Altered small non‑coding RNA expression profiles of extracellular vesicles in the prostatic fluid of patients with chronic pelvic pain syndrome

Ouyang,Bin; Ouyang,Bin; Ouyang,Bin; Ouyang,Bin; Ouyang,Bin; Han,Dayu; Han,Dayu; Han,Dayu; Han,Dayu; Han,Dayu; Guo,Zexin; Guo,Zexin; Guo,Zexin; Guo,Zexin; Guo,Zexin; Deng,Junhong; Deng,Junhong; Deng,Junhong; Deng,Junhong; Deng,Junhong; Li,Weilong; Li,Weilong; Li,Weilong; Li,Weilong; Li,Weilong; Huang,Liangliang; Huang,Liangliang; Huang,Liangliang; Huang,Liangliang; Huang,Liangliang; Liu,Jianming; Liu,Jianming; Liu,Jianming; Liu,Jianming; Liu,Jianming; Cai,Zhouda; Cai,Zhouda; Cai,Zhouda; Cai,Zhouda; Cai,Zhouda; Bian,Jun; Bian,Jun; Bian,Jun; Bian,Jun; Bian,Jun; Huang,Shaoming; Huang,Shaoming; Huang,Shaoming; Huang,Shaoming; Huang,Shaoming

doi:10.3892/etm.2022.11310

Altered small non‑coding RNA expression profiles of extracellular vesicles in the prostatic fluid of patients with chronic pelvic pain syndrome

Authors:
- Bin Ouyang
- Dayu Han
- Zexin Guo
- Junhong Deng
- Weilong Li
- Liangliang Huang
- Jianming Liu
- Zhouda Cai
- Jun Bian
- Shaoming Huang
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Affiliations: Department of Andrology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China, Department of Andrology, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Reproductive Centre, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Urology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China, Department of Urology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510700, P.R. China, Department of Andrology, Ganzhou Municipal Hospital, Ganzhou, Jiangxi 341000, P.R. China
Published online on: April 8, 2022 https://doi.org/10.3892/etm.2022.11310
Article Number: 382
Copyright: © Ouyang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chronic pelvic pain syndrome (CPPS) and chronic prostatitis (CP) is difficult to distinguish from each other, herein termed CP/CPPS. The present study aimed at gaining further insight into the change in extracellular vesicles (EVs) in the prostatic fluid of males with CPPS. From December 2019 to November 2020, after clinical screening, 24 patients with CPPS without obvious urinary symptoms and 13 healthy male participants were included. EVs were isolated from expressed prostatic secretion (EPS) of all subjects. The small non‑coding ribonucleic acid (sncRNA) expression of EVs was sequenced, analyzed, and validated by quantitative real‑time polymerase chain reaction (qPCR) assays. The results showed that numerous sncRNAs were differentially expressed between the patients and healthy participants. Further qPCR assays validated that several chronic pain‑related miRNAs, including miR‑204‑5p, let‑7d‑3p, let‑7b‑3p, let‑7c‑3p, miR‑146a‑5p, and miR‑320a‑5p, were differentially expressed. Series sncRNAs including several chronic pain‑related miRNAs were altered in EVs in prostatic fluid of patients with CPPS, which may serve as diagnostic markers for CPPS.

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