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Caesalpinia sappan induces apoptotic cell death in ectopic endometrial 12Z cells through suppressing pyruvate dehydrogenase kinase 1 expression

  • Authors:
    • Bo-Sung Kim
    • Tae-Wook Chung
    • Hee-Jung Choi
    • Sung-Jin Bae
    • Hye-Rin Cho
    • Syng-Ook Lee
    • Jung-Hye Choi
    • Jong Kil Joo
    • Ki-Tae Ha
  • View Affiliations / Copyright

    Affiliations: Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea, Healthy Aging Korean Medical Research Center, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea, Department of Food Science and Technology, Keimyung University, Daegu 42601, Republic of Korea, Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02453, Republic of Korea, Department of Obstetrics and Gynecology, School of Medicine, Pusan National University Hospital, Busan 49241, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 357
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    Published online on: February 11, 2021
       https://doi.org/10.3892/etm.2021.9788
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Abstract

Endometriosis is a common gynecological disease defined as the growth of endometrial tissues outside the uterus. Although the mechanism underlying the progression of endometriosis has not been fully elucidated, cancer‑like aerobic glycolysis is considered to mediate the elevated growth and resistance to apoptosis of endometriotic cells. The heartwood of Caesalpinia sappan L. (family Leguminosae) is a herbal medicinal product used to treat gynecological symptoms, including algomenorrhea and amenorrhea. The results of the present study revealed that endometriotic 12Z cells exhibited more rapid growth than normal endometrial cells (THES). The expression levels of pyruvate dehydrogenase kinase (PDK)1 and 3 and lactate production were higher in 12Z cells than in THES cells. In addition, the 12Z cells were more sensitive to the cytotoxicity of the aqueous extract of C. sappan heartwood (CS) than the THES cells. CS inhibited lactate production and phosphorylation of pyruvate dehydrogenase A by reducing the expression of PDK1. CS also increased mitochondrial reactive oxygen species (ROS) levels, decreased mitochondrial membrane potential and consequently stimulated the apoptosis of 12Z cells. CS‑induced cell death was substantially inhibited by exogenous PDK1 expression. In conclusion, CS may be a novel drug candidate for treating endometriosis by inhibiting aerobic glycolysis and inducing ROS‑mitochondria‑mediated apoptotic cell death.
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Copy and paste a formatted citation
Spandidos Publications style
Kim B, Chung T, Choi H, Bae S, Cho H, Lee S, Choi J, Joo JK and Ha K: <em>Caesalpinia sappan</em> induces apoptotic cell death in ectopic endometrial 12Z cells through suppressing pyruvate dehydrogenase kinase 1 expression. Exp Ther Med 21: 357, 2021.
APA
Kim, B., Chung, T., Choi, H., Bae, S., Cho, H., Lee, S. ... Ha, K. (2021). <em>Caesalpinia sappan</em> induces apoptotic cell death in ectopic endometrial 12Z cells through suppressing pyruvate dehydrogenase kinase 1 expression. Experimental and Therapeutic Medicine, 21, 357. https://doi.org/10.3892/etm.2021.9788
MLA
Kim, B., Chung, T., Choi, H., Bae, S., Cho, H., Lee, S., Choi, J., Joo, J. K., Ha, K."<em>Caesalpinia sappan</em> induces apoptotic cell death in ectopic endometrial 12Z cells through suppressing pyruvate dehydrogenase kinase 1 expression". Experimental and Therapeutic Medicine 21.4 (2021): 357.
Chicago
Kim, B., Chung, T., Choi, H., Bae, S., Cho, H., Lee, S., Choi, J., Joo, J. K., Ha, K."<em>Caesalpinia sappan</em> induces apoptotic cell death in ectopic endometrial 12Z cells through suppressing pyruvate dehydrogenase kinase 1 expression". Experimental and Therapeutic Medicine 21, no. 4 (2021): 357. https://doi.org/10.3892/etm.2021.9788
Copy and paste a formatted citation
x
Spandidos Publications style
Kim B, Chung T, Choi H, Bae S, Cho H, Lee S, Choi J, Joo JK and Ha K: <em>Caesalpinia sappan</em> induces apoptotic cell death in ectopic endometrial 12Z cells through suppressing pyruvate dehydrogenase kinase 1 expression. Exp Ther Med 21: 357, 2021.
APA
Kim, B., Chung, T., Choi, H., Bae, S., Cho, H., Lee, S. ... Ha, K. (2021). <em>Caesalpinia sappan</em> induces apoptotic cell death in ectopic endometrial 12Z cells through suppressing pyruvate dehydrogenase kinase 1 expression. Experimental and Therapeutic Medicine, 21, 357. https://doi.org/10.3892/etm.2021.9788
MLA
Kim, B., Chung, T., Choi, H., Bae, S., Cho, H., Lee, S., Choi, J., Joo, J. K., Ha, K."<em>Caesalpinia sappan</em> induces apoptotic cell death in ectopic endometrial 12Z cells through suppressing pyruvate dehydrogenase kinase 1 expression". Experimental and Therapeutic Medicine 21.4 (2021): 357.
Chicago
Kim, B., Chung, T., Choi, H., Bae, S., Cho, H., Lee, S., Choi, J., Joo, J. K., Ha, K."<em>Caesalpinia sappan</em> induces apoptotic cell death in ectopic endometrial 12Z cells through suppressing pyruvate dehydrogenase kinase 1 expression". Experimental and Therapeutic Medicine 21, no. 4 (2021): 357. https://doi.org/10.3892/etm.2021.9788
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