Oroxylin A attenuates IL‑1β‑induced inflammatory reaction via inhibiting the activation of the ERK and PI3K/AKT signaling pathways in osteoarthritis chondrocytes

Zhang,Yong; Weng,Qiuyan; Chen,Jianming; Li,Ming; Han,Jinming

doi:10.3892/etm.2021.9819

Oroxylin A attenuates IL‑1β‑induced inflammatory reaction via inhibiting the activation of the ERK and PI3K/AKT signaling pathways in osteoarthritis chondrocytes

Authors:
- Yong Zhang
- Qiuyan Weng
- Jianming Chen
- Ming Li
- Jinming Han
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Affiliations: Department of Trauma Orthopedics, Ningbo No. 6 Hospital, Ningbo, Zhejiang 315000, P.R. China, Department of Neurology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, Zhejiang 315000, P.R. China, Department of Spine, Ningbo No. 6 Hospital, Ningbo, Zhejiang 315000, P.R. China
Published online on: February 24, 2021 https://doi.org/10.3892/etm.2021.9819
Article Number: 388
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteoarthritis (OA) is characterized by degradation of the articular cartilage, synovium inflammation, subchondral bone sclerosis and osteophyte formation. OA is the most common degenerative joint disorder among the elderly population. In particular, currently available therapeutic strategies, such as non‑steroidal anti‑inflammatory drugs (NSAIDs) may cause severe side‑effects. Therefore, novel candidate targets for OA therapy are urgently needed. Oroxylin A (OrA) is a natural mono‑flavonoid that can be extracted from Scutellariae radix. The present study aimed to investigate the potential effects of OrA on interleukin (IL)‑1β‑induced chondrocytes inflammatory reactions. The current study performed quantitative PCR, western blotting and cell immunofluorescence to evaluate the effect of Oroxylin A in chondrocyte inflammation. The results demonstrated that OrA significantly attenuated the upregulation of inducible nitric oxide synthase and cyclooxygenase 2 by IL‑1β at both protein and mRNA levels. IL‑1β‑stimulated upregulation of matrix metalloproteinase (MMP)‑3 and MMP‑13 expression, in addition to disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)‑4 and ADAMTS‑5 expression, were all inhibited by OrA. Treatment with OrA significantly reversed the degradation of type II collagen and aggrecan by IL‑1β. Mechanistically, OrA suppressed the IL‑1β induced activation of ERK1/2 and PI3K/AKT signaling pathways. In conclusion, these findings suggest that OrA can serve as a potential therapeutic agent for the treatment of OA.

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