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MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway

  • Authors:
    • Na Zhao
    • Wenhao Qin
    • Dongyue Wang
    • Anakarina González Raquel
    • Lichan Yuan
    • Yelin Mao
    • Changyan Ma
    • Zhongdang Xiao
    • Junqing Ma
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Orthodontics, The Affiliated Stomatology Hospital of Suzhou Vocational Health College, Suzhou, Jiangsu 215002, P.R. China, Department of Medical Genetics, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China, State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu 210096, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 379
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    Published online on: February 19, 2021
       https://doi.org/10.3892/etm.2021.9810
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Abstract

The neural crest is one of the key features of craniofacial development. MicroRNA‑1 (miR‑1) is a single‑stranded noncoding RNA that serves an important role in embryonic development. However, the function of miR‑1 in neural crest cells (NCCs) is unknown. Therefore, to evaluate the role of miR‑1 in NCC development, a miR‑1 mutant zebrafish was generated in the current study. Mouse NCCs were isolated from the first branchial arch of embryos at gestational day E9.5, and miR‑1 was silenced using a miR‑1 inhibitor. To the best of our knowledge, the present study was the first to report that homozygous zebrafish lacking miR‑1 exhibited developmental defects in NCC‑derived craniofacial bones, heart, melanocytes and iridophores. These defects may be caused by an increase in apoptosis of NCCs during their migration and differentiation in embryonic development. Moreover, the apoptosis analysis and western blotting results demonstrated that this effect was modulated via the mitochondrial apoptosis pathway, and miR‑1 inhibited NCC apoptosis by modulating this pathway. These results collectively suggested that miR‑1 in NCCs may be essential for craniofacial development.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao N, Qin W, Wang D, González Raquel A, Yuan L, Mao Y, Ma C, Xiao Z and Ma J: MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway. Exp Ther Med 21: 379, 2021.
APA
Zhao, N., Qin, W., Wang, D., González Raquel, A., Yuan, L., Mao, Y. ... Ma, J. (2021). MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway. Experimental and Therapeutic Medicine, 21, 379. https://doi.org/10.3892/etm.2021.9810
MLA
Zhao, N., Qin, W., Wang, D., González Raquel, A., Yuan, L., Mao, Y., Ma, C., Xiao, Z., Ma, J."MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway". Experimental and Therapeutic Medicine 21.4 (2021): 379.
Chicago
Zhao, N., Qin, W., Wang, D., González Raquel, A., Yuan, L., Mao, Y., Ma, C., Xiao, Z., Ma, J."MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway". Experimental and Therapeutic Medicine 21, no. 4 (2021): 379. https://doi.org/10.3892/etm.2021.9810
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao N, Qin W, Wang D, González Raquel A, Yuan L, Mao Y, Ma C, Xiao Z and Ma J: MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway. Exp Ther Med 21: 379, 2021.
APA
Zhao, N., Qin, W., Wang, D., González Raquel, A., Yuan, L., Mao, Y. ... Ma, J. (2021). MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway. Experimental and Therapeutic Medicine, 21, 379. https://doi.org/10.3892/etm.2021.9810
MLA
Zhao, N., Qin, W., Wang, D., González Raquel, A., Yuan, L., Mao, Y., Ma, C., Xiao, Z., Ma, J."MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway". Experimental and Therapeutic Medicine 21.4 (2021): 379.
Chicago
Zhao, N., Qin, W., Wang, D., González Raquel, A., Yuan, L., Mao, Y., Ma, C., Xiao, Z., Ma, J."MicroRNA‑1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway". Experimental and Therapeutic Medicine 21, no. 4 (2021): 379. https://doi.org/10.3892/etm.2021.9810
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