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Long non‑coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR‑146b‑5p/Robo1 axis

  • Authors:
    • Yu Jian
    • Qi Fan
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    Affiliations: Emergency Medical Department, Jingzhou Central Hospital, Jingzhou, Hubei 434020, P.R. China, Emergency Department, Jingzhou Central Hospital, Jingzhou, Hubei 434020, P.R. China
    Copyright: © Jian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 398
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    Published online on: February 24, 2021
       https://doi.org/10.3892/etm.2021.9829
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Abstract

Long non‑coding RNA (lncRNA) small nucleolar RNA host gene 7 (SNHG7) plays a crucial role in the progression of pancreatic cancer (PC). SNHG7 is upregulated in PC; therefore, the purpose of the present study was to investigate the role and underlying mechanism of SNHG7 on PC progression. In the present study, the mRNA expression levels of SNHG7, microRNA(miR)‑146b‑5p and roundabout homolog 1 (Robo1) were measured via reverse transcription‑quantitative PCR. Moreover, cell viability and apoptosis were assessed by MTT and flow cytometry assays, respectively. The ability of cells to migrate and invade was evaluated by Transwell assays. In addition, dual‑luciferase reporter, RNA immunoprecipitation and RNA pull‑down assays were conducted to assess the interaction between miR‑146b‑5p and SNHG7 or Robo1. The protein expression of Robo1 was measured via western blotting. Furthermore, mouse xenograft models were established to further investigate the effect of SNHG7 on PC progression in vivo. The results indicated that SNHG7 was highly expressed in PC tissues and cells. It was also found that SNHG7 was sponged by miR‑146b‑5p and that Robo1 was a target of miR‑146b‑5p. Moreover, it was demonstrated that SNHG7 knockdown inhibited cell proliferation, migration and invasion, as well as tumorigenesis and apoptosis of PC cells in vitro and in vivo by regulating miR‑146b‑5p. The results also suggested that miR‑146b‑5p overexpression inhibited the progression of PC cells by modulating Robo1. Furthermore, silencing of SNHG7 downregulated Robo1 expression by sponging miR‑146b‑5p. Collectively, the present results indicate that SNHG7 promotes PC progression by sponging miR‑146b‑5p and upregulating Robo1.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016.PubMed/NCBI View Article : Google Scholar

2 

Surveillance Epidemiology and End Results (SEER) Fact Sheets: Pancreas. Web site. http://seer.cancer.gov/. Accessed July 15, 2016.

3 

Aarnink A, Richard C, Truntzer C, Vincent J, Bengrine L, Vienot A, Borg C and Ghiringhelli F: Baseline splenic volume as a surrogate marker of FOLFIRINOX efficacy in advanced pancreatic carcinoma. Oncotarget. 9:25617–25629. 2018.PubMed/NCBI View Article : Google Scholar

4 

Paulson AS, Tran Cao HS, Tempero MA and Lowy AM: Therapeutic advances in pancreatic cancer. Gastroenterology. 144:1316–1326. 2013.PubMed/NCBI View Article : Google Scholar

5 

De Luca R, Blasi L, Alù M, Gristina V and Cicero G: Clinical efficacy of nab-paclitaxel in patients with metastatic pancreatic cancer. Drug Des Devel Ther. 12:1769–1775. 2018.PubMed/NCBI View Article : Google Scholar

6 

Yang G, Lu X and Yuan L: LncRNA: A link between RNA and cancer. Biochim Biophys Acta. 1839:1097–1109. 2014.PubMed/NCBI View Article : Google Scholar

7 

Tang Y, Cao G, Zhao G, Wang C and Qin Q: LncRNA differentiation antagonizing non-protein coding RNA promotes proliferation and invasion through regulating miR-135a/NLRP37 axis in pancreatic cancer. Invest New Drugs. 38:714–721. 2019.PubMed/NCBI View Article : Google Scholar

8 

Peng W and Jiang A: Long noncoding RNA CCDC26 as a potential predictor biomarker contributes to tumorigenesis in pancreatic cancer. Biomed Pharmacother. 83:712–717. 2016.PubMed/NCBI View Article : Google Scholar

9 

Zhang X, Feng W, Zhang J, Ge L, Zhang Y, Jiang X, Peng W, Wang D, Gong A and Xu M: Long non-coding RNA PVT1 promotes epithelial-mesenchymal transition via the TGF-β/Smad pathway in pancreatic cancer cells. Oncol Rep. 40:1093–1102. 2018.PubMed/NCBI View Article : Google Scholar

10 

Zhang M, Zhao Y, Zhang Y, Wang D, Gu S, Feng W, Peng W, Gong A and Xu M: LncRNA UCA1 promotes migration and invasion in pancreatic cancer cells via the hippo pathway. Biochim Biophys Acta Mol Basis Dis. 1864:1770–1782. 2018.PubMed/NCBI View Article : Google Scholar

11 

Gao H, Gong N, Ma Z, Miao X, Chen J, Cao Y and Zhang G: LncRNA ZEB2-AS1 promotes pancreatic cancer cell growth and invasion through regulating the miR-204/HMGB1 axis. Int J Biol Macromol. 116:545–551. 2018.PubMed/NCBI View Article : Google Scholar

12 

She K, Huang J, Zhou H, Huang T, Chen G and He J: lncRNA-SNHG7 promotes the proliferation, migration and invasion and inhibits apoptosis of lung cancer cells by enhancing the FAIM2 expression. Oncol Rep. 36:2673–2680. 2016.PubMed/NCBI View Article : Google Scholar

13 

Wang MW, Liu J, Liu Q, Xu QH, Li TF, Jin S and Xia TS: LncRNA SNHG7 promotes the proliferation and inhibits apoptosis of gastric cancer cells by repressing the P15 and P16 expression. Eur Rev Med Pharmacol Sci. 21:4613–4622. 2017.PubMed/NCBI

14 

Ren J, Yang Y, Xue J, Xi Z, Hu L, Pan SJ and Sun Q: Long noncoding RNA SNHG7 promotes the progression and growth of glioblastoma via inhibition of miR-5095. Biochem Biophys Res Commun. 496:712–718. 2018.PubMed/NCBI View Article : Google Scholar

15 

Cheng D, Fan J, Ma Y, Zhou Y, Qin K, Shi M and Yang J: LncRNA SNHG7 promotes pancreatic cancer proliferation through ID4 by sponging miR-342-3p. Cell Biosci. 9(28)2019.PubMed/NCBI View Article : Google Scholar

16 

Esteller M: Non-coding RNAs in human disease. Nat Rev Genet. 12:861–874. 2011.PubMed/NCBI View Article : Google Scholar

17 

Zhang Z, Che X, Yang N, Bai Z, Wu Y, Zhao L and Pei H: MiR-135b-5p Promotes migration, invasion and EMT of pancreatic cancer cells by targeting NR3C2. Biomed Pharmacother. 96:1341–1348. 2017.PubMed/NCBI View Article : Google Scholar

18 

Cai H, Yao J, An Y, Chen X, Chen W, Wu D, Luo B, Yang Y, Jiang Y, Sun D and He X: LncRNA HOTAIR acts a competing endogenous RNA to control the expression of notch3 via sponging miR-613 in pancreatic cancer. Oncotarget. 8:32905–32917. 2017.PubMed/NCBI View Article : Google Scholar

19 

Wang J, Guo XJ, Ding YM and Jiang JX: MiR-1181 inhibits invasion and proliferation via STAT3 in pancreatic cancer. World J Gastroenterol. 23:1594–1601. 2017.PubMed/NCBI View Article : Google Scholar

20 

Lin F, Wang X, Jie Z, Hong X, Li X, Wang M and Yu Y: Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by targeting MMP16. J Huazhong Univ Sci Technolog Med Sci. 31(509)2011.PubMed/NCBI View Article : Google Scholar

21 

He H, Hao SJ, Yao L, Yang F, Di Y, Li J, Jiang YJ, Jin C and Fu DL: MicroRNA-218 inhibits cell invasion and migration of pancreatic cancer via regulating ROBO1. Cancer Biol Ther. 15:1333–1339. 2014.PubMed/NCBI View Article : Google Scholar

22 

Cuccurullo V and Mansi L: AJCC cancer staging handbook: From the AJCC cancer staging manual (7th edition). Eur J Nucl Med Mol Imaging. 38(408)2011.

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

24 

Harden VA and Hannaway C: National Institutes of Health (NIH). In: Encyclopedia of Life Sciences. New York, John Wiley & Sons, Ltd, 2001.

25 

Schober P, Boer C and Schwarte LA: Correlation coefficients: Appropriate use and interpretation. Anesth Analg. 126:1763–1768. 2018.PubMed/NCBI View Article : Google Scholar

26 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jemal A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018.PubMed/NCBI View Article : Google Scholar

27 

Xu W, Chang J, Du X and Hou J: Long non-coding RNA PCAT-1 contributes to tumorigenesis by regulating FSCN1 via miR-145-5p in prostate cancer. Biomed Pharmacother. 95:1112–1118. 2017.PubMed/NCBI View Article : Google Scholar

28 

Huo X and Wang H, Huo B, Wang L, Yang K, Wang J, Wang L and Wang H: FTX contributes to cell proliferation and migration in lung adenocarcinoma via targeting miR-335-5p/NUCB2 axis. Cancer Cell Int. 20(89)2020.PubMed/NCBI View Article : Google Scholar

29 

Sun J, Zhang P, Yin T, Zhang F and Wang W: Upregulation of LncRNA PVT1 facilitates pancreatic ductal adenocarcinoma cell progression and glycolysis by regulating MiR-519d-3p and HIF-1A. J Cancer. 11:2572–2579. 2020.PubMed/NCBI View Article : Google Scholar

30 

Guo X, Zhang Y, Liu L, Yang W and Zhang Q: HNF1A-AS1 regulates cell migration, invasion and glycolysis via modulating miR-124/MYO6 in colorectal cancer cells. Onco Targets Ther. 13:1507–1518. 2020.PubMed/NCBI View Article : Google Scholar

31 

Li H, Yao G, Zhai J, Hu D and Fan Y: LncRNA FTX promotes proliferation and invasion of gastric cancer via miR-144/ZFXAxis. Onco Targets Ther. 12:11701–11713. 2019.PubMed/NCBI View Article : Google Scholar

32 

Feng Y, Gao L, Cui G and Cao Y: LncRNA NEAT1 facilitates pancreatic cancer growth and metastasis through stabilizing ELF3 mRNA. Am J Cancer Res. 10:237–248. 2020.PubMed/NCBI

33 

Zhang C, Zhu B, Li XB, Cao YQ, Yang JC, Li X, Liu YX and Wang YB: Long non-coding RNA SNHG7 promotes migration and invasion of melanoma via upregulating SOX4. Eur Rev Med Pharmacol Sci. 23:4828–4834. 2019.PubMed/NCBI View Article : Google Scholar

34 

Wang YH, Huo BL, Li C, Ma G and Cao W: Knockdown of long noncoding RNA SNHG7 inhibits the proliferation and promotes apoptosis of thyroid cancer cells by downregulating BDNF. Eur Rev Med Pharmacol Sci. 23:4815–4821. 2019.PubMed/NCBI View Article : Google Scholar

35 

Xu C, Zhou J, Wang Y, Wang A, Su L, Liu S and Kang X: Inhibition of malignant human bladder cancer phenotypes through the down-regulation of the long non-coding RNA SNHG7. J Cancer. 10:539–546. 2019.PubMed/NCBI View Article : Google Scholar

36 

Luo X, Song Y, Tang L, Sun DH and Ji DG: LncRNA SNHG7 promotes development of breast cancer by regulating microRNA-186. Eur Rev Med Pharmacol Sci. 22:7788–7797. 2018.PubMed/NCBI View Article : Google Scholar

37 

Sun X, Huang T, Liu Z, Sun M and Luo S: LncRNA SNHG7 contributes to tumorigenesis and progression in breast cancer by interacting with miR-34a through EMT initiation and the notch-1 pathway. Eur J Pharmacol. 856(172407)2019.PubMed/NCBI View Article : Google Scholar

38 

Chen W, Ye L, Wen D and Chen F: MiR-490-5p inhibits hepatocellular carcinoma cell proliferation, migration and invasion by directly regulating ROBO1. Pathol Oncol Res. 25:1–9. 2019.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Jian Y and Fan Q: Long non‑coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR‑146b‑5p/Robo1 axis. Exp Ther Med 21: 398, 2021.
APA
Jian, Y., & Fan, Q. (2021). Long non‑coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR‑146b‑5p/Robo1 axis. Experimental and Therapeutic Medicine, 21, 398. https://doi.org/10.3892/etm.2021.9829
MLA
Jian, Y., Fan, Q."Long non‑coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR‑146b‑5p/Robo1 axis". Experimental and Therapeutic Medicine 21.4 (2021): 398.
Chicago
Jian, Y., Fan, Q."Long non‑coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR‑146b‑5p/Robo1 axis". Experimental and Therapeutic Medicine 21, no. 4 (2021): 398. https://doi.org/10.3892/etm.2021.9829
Copy and paste a formatted citation
x
Spandidos Publications style
Jian Y and Fan Q: Long non‑coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR‑146b‑5p/Robo1 axis. Exp Ther Med 21: 398, 2021.
APA
Jian, Y., & Fan, Q. (2021). Long non‑coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR‑146b‑5p/Robo1 axis. Experimental and Therapeutic Medicine, 21, 398. https://doi.org/10.3892/etm.2021.9829
MLA
Jian, Y., Fan, Q."Long non‑coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR‑146b‑5p/Robo1 axis". Experimental and Therapeutic Medicine 21.4 (2021): 398.
Chicago
Jian, Y., Fan, Q."Long non‑coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR‑146b‑5p/Robo1 axis". Experimental and Therapeutic Medicine 21, no. 4 (2021): 398. https://doi.org/10.3892/etm.2021.9829
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