Intracerebral hemorrhage alters α2δ1 and thrombospondin expression in rats

Wang,Bingqian; Li,Xiaopeng; Yu,Ning; Yang,Liang; Nan,Chengrui; Guo,Lisi; Zhao,Zongmao

doi:10.3892/etm.2022.11256

Intracerebral hemorrhage alters α2δ1 and thrombospondin expression in rats

Authors:
- Bingqian Wang
- Xiaopeng Li
- Ning Yu
- Liang Yang
- Chengrui Nan
- Lisi Guo
- Zongmao Zhao
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Affiliations: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China
Published online on: March 15, 2022 https://doi.org/10.3892/etm.2022.11256
Article Number: 327
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Calcium voltage‑gated channel auxiliary subunit (α2δ1) is a non‑essential subunit of the voltage‑gated calcium channel complex and is ubiquitously expressed in a number of tissues, including the brain. Thrombospondin (TSP)1 and TSP2 are extracellular matrix proteins and belong to the multi‑domain glycoprotein family of macromolecular oligomers. TSP1/2 and α2δ1 promote synapse formation and functional recovery following cerebral infarction. However, to the best of our knowledge, the expression levels of α2δ1 and TSP1/2 in brain tissues, and the effects of intracerebral hemorrhage (ICH) on these levels have not yet been elucidated. The present study established a rat model of hemorrhage induced by injecting collagenase IV into the striatum to determine the changes in α2δ1 and TSP1/2 expression following ICH. The protein expression levels of α2δ1 and TSP1 in the striatum after hemorrhage were significantly increased on day 5 and returned to baseline levels on day 21; however, the protein expression levels of TSP2 were decreased on day 5, whereas they increased on day 14, subsequently returning to baseline levels. In addition, using proteomics analysis of tissues from the sham group (saline injection) and at 24 h post‑ICH, it was found that both α2δ1 and TSP1 interacted with neural EGFL like 2. Taken together, these findings suggested that the expression levels of α2δ1 and TSP1/2 were altered in brain tissues in response to ICH.

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