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The protective mechanism of action of plantamajoside on a rat model of acute spinal cord injury

  • Authors:
    • Hua Hu
    • Xiaofei Jian
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Jiangan, Wuhan, Hubei 430014, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 378
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    Published online on: February 19, 2021
       https://doi.org/10.3892/etm.2021.9809
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Abstract

Acute spinal cord injury (ASCI) is a severe traumatic disease of the central nervous system, characterized by a high incidence and high morbidity, for which there are no effective drug therapies in the clinic. A rat model of ASCI was established to study the effects of plantamajoside (PMS) treatment on the expression of apoptotic factors, including caspase‑3, caspase‑9, poly (ADP‑ribose) polymerase (PARP), Bax and Bcl‑2. The Allen's weight hit rat ASCI model was used for the present study, and the rats were treated with various concentrations of PMS. The behavior of rats was assessed using the Basso‑Beattle‑Bresnahan locomotor rating scale (BBB), the histopathologic changes of spinal cord tissue were observed by hematoxylin and eosin staining, the survival of neurons was assessed by TUNEL staining and the expression levels of apoptotic proteins such as caspase‑3, caspase‑9, PARP, Bcl‑2 and Bax was measured using western blot assays and RT‑qPCR. It was observed that PMS could reverse the decrease in the BBB score after ASCI, improve the morphological characteristics of the spinal cord, reduce the degree apoptosis and affect the expression of caspase‑3, caspase‑9, PARP, Bax and Bcl‑2 in a concentration dependent manner. In conclusion, PMS protected ASCI rats by inhibiting apoptosis; therefore PMS may be a potential candidate for ASCI therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Hu H and Jian X: The protective mechanism of action of plantamajoside on a rat model of acute spinal cord injury. Exp Ther Med 21: 378, 2021.
APA
Hu, H., & Jian, X. (2021). The protective mechanism of action of plantamajoside on a rat model of acute spinal cord injury. Experimental and Therapeutic Medicine, 21, 378. https://doi.org/10.3892/etm.2021.9809
MLA
Hu, H., Jian, X."The protective mechanism of action of plantamajoside on a rat model of acute spinal cord injury". Experimental and Therapeutic Medicine 21.4 (2021): 378.
Chicago
Hu, H., Jian, X."The protective mechanism of action of plantamajoside on a rat model of acute spinal cord injury". Experimental and Therapeutic Medicine 21, no. 4 (2021): 378. https://doi.org/10.3892/etm.2021.9809
Copy and paste a formatted citation
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Spandidos Publications style
Hu H and Jian X: The protective mechanism of action of plantamajoside on a rat model of acute spinal cord injury. Exp Ther Med 21: 378, 2021.
APA
Hu, H., & Jian, X. (2021). The protective mechanism of action of plantamajoside on a rat model of acute spinal cord injury. Experimental and Therapeutic Medicine, 21, 378. https://doi.org/10.3892/etm.2021.9809
MLA
Hu, H., Jian, X."The protective mechanism of action of plantamajoside on a rat model of acute spinal cord injury". Experimental and Therapeutic Medicine 21.4 (2021): 378.
Chicago
Hu, H., Jian, X."The protective mechanism of action of plantamajoside on a rat model of acute spinal cord injury". Experimental and Therapeutic Medicine 21, no. 4 (2021): 378. https://doi.org/10.3892/etm.2021.9809
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