3 (CAV3), which has been reported to enhance bone formation and inhibit the progression of osteoporosis as well as apoptosis. The present study aimed to investigate whether miR‑22 may be involved in dexamethasone (DEX)‑induced inhibition of osteoblast differentiation and dysfunction by regulating CAV3 expression. Reverse transcription‑quantitative PCR (RT‑qPCR) was performed to measure the expression of miR‑22 and western blotting was performed to determine protein levels. The results demonstrated that miR‑22 expression was upregulated in DEX‑treated osteoblastic cells compared with the control group. In addition, miR‑22 mimic aggravated, whereas miR‑22 inhibitor mitigated DEX‑induced damage in osteoblastic cells compared with the control groups. Additionally, CAV3 was identified as the target of miR‑22 in osteoblasts using RT‑qPCR, western blotting and dual‑luciferase reporter gene assay analysis. The results also demonstrated that silencing of CAV3 blocked the beneficial effects of miR‑22 inhibitor against DEX‑induced cell damage and apoptosis in osteoblasts, as evidenced by the increased expression levels of cleaved caspase‑3, Bax and alkaline phosphatase activity as well as decreased cell viability and Bcl‑2 levels. Collectively, these results indicate a novel molecular mechanism by which miR‑22 contributes to DEX‑induced osteoblast dysfunction and apoptosis via the miR‑22/CAV3 pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Li P, Mao W, Zhang S, Zhang L, Chen Z and Lu Z: MicroRNA‑22 contributes to dexamethasone‑induced osteoblast differentiation inhibition and dysfunction through targeting caveolin‑3 expression in osteoblastic cells. Exp Ther Med 21: 336, 2021.
APA
Li, P., Mao, W., Zhang, S., Zhang, L., Chen, Z., & Lu, Z. (2021). MicroRNA‑22 contributes to dexamethasone‑induced osteoblast differentiation inhibition and dysfunction through targeting caveolin‑3 expression in osteoblastic cells. Experimental and Therapeutic Medicine, 21, 336. https://doi.org/10.3892/etm.2021.9767
MLA
Li, P., Mao, W., Zhang, S., Zhang, L., Chen, Z., Lu, Z."MicroRNA‑22 contributes to dexamethasone‑induced osteoblast differentiation inhibition and dysfunction through targeting caveolin‑3 expression in osteoblastic cells". Experimental and Therapeutic Medicine 21.4 (2021): 336.
Chicago
Li, P., Mao, W., Zhang, S., Zhang, L., Chen, Z., Lu, Z."MicroRNA‑22 contributes to dexamethasone‑induced osteoblast differentiation inhibition and dysfunction through targeting caveolin‑3 expression in osteoblastic cells". Experimental and Therapeutic Medicine 21, no. 4 (2021): 336. https://doi.org/10.3892/etm.2021.9767
Copy and paste a formatted citation
x
Spandidos Publications style
Li P, Mao W, Zhang S, Zhang L, Chen Z and Lu Z: MicroRNA‑22 contributes to dexamethasone‑induced osteoblast differentiation inhibition and dysfunction through targeting caveolin‑3 expression in osteoblastic cells. Exp Ther Med 21: 336, 2021.
APA
Li, P., Mao, W., Zhang, S., Zhang, L., Chen, Z., & Lu, Z. (2021). MicroRNA‑22 contributes to dexamethasone‑induced osteoblast differentiation inhibition and dysfunction through targeting caveolin‑3 expression in osteoblastic cells. Experimental and Therapeutic Medicine, 21, 336. https://doi.org/10.3892/etm.2021.9767
MLA
Li, P., Mao, W., Zhang, S., Zhang, L., Chen, Z., Lu, Z."MicroRNA‑22 contributes to dexamethasone‑induced osteoblast differentiation inhibition and dysfunction through targeting caveolin‑3 expression in osteoblastic cells". Experimental and Therapeutic Medicine 21.4 (2021): 336.
Chicago
Li, P., Mao, W., Zhang, S., Zhang, L., Chen, Z., Lu, Z."MicroRNA‑22 contributes to dexamethasone‑induced osteoblast differentiation inhibition and dysfunction through targeting caveolin‑3 expression in osteoblastic cells". Experimental and Therapeutic Medicine 21, no. 4 (2021): 336. https://doi.org/10.3892/etm.2021.9767
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