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CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis

  • Authors:
    • Xianhui Wen
    • Junhua Zhang
    • Wenjuan Yang
    • Xinmin Nie
    • Rong Gui
    • Dongyong Shan
    • Rong Huang
    • Hongyu Deng
  • View Affiliations / Copyright

    Affiliations: Department of Blood Transfusion, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Key Laboratory of Translational Radiation Oncology, Department of Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha, Hunan 410013, P.R. China, Department of Laboratory Medicine, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Department of Oncology, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Department of Laboratory Medicine, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China
    Copyright: © Wen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 355
    |
    Published online on: February 11, 2021
       https://doi.org/10.3892/etm.2021.9786
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Abstract

Currently, bone marrow transplantation remains the basic treatment for various hematological tumors and irradiation is one of the most important pretreatment methods. However, irradiation pretreatment may result in damage to bone mesenchymal stem cells (BMSCs). The present study aimed to investigate the effect of circular RNA-016901 (circ‑016901) on the injury of irradiation-induced BMSCs and the underlying mechanism. The expression levels of circ‑016901, microRNA-1249-5p (miR-1249-5p) and homeodomain interacting protein kinase 2 (HIPK2) in irradiation-induced mouse BMSCs at various irradiation doses were detected via reverse transcription-quantitative PCR (RT-qPCR). The effect of circ-016901 on cell proliferation was examined using Cell Counting Kit-8 assays following silencing or overexpression of circ-016901. Cell apoptosis was detected by flow cytometry and caspase-3/7 activity. The expression of autophagy-related markers, including Beclin-1 and LC3-II/I, was detected at the mRNA and protein levels by RT-qPCR and western blotting, respectively. Irradiation treatment upregulated the expression of circ-016901 and HIPK2 and downregulated miR-1249-5p expression. The expression levels of LC3-II/I and Beclin-1 in BMSCs were downregulated in a dose-dependent manner. Silencing of circ-016901 promoted proliferation of irradiation-induced BMSCs and attenuated irradiation-induced apoptosis. Moreover, silencing of circ-016901 elevated the expressions of LC3-II/I and Beclin-1 in irradiation-induced BMSCs. Similar results were obtained with miR-1249-5p overexpression and HIPK2 silencing. These results demonstrated that circ-016901 silencing attenuated injury in irradiation-induced mouse BMSCs by regulating the miR‑1249-5p/HIPK2 axis, providing a novel target for future research on the mechanism of radiation resistance in BMSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Wen X, Zhang J, Yang W, Nie X, Gui R, Shan D, Huang R and Deng H: CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis. Exp Ther Med 21: 355, 2021.
APA
Wen, X., Zhang, J., Yang, W., Nie, X., Gui, R., Shan, D. ... Deng, H. (2021). CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis. Experimental and Therapeutic Medicine, 21, 355. https://doi.org/10.3892/etm.2021.9786
MLA
Wen, X., Zhang, J., Yang, W., Nie, X., Gui, R., Shan, D., Huang, R., Deng, H."CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis". Experimental and Therapeutic Medicine 21.4 (2021): 355.
Chicago
Wen, X., Zhang, J., Yang, W., Nie, X., Gui, R., Shan, D., Huang, R., Deng, H."CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis". Experimental and Therapeutic Medicine 21, no. 4 (2021): 355. https://doi.org/10.3892/etm.2021.9786
Copy and paste a formatted citation
x
Spandidos Publications style
Wen X, Zhang J, Yang W, Nie X, Gui R, Shan D, Huang R and Deng H: CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis. Exp Ther Med 21: 355, 2021.
APA
Wen, X., Zhang, J., Yang, W., Nie, X., Gui, R., Shan, D. ... Deng, H. (2021). CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis. Experimental and Therapeutic Medicine, 21, 355. https://doi.org/10.3892/etm.2021.9786
MLA
Wen, X., Zhang, J., Yang, W., Nie, X., Gui, R., Shan, D., Huang, R., Deng, H."CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis". Experimental and Therapeutic Medicine 21.4 (2021): 355.
Chicago
Wen, X., Zhang, J., Yang, W., Nie, X., Gui, R., Shan, D., Huang, R., Deng, H."CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis". Experimental and Therapeutic Medicine 21, no. 4 (2021): 355. https://doi.org/10.3892/etm.2021.9786
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