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MicroRNA‑185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator‑activated receptor β

  • Authors:
    • Jingzhe Yang
    • Pingyang Deng
    • Yonggang Qi
    • Xinshu Feng
    • Hailing Wen
    • Fengping Chen
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    Affiliations: Department of Burn and Plastic Surgery, Affiliated Hospital of Chengde Medical University, South Wing Hospital of Chengde Medical College, Chengde, Hebei 067000, P.R. China, Department of Burn and Plastic Surgery, Bayingol Mongolia Autonomous Prefecture People's Hospital, Urumqi, Xinjiang Uyghur Autonomous Region 841000, P.R. China, Department of General Surgery, Bayingol Mongolia Autonomous Prefecture People's Hospital, Urumqi, Xinjiang Uyghur Autonomous Region 841000, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 366
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    Published online on: February 18, 2021
       https://doi.org/10.3892/etm.2021.9797
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Abstract

Proliferation and migration of keratinocytes are major processes of skin wound repair after injury. It has been indicated that microRNAs (miRNAs/miRs) are associated with the proliferation and migration of keratinocytes. However, the mechanism by which miR‑185 affects these processes in keratinocytes remains unclear. In the present study, the expression level of miR‑185 and peroxisome proliferator‑activated receptor β (PPARβ) was examined by reverse transcription‑quantitative PCR in HaCaT keratinocytes. Cell proliferation was evaluated using Cell Counting Kit‑8 and colony formation assays. Western blot analysis was used to detect the levels of cell proliferation, migration and PI3K/AKT signaling pathway‑associated proteins. In addition, the migratory capacity of the cells was determined using Transwell assay. The target gene of miR‑185 was verified using dual‑luciferase reporter assay. The results indicated that overexpression of miR‑185 inhibited proliferation, migration and activation of the PI3K/AKT signaling pathway in HaCaT keratinocytes. PPARβ was indicated to be a target of miR‑185 and its overexpression promoted the proliferation and migration of HaCaT keratinocytes, while its knockdown exhibited the adverse effects. Furthermore, PI3K inhibitor LY294002 inhibited activation of the PI3K/AKT signaling pathway and decreased the proliferation and migration of HaCaT keratinocytes. In addition, overexpressed PPARβ reversed the suppressive effects of miR‑185 overexpression on proliferation, migration and activation of the PI3K/AKT signaling pathway. In conclusion, the results of the present study demonstrated that miR‑185 suppressed activation of the PI3K/AKT signaling pathway via targeting PPARβ, thereby regulating proliferation and migration in HaCaT keratinocytes. The present study provided a novel theoretical basis for the use of miR‑185 as a target in wound repair.
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1 

Krafts KP: Tissue repair: The hidden drama. Organogenesis. 6:225–233. 2010.PubMed/NCBI View Article : Google Scholar

2 

Reinke JM and Sorg H: Wound repair and regeneration. Eur Surg Res. 49:35–43. 2012.PubMed/NCBI View Article : Google Scholar

3 

Gurtner GC, Werner S, Barrandon Y and Longaker MT: Wound repair and regeneration. Nature. 453:314–321. 2008.PubMed/NCBI View Article : Google Scholar

4 

Kiwanuka E, Hackl F, Caterson EJ, Nowinski D, Junker JP, Gerdin B and Eriksson E: CCN2 is transiently expressed by keratinocytes during re-epithelialization and regulates keratinocyte migration in vitro by the ras-MEK-ERK signaling pathway. J Surg Res. 185:e109–e119. 2013.PubMed/NCBI View Article : Google Scholar

5 

Shilo S, Roy S, Khanna S and Sen CK: MicroRNA in cutaneous wound healing: A new paradigm. DNA Cell Biol. 26:227–237. 2007.PubMed/NCBI View Article : Google Scholar

6 

Lai WF and Siu PM: MicroRNAs as regulators of cutaneous wound healing. J Biosci. 39:519–524. 2014.PubMed/NCBI View Article : Google Scholar

7 

Fahs F, Bi X, Yu FS, Zhou L and Mi QS: New insights into microRNAs in skin wound healing. IUBMB Life. 67:889–896. 2015.PubMed/NCBI View Article : Google Scholar

8 

Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009.PubMed/NCBI View Article : Google Scholar

9 

Jiang X, Tsitsiou E, Herrick SE and Lindsay MA: MicroRNAs and the regulation of fibrosis. FEBS J. 277:2015–2021. 2010.PubMed/NCBI View Article : Google Scholar

10 

Yang W and Yee AJ: Versican 3'-untranslated region (3'UTR) promotes dermal wound repair and fibroblast migration by regulating miRNA activity. Biochim Biophys Acta. 1843:1373–1385. 2014.PubMed/NCBI View Article : Google Scholar

11 

Sorensen HN, Treuter E and Gustafsson JA: Regulation of peroxisome proliferator-activated receptors. Vitam Horm. 54:121–166. 1998.PubMed/NCBI View Article : Google Scholar

12 

Wang D, Fu L, Ning W, Guo L, Sun X, Dey SK, Chaturvedi R, Wilson KT and DuBois RN: Peroxisome proliferator-activated receptor delta promotes colonic inflammation and tumor growth. Proc Natl Acad Sci USA. 111:7084–7089. 2014.PubMed/NCBI View Article : Google Scholar

13 

Hollingshead HE, Borland MG, Billin AN, Willson TM, Gonzalez FJ and Peters JM: Ligand activation of peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) and inhibition of cyclooxygenase 2 (COX2) attenuate colon carcinogenesis through independent signaling mechanisms. Carcinogenesis. 29:169–176. 2008.PubMed/NCBI View Article : Google Scholar

14 

Berger J, Leibowitz MD, Doebber TW, Elbrecht A, Zhang B, Zhou G, Biswas C, Cullinan CA, Hayes NS, Li Y, et al: Novel peroxisome proliferator-activated receptor (PPAR) gamma and PPARdelta ligands produce distinct biological effects. J Biol Chem. 274:6718–6725. 1999.PubMed/NCBI View Article : Google Scholar

15 

Di-Poi N, Michalik L, Tan NS, Desvergne B and Wahli W: The anti-apoptotic role of PPARbeta contributes to efficient skin wound healing. J Steroid Biochem Mol Biol. 85:257–265. 2003.PubMed/NCBI View Article : Google Scholar

16 

Tan NS, Michalik L, Desvergne B and Wahli W: Peroxisome proliferator-activated receptor-beta as a target for wound healing drugs. Expert Opin Ther Targets. 8:39–48. 2004.PubMed/NCBI View Article : Google Scholar

17 

Tan NS, Michalik L, Di-Poi N, Desvergne B and Wahli W: Critical roles of the nuclear receptor PPARbeta (peroxisome-proliferator-activated receptor beta) in skin wound healing. Biochem Soc Trans. 32:97–102. 2004.PubMed/NCBI View Article : Google Scholar

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

19 

Wang L, Zhang ZG, Zhang RL, Gregg SR, Hozeska-Solgot A, LeTourneau Y, Wang Y and Chopp M: Matrix metalloproteinase 2 (MMP2) and MMP9 secreted by erythropoietin-activated endothelial cells promote neural progenitor cell migration. J Neurosci. 26:5996–6003. 2006.PubMed/NCBI View Article : Google Scholar

20 

Lai EC, Wiel C and Rubin GM: Complementary miRNA pairs suggest a regulatory role for miRNA: miRNA duplexes. RNA. 10:171–175. 2004.PubMed/NCBI View Article : Google Scholar

21 

Breitkreutz D, Mirancea N and Nischt R: Basement membranes in skin: Unique matrix structures with diverse functions? Histochem Cell Biol. 132:1–10. 2009.PubMed/NCBI View Article : Google Scholar

22 

Kondo T and Ishida Y: Molecular pathology of wound healing. Forensic Sci Int. 203:93–98. 2010.PubMed/NCBI View Article : Google Scholar

23 

Boukamp P, Petrussevska RT, Breitkreutz D, Hornung J, Markham A and Fusenig NE: Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol. 106:761–771. 1988.PubMed/NCBI View Article : Google Scholar

24 

Colombo I, Sangiovanni E, Maggio R, Mattozzi C, Zava S, Corbett Y, Fumagalli M, Carlino C, Corsetto PA, Scaccabarozzi D, et al: HaCaT cells as a reliable in vitro differentiation model to dissect the inflammatory/repair response of human keratinocytes. Mediators Inflamm. 2017(7435621)2017.PubMed/NCBI View Article : Google Scholar

25 

Liu M, Lang N, Chen X, Tang Q, Liu S, Huang J, Zheng Y and Bi F: miR-185 targets RhoA and Cdc42 expression and inhibits the proliferation potential of human colorectal cells. Cancer Lett. 301:151–160. 2011.PubMed/NCBI View Article : Google Scholar

26 

Qu F, Cui X, Hong Y, Wang J, Li Y, Chen L, Liu Y, Gao Y, Xu D and Wang Q: MicroRNA-185 suppresses proliferation, invasion, migration, and tumorigenicity of human prostate cancer cells through targeting androgen receptor. Mol Cell Biochem. 377:121–130. 2013.PubMed/NCBI View Article : Google Scholar

27 

Zuo C, Liang P and Huang X: Role of PPARbeta in fibroblast response to heat injury. Indian J Biochem Biophys. 49:219–227. 2012.PubMed/NCBI

28 

Michalik L, Desvergne B, Tan NS, Basu-Modak S, Escher P, Rieusset J, Peters JM, Kaya G, Gonzalez FJ, Zakany J, et al: Impaired skin wound healing in peroxisome proliferator-activated receptor (PPAR)alpha and PPARbeta mutant mice. J Cell Biol. 154:799–814. 2001.PubMed/NCBI View Article : Google Scholar

29 

Tan NS, Michalik L, Noy N, Yasmin R, Pacot C, Heim M, Fluhmann B, Desvergne B and Wahli W: Critical roles of PPAR beta/delta in keratinocyte response to inflammation. Genes Dev. 15:3263–3277. 2001.PubMed/NCBI View Article : Google Scholar

30 

Yu JS and Cui W: Proliferation, survival and metabolism: The role of PI3K/AKT/mTOR signalling in pluripotency and cell fate determination. Development. 143:3050–3060. 2016.PubMed/NCBI View Article : Google Scholar

31 

Lunardi A, Webster KA, Papa A, Padmani B, Clohessy JG, Bronson RT and Pandolfi PP: Role of aberrant PI3K pathway activation in gallbladder tumorigenesis. Oncotarget. 5:894–900. 2014.PubMed/NCBI View Article : Google Scholar

32 

Yang WL, Wu CY, Wu J and Lin HK: Regulation of Akt signaling activation by ubiquitination. Cell Cycle. 9:487–497. 2010.PubMed/NCBI View Article : Google Scholar

33 

Ye B, Jiang LL, Xu HT, Zhou DW and Li ZS: Expression of PI3K/AKT pathway in gastric cancer and its blockade suppresses tumor growth and metastasis. Int J Immunopathol Pharmacol. 25:627–636. 2012.PubMed/NCBI View Article : Google Scholar

34 

Xu W, Yang Z and Lu N: A new role for the PI3K/AKT signaling pathway in the epithelial-mesenchymal transition. Cell Adh Migr. 9:317–324. 2015.PubMed/NCBI View Article : Google Scholar

35 

Volksdorf T, Heilmann J, Eming SA, Schawjinski K, Zorn-Kruppa M, Ueck C, Vidal YSS, Windhorst S, Jucker M, Moll I and Brandner JM: Tight junction proteins claudin-1 and occludin are important for cutaneous wound healing. Am J Pathol. 187:1301–1312. 2017.PubMed/NCBI View Article : Google Scholar

36 

Galatou E, Kelly T and Lazou A: The PPARβ/δ agonist GW0742 modulates signaling pathways associated with cardiac myocyte growth via a non-genomic redox mechanism. Mol Cell Biochem. 395:145–154. 2014.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yang J, Deng P, Qi Y, Feng X, Wen H and Chen F: MicroRNA‑185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator‑activated receptor β. Exp Ther Med 21: 366, 2021.
APA
Yang, J., Deng, P., Qi, Y., Feng, X., Wen, H., & Chen, F. (2021). MicroRNA‑185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator‑activated receptor β. Experimental and Therapeutic Medicine, 21, 366. https://doi.org/10.3892/etm.2021.9797
MLA
Yang, J., Deng, P., Qi, Y., Feng, X., Wen, H., Chen, F."MicroRNA‑185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator‑activated receptor β". Experimental and Therapeutic Medicine 21.4 (2021): 366.
Chicago
Yang, J., Deng, P., Qi, Y., Feng, X., Wen, H., Chen, F."MicroRNA‑185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator‑activated receptor β". Experimental and Therapeutic Medicine 21, no. 4 (2021): 366. https://doi.org/10.3892/etm.2021.9797
Copy and paste a formatted citation
x
Spandidos Publications style
Yang J, Deng P, Qi Y, Feng X, Wen H and Chen F: MicroRNA‑185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator‑activated receptor β. Exp Ther Med 21: 366, 2021.
APA
Yang, J., Deng, P., Qi, Y., Feng, X., Wen, H., & Chen, F. (2021). MicroRNA‑185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator‑activated receptor β. Experimental and Therapeutic Medicine, 21, 366. https://doi.org/10.3892/etm.2021.9797
MLA
Yang, J., Deng, P., Qi, Y., Feng, X., Wen, H., Chen, F."MicroRNA‑185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator‑activated receptor β". Experimental and Therapeutic Medicine 21.4 (2021): 366.
Chicago
Yang, J., Deng, P., Qi, Y., Feng, X., Wen, H., Chen, F."MicroRNA‑185 inhibits the proliferation and migration of HaCaT keratinocytes by targeting peroxisome proliferator‑activated receptor β". Experimental and Therapeutic Medicine 21, no. 4 (2021): 366. https://doi.org/10.3892/etm.2021.9797
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