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Ckip‑1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5

  • Authors:
    • Xin Huang
    • Jianfei Liang
    • Ye Gao
    • Yan Hou
    • Yu Song
    • Liang Kong
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Orthodontics, Qingdao Stomatological Hospital, Qingdao, Shandong 266001, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 342
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    Published online on: February 10, 2021
       https://doi.org/10.3892/etm.2021.9773
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Abstract

Casein kinase‑2 interaction protein‑1 (Ckip‑1) is a negative regulator of bone formation. The identification of novel Ckip‑1‑related targets and their associated signaling pathways that regulate mesenchymal stem cell (MSC) osteogenic differentiation is required. The present study aimed to evaluate the effects of Ckip‑1 knockdown on C3H10T1/2 MSC proliferation and osteogenic differentiation, and to explore the role of the canonical Wnt‑signaling receptor Lrp5. Ckip‑1‑knockdown (shCkip‑1), Ckip‑1‑overexpression (Ckip‑1) and their corresponding control [shCtrl and empty vector (EV), respectively] cell groups were used in the present study. Immunofluorescence localization of Ckip‑1 was observed. The expression of the key molecules of the canonical Wnt signaling pathway was examined in C3H10T1/2 cells following osteogenic induction. Moreover, the effects of Lrp5 knockdown in the presence or absence of Ckip‑1 knockdown were examined on C3H10T1/2 cell proliferation and osteogenic differentiation. The results indicated an increase in cell proliferation and osteogenic differentiation in the shCkip‑1 group compared with the shCtrl group. The expression levels of LDL receptor related protein 5 (Lrp5), lymphoid enhancer binding factor 1 (Lef1) and transcription factor 1 in C3H10T1/2 cells were significantly increased in shCkip‑1 cells following 7‑day osteoinduction compared with shCtrl cells. Moreover, the involvement of Lrp5 in shCkip‑1‑induced osteogenic differentiation of C3H10T1/2 cells was further verified. The results indicated that Ckip‑1 reduced C3H10T1/2 MSC proliferation and osteogenic differentiation via the canonical Wnt‑signaling receptor Lrp5, which is essential for the improvement of bone tissue engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Huang X, Liang J, Gao Y, Hou Y, Song Y and Kong L: Ckip‑1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5. Exp Ther Med 21: 342, 2021.
APA
Huang, X., Liang, J., Gao, Y., Hou, Y., Song, Y., & Kong, L. (2021). Ckip‑1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5. Experimental and Therapeutic Medicine, 21, 342. https://doi.org/10.3892/etm.2021.9773
MLA
Huang, X., Liang, J., Gao, Y., Hou, Y., Song, Y., Kong, L."Ckip‑1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5". Experimental and Therapeutic Medicine 21.4 (2021): 342.
Chicago
Huang, X., Liang, J., Gao, Y., Hou, Y., Song, Y., Kong, L."Ckip‑1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5". Experimental and Therapeutic Medicine 21, no. 4 (2021): 342. https://doi.org/10.3892/etm.2021.9773
Copy and paste a formatted citation
x
Spandidos Publications style
Huang X, Liang J, Gao Y, Hou Y, Song Y and Kong L: Ckip‑1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5. Exp Ther Med 21: 342, 2021.
APA
Huang, X., Liang, J., Gao, Y., Hou, Y., Song, Y., & Kong, L. (2021). Ckip‑1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5. Experimental and Therapeutic Medicine, 21, 342. https://doi.org/10.3892/etm.2021.9773
MLA
Huang, X., Liang, J., Gao, Y., Hou, Y., Song, Y., Kong, L."Ckip‑1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5". Experimental and Therapeutic Medicine 21.4 (2021): 342.
Chicago
Huang, X., Liang, J., Gao, Y., Hou, Y., Song, Y., Kong, L."Ckip‑1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5". Experimental and Therapeutic Medicine 21, no. 4 (2021): 342. https://doi.org/10.3892/etm.2021.9773
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