Open Access

MSK1 downregulation is involved in inflammatory responses following subarachnoid hemorrhage in rats

  • Authors:
    • Bo Ning
    • Zhen Li
    • Lei Ning
    • Jun Wu
    • Xin Chen
    • Pengjun Jiang
    • Fuxin Lin
    • Bing Zhao
  • View Affiliations

  • Published online on: February 18, 2021     https://doi.org/10.3892/etm.2021.9795
  • Article Number: 364
  • Copyright: © Ning et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Subarachnoid hemorrhage (SAH) is a life‑threatening neurological disease. Recently, inflammatory factors have been confirmed to be responsible for the brain damage associated with SAH. Therefore, studying the post‑SAH inflammatory reaction may clarify the mechanism of SAH. Mitogen and stress‑activated protein kinase 1 (MSK1) causes the phosphorylation of NF‑κB and regulates the activity of pro‑inflammatory transcription factors. The present study aimed to identify the potential role of MSK1 in inflammation and brain damage development following SAH. A cisterna magna blood injection model was established in Sprague‑Dawley rats. Hematoxylin and eosin staining, reverse transcription‑quantitative polymerase chain reaction assays and double immunofluorescence staining were used to analyze the role of MSK1, IL‑1β and TNF‑α in the inflammatory process after SAH. In a model of lipopolysaccharide‑induced astrocyte inflammation, the effect of overexpressing MSK1 overexpression was analyzed by western blot analysis. The results demonstrated that MSK1 expression were negatively correlated with TNF‑α and IL‑1β expression levels, and reached peak levels 2 days after TNF‑α and IL‑1β. The double immunofluorescence staining results showed that the expression of MSK1 was in the same plane of view as TNF‑α and IL‑1β in the brain cortex. Furthermore, the in vitro studies indicated that the overexpression of MSK1 inhibited the expression of TNF‑α and IL‑1β following LPS challenge. These results imply that MSK1 may be involved in the inflammatory reaction following SAH, and may potentially serve as a negative regulator of inflammation.
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April-2021
Volume 21 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ning B, Li Z, Ning L, Wu J, Chen X, Jiang P, Lin F and Zhao B: MSK1 downregulation is involved in inflammatory responses following subarachnoid hemorrhage in rats. Exp Ther Med 21: 364, 2021.
APA
Ning, B., Li, Z., Ning, L., Wu, J., Chen, X., Jiang, P. ... Zhao, B. (2021). MSK1 downregulation is involved in inflammatory responses following subarachnoid hemorrhage in rats. Experimental and Therapeutic Medicine, 21, 364. https://doi.org/10.3892/etm.2021.9795
MLA
Ning, B., Li, Z., Ning, L., Wu, J., Chen, X., Jiang, P., Lin, F., Zhao, B."MSK1 downregulation is involved in inflammatory responses following subarachnoid hemorrhage in rats". Experimental and Therapeutic Medicine 21.4 (2021): 364.
Chicago
Ning, B., Li, Z., Ning, L., Wu, J., Chen, X., Jiang, P., Lin, F., Zhao, B."MSK1 downregulation is involved in inflammatory responses following subarachnoid hemorrhage in rats". Experimental and Therapeutic Medicine 21, no. 4 (2021): 364. https://doi.org/10.3892/etm.2021.9795