Overexpression of long non‑coding RNA NEAT1 enhances cell viability and inhibits apoptosis in recurrent spontaneous abortion by targeting the miR‑125b/BCL‑2 axis

  • Authors:
    • Xiaodan Liu
    • Li Su
    • Bingnv Xu
    • Jing Lei
    • Hongjie Zhang
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  • Published online on: April 13, 2022     https://doi.org/10.3892/etm.2022.11319
  • Article Number: 392
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Abstract

The current study aimed to investigate the function of the long non‑coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) in the pathogenesis of recurrent spontaneous abortion (RSA) and to examine its potential mechanism. The expression of NEAT1, microRNA (miR)‑125b and Bcl‑2 in the villi of patients with RSAs and women with normal pregnancies was measured by reverse transcription‑quantitative PCR. Cell viability was detected by the MTT assay and cell apoptosis was evaluated by flow cytometry. A dual‑luciferase reporter assay was performed to verify the associations between NEAT1 and miR‑125b. The protein expression of Bcl‑2 was detected by western blot analysis. In the present study, the expression of NEAT1 and Bcl‑2 was reduced and that of miR‑125b was increased in clinical samples of villus tissues from patients with RSAs. In vitro, overexpression of NEAT1 enhanced the viability and suppressed the apoptosis of JEG‑3 cells. It was demonstrated that miR‑125b acts as a molecular sponge of NEAT1 and its expression was negatively regulated by NEAT1. miR‑125b overexpression reduced the viability and promoted the apoptosis of JEG‑3 cells. The expression of BCL‑2, a target gene of miR‑125b, was inversely correlated with that of miR‑125b. Overexpression of miR‑125b and inhibition of BCL‑2 partially reversed the effect of NEAT1 overexpression on the viability and apoptosis of JEG‑3 cells. Collectively, it was demonstrated that the NEAT1/miR‑125b/BCL‑2 axis plays a pivotal role in regulating the viability and apoptosis of JEG‑3 cells. The findings of the present study offer new insights into the pathogenesis of RSA and may provide information on RSA treatment.
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June-2022
Volume 23 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu X, Su L, Xu B, Lei J and Zhang H: Overexpression of long non‑coding RNA NEAT1 enhances cell viability and inhibits apoptosis in recurrent spontaneous abortion by targeting the miR‑125b/BCL‑2 axis. Exp Ther Med 23: 392, 2022
APA
Liu, X., Su, L., Xu, B., Lei, J., & Zhang, H. (2022). Overexpression of long non‑coding RNA NEAT1 enhances cell viability and inhibits apoptosis in recurrent spontaneous abortion by targeting the miR‑125b/BCL‑2 axis. Experimental and Therapeutic Medicine, 23, 392. https://doi.org/10.3892/etm.2022.11319
MLA
Liu, X., Su, L., Xu, B., Lei, J., Zhang, H."Overexpression of long non‑coding RNA NEAT1 enhances cell viability and inhibits apoptosis in recurrent spontaneous abortion by targeting the miR‑125b/BCL‑2 axis". Experimental and Therapeutic Medicine 23.6 (2022): 392.
Chicago
Liu, X., Su, L., Xu, B., Lei, J., Zhang, H."Overexpression of long non‑coding RNA NEAT1 enhances cell viability and inhibits apoptosis in recurrent spontaneous abortion by targeting the miR‑125b/BCL‑2 axis". Experimental and Therapeutic Medicine 23, no. 6 (2022): 392. https://doi.org/10.3892/etm.2022.11319