Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
June-2022 Volume 23 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2022 Volume 23 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Inonotsuoxide B suppresses hepatic stellate cell activation and proliferation via the PI3K/AKT and ERK1/2 pathway

  • Authors:
    • Juan Jin
    • Hui Yang
    • Lili Hu
    • Yinghong Wang
    • Wenyong Wu
    • Chengmu Hu
    • Kun Wu
    • Zehua Wu
    • Wenming Cheng
    • Yan Huang
  • View Affiliations / Copyright

    Affiliations: Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230032, P.R. China, Department of Pharmacy, Division of Life Sciences and Medicine, Anhui Provincial Cancer Hospital, The First Affiliated Hospital of University of Science and Technology of China, Hefei, Anhui 230001, P.R. China, Department of General Surgery, Anhui No. 2 Provincial People's Hospital, Hefei, Anhui 230041, P.R. China
  • Article Number: 417
    |
    Published online on: April 28, 2022
       https://doi.org/10.3892/etm.2022.11344
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hepatic stellate cells (HSCs) serve a pivotal role in the formation and degradation of the extracellular matrix during liver fibrosis. Inonotsuoxide B is a tetracyclic triterpenoid that can be extracted from Inonotus obliquus and has been previously reported to inhibit the growth of liver and gastric cancer cells. However, its effect on liver fibrosis remain poorly understood. Therefore, in the present study, the potential antiproliferative effects of inonotsuoxide B on HSCs was investigated. Initially, cells were divided into the following five groups: Control; platelet‑derived growth factor (PDGF)‑BB (10 ng/ml); and PDGF‑BB + inonotsuoxide B (5, 10 and 20 µg/ml) groups. Inonotsuoxide B treatment (5, 10 and 20 µg/ml) was revealed to reverse PDGF‑BB‑induced HSC proliferation. Furthermore, the protein expression of α‑smooth‑muscle actin (α‑SMA) and type I collagen was significantly decreased in the inonotsuoxide B (10 and 20 µg/ml) groups compared with the PDGF‑BB group. Inonotsuoxide B (5, 10 and 20 µg/ml) was also revealed to suppress PDGF‑BB‑induced α‑SMA mRNA expression and activation of the PI3K/AKT and ERK signaling pathways in HSCs. These findings suggest that inonotsuoxide B suppresses the proliferation and activation of HSCs by inhibiting the PI3K/AKT and ERK1/2 signaling pathways.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Ping J, Li JT, Liao ZX, Shang L and Wang H: Indole-3-carbinol inhibits hepatic stellate cells proliferation by blocking NADPH oxidase/reactive oxygen species/p38 MAPK pathway. Eur J Pharmacol. 650:656–662. 2011.PubMed/NCBI View Article : Google Scholar

2 

Tsai MK, Lin YL and Huang YT: Differential inhibitory effects of salvianolic acids on activation of rat hepatic stellate cells by platelet-derived growth factor. Planta Med. 77:1495–1503. 2011.PubMed/NCBI View Article : Google Scholar

3 

Wang YH, Sun YC, Zuo LQ, Wang YN and Huang Y: ASIC1a promotes high glucose and PDGF-induced hepatic stellate cell activation by inducing autophagy through CaMKKβ/ERK signaling pathway. Toxicol Lett. 300:1–9. 2018.PubMed/NCBI View Article : Google Scholar

4 

Shuping You, Jun Zhao and Long Ma: Effects of total glycosides of cistanche deserticola on the proliferation of hepatic stellate cells induced by platelet-derived growth factor. Chin J Pharmacol. 32:1231–1235. 2016.

5 

Choi JH, Hwang YP, Park BH, Choi CY, Chung YC and Jeong HG: Anthocyanins isolated from the purple-fleshed sweet potato attenuate the proliferation of hepatic stellate cells by blocking the PDGF receptor. Environ Toxicol Pharmacol. 31:212–219. 2011.PubMed/NCBI View Article : Google Scholar

6 

Youn MJ, Kim JK, Park SY, Kim Y, Kim SJ, Lee JS, Chai KY, Kim HJ, Cui MX, So HS, et al: Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells. World J Gastroenterol. 14:511–517. 2008.PubMed/NCBI View Article : Google Scholar

7 

Duru KC, Kovaleva EG, Danilova IG and van der Bijl P: The pharmacological potential and possible molecular mechanisms of action of Inonotus obliquus from preclinical studies. Phytother Res. 33:1966–1980. 2019.PubMed/NCBI View Article : Google Scholar

8 

Kun WU, Wenming Cheng, Chunru Li, et al: Screening and chemical constituents of anti-tumor active parts of inonotus obliquus. J Anhui Medical University. 51:1468–1472. 2016.

9 

Huang Y, Li X, Wang Y, Wang H, Huang C and Li J: Endoplasmic reticulum stress-induced hepatic stellate cell apoptosis through calcium-mediated JNK/P38 MAPK and Calpain/Caspase-12 pathways. Mol Cell Biochem. 394:1–12. 2014.PubMed/NCBI View Article : Google Scholar

10 

Foo NP, Lin SH, Lee YH, Wu MJ and Wang YJ: α-Lipoic acid inhibits liver fibrosis through the attenuation of ROS-triggered signaling in hepatic stellate cells activated by PDGF and TGF-β. Toxicology. 282:39–46. 2011.PubMed/NCBI View Article : Google Scholar

11 

Huang Y, Leng TD, Inoue K, Yang T, Liu M, Horgen FD, Fleig A, Li J and Xiong ZG: TRPM7 channels play a role in high glucose-induced endoplasmic reticulum stress and neuronal cell apoptosis. J Biol Chem. 293:14393–14406. 2018.PubMed/NCBI View Article : Google Scholar

12 

Wang H, Wang YH, Yang F, Li XF, Tian YY, Ni MM, Zuo LQ, Meng XM and Huang Y: Effect of Acid-sensing ion Channel 1a on the process of liver fibrosis under hyperglycemia. Biochem Biophys Res Commun. 468:758–765. 2015.PubMed/NCBI View Article : Google Scholar

13 

Li J, Li X, Xu W, Wang S, Hu Z, Zhang Q, Deng X, Wang J, Zhang J and Guo C: Antifibrotic effects of luteolin on hepatic stellate cells and liver fibrosis by targeting AKT/mTOR/p70S6K and TGFβ/Smad signalling pathways. Liver Int. 35:1222–1233. 2015.PubMed/NCBI View Article : Google Scholar

14 

Tsai TH, Shih SC, Ho TC, Ma HI, Liu MY, Chen SL and Tsao YP: Pigment epithelium-derived factor 34-mer peptide prevents liver fibrosis and hepatic stellate cell activation through down-regulation of the PDGF receptor. PLoS One. 9(e95443)2014.PubMed/NCBI View Article : Google Scholar

15 

Yujie Zhang, Yutao Hong and Lei Jie: Effects of melatonin on proliferation of HSC-T6 cells induced by PDGF-BB and its mechanism. J Anhui Med University. 53:12–17. 2018.

16 

You SP, Zhao J, Ma L, Tudimat M, Zhang SL and Liu T: Preventive effects of phenylethanol glycosides from Cistanche tubulosa on bovine serum albumin-induced hepatic fibrosis in rats. Daru. 23(52)2015.PubMed/NCBI View Article : Google Scholar

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expres-sion data using real-time quantitative PCR and the 2(-Delta DeltaC(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

18 

Mazumder AG, Kumari S and Singh D: Anticonvulsant action of a selective phosphatidylinositol-3-kinase inhibitor LY294002 in pentylenetetrazole-mediated convulsions in zebrafish. Epilepsy Res. 157(106207)2019.PubMed/NCBI View Article : Google Scholar

19 

Cole GW Jr, Alleva AM, Zuo JT, Sehgal SS, Yeow WS, Schrump DS and Nguyen DM: Suppression of pro-metastasis phenotypes expression in malignant pleural mesothelioma by the PI3K inhibitor LY294002 or the MEK inhibitor UO126. Anticancer Res. 26:809–821. 2006.PubMed/NCBI

20 

Lee UE and Friedman SL: Mechanisms of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol. 25:195–206. 2011.PubMed/NCBI View Article : Google Scholar

21 

Bataller R and Brenner DA: Hepatic stellate cells as a target for the treatment of liver fibrosis. Semin Liver Dis. 21:437–452. 2001.PubMed/NCBI View Article : Google Scholar

22 

Wang Y, Gao J, Zhang D, Zhang J, Ma J and Jiang H: New insights into the antifibrotic effects of sorafenib on hepatic stellate cells and liver fibrosis. J Hepatol. 53:132–144. 2010.PubMed/NCBI View Article : Google Scholar

23 

Balandaykin ME and Zmitrovich IV: Review on chaga medicinal mushroom, inonotus obliquus (Higher Basidiomycetes): Realm of medicinal applications and approaches on estimating its resource potential. Int J Med Mushrooms. 17:95–104. 2015.PubMed/NCBI View Article : Google Scholar

24 

Glamočlija J, Ćirić A, Nikolić M, Fernandes Â, Barros L, Calhelha RC, Ferreira IC, Soković M and van Griensven LJ: Chemical characterization and biological activity of Chaga (Inonotus obliquus), a medicinal ‘mushroom’. J Ethnopharmacol. 162:323–332. 2015.PubMed/NCBI View Article : Google Scholar

25 

Wang GJ, Huang YJ, Chen DH and Lin YL: Ganoderma lucidum extract attenuates the proliferation of hepatic stellate cells by blocking the PDGF receptor. Phytother Res. 23:833–839. 2009.PubMed/NCBI View Article : Google Scholar

26 

Jiang M, Wu YL, Li X, Zhang Y, Xia KL, Cui BW, Lian LH and Nan JX: Oligomeric proanthocyanidin derived from grape seeds inhibited NF-κB signaling in activated HSC: Involvement of JNK/ERK MAPK and PI3K/Akt pathways. Biomed Pharmacother. 93:674–680. 2017.PubMed/NCBI View Article : Google Scholar

27 

Kao YH, Chen PH, Wu TY, Lin YC, Tsai MS, Lee PH, Tai TS, Chang HR and Sun CK: Lipopolysaccharides induce Smad2 phosphorylation through PI3K/Akt and MAPK cascades in HSC-T6 hepatic stellate cells. Life Sci. 184:37–46. 2017.PubMed/NCBI View Article : Google Scholar

28 

Fang L, Zhan S, Huang C, Cheng X, Lv X, Si H and Li J: TRPM7 channel regulates PDGF-BB-induced proliferation of hepatic stellate cells via PI3K and ERK pathways. Toxicol Appl Pharmacol. 27:713–725. 2013.PubMed/NCBI View Article : Google Scholar

29 

He W, Shi F, Zhou ZW, Li B, Zhang K, Zhang X, Ouyang C, Zhou SF and Zhu X: A bioinformatic and mechanistic study elicits the antifibrotic effect of ursolic acid through the attenuation of oxidative stress with the involvement of ERK, PI3K/Akt, and p38 MAPK signaling pathways in human hepatic stellate cells and rat liver. Drug Des Devel Ther. 9:3989–4104. 2015.PubMed/NCBI View Article : Google Scholar

30 

Li Jinxing, Zhu Xianli and Li Yu: Rats with diffuse brain injury block the ERK pathway and down-regulate the expression of MMP-9 mRNA in brain tissue. Chin J Clin Neurosurg. 11:288–291. 2006.

31 

You Linlin, Dai Lili, et al: Inhibitory effect of Danshensu on hepatic stellate cells stimulated by PDGF-BB. J Third Military Med University. 33:1610–1614. 2011.

32 

Xu DD, Li XF, Li YH, Liu YH, Huang C, Meng XM and Li J: TIPE2 attenuates liver fibrosis by reversing the activated hepatic stellate cells. Biochem Biophys Res Commun. 498:199–206. 2018.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jin J, Yang H, Hu L, Wang Y, Wu W, Hu C, Wu K, Wu Z, Cheng W, Huang Y, Huang Y, et al: Inonotsuoxide B suppresses hepatic stellate cell activation and proliferation via the PI3K/AKT and ERK1/2 pathway. Exp Ther Med 23: 417, 2022.
APA
Jin, J., Yang, H., Hu, L., Wang, Y., Wu, W., Hu, C. ... Huang, Y. (2022). Inonotsuoxide B suppresses hepatic stellate cell activation and proliferation via the PI3K/AKT and ERK1/2 pathway. Experimental and Therapeutic Medicine, 23, 417. https://doi.org/10.3892/etm.2022.11344
MLA
Jin, J., Yang, H., Hu, L., Wang, Y., Wu, W., Hu, C., Wu, K., Wu, Z., Cheng, W., Huang, Y."Inonotsuoxide B suppresses hepatic stellate cell activation and proliferation via the PI3K/AKT and ERK1/2 pathway". Experimental and Therapeutic Medicine 23.6 (2022): 417.
Chicago
Jin, J., Yang, H., Hu, L., Wang, Y., Wu, W., Hu, C., Wu, K., Wu, Z., Cheng, W., Huang, Y."Inonotsuoxide B suppresses hepatic stellate cell activation and proliferation via the PI3K/AKT and ERK1/2 pathway". Experimental and Therapeutic Medicine 23, no. 6 (2022): 417. https://doi.org/10.3892/etm.2022.11344
Copy and paste a formatted citation
x
Spandidos Publications style
Jin J, Yang H, Hu L, Wang Y, Wu W, Hu C, Wu K, Wu Z, Cheng W, Huang Y, Huang Y, et al: Inonotsuoxide B suppresses hepatic stellate cell activation and proliferation via the PI3K/AKT and ERK1/2 pathway. Exp Ther Med 23: 417, 2022.
APA
Jin, J., Yang, H., Hu, L., Wang, Y., Wu, W., Hu, C. ... Huang, Y. (2022). Inonotsuoxide B suppresses hepatic stellate cell activation and proliferation via the PI3K/AKT and ERK1/2 pathway. Experimental and Therapeutic Medicine, 23, 417. https://doi.org/10.3892/etm.2022.11344
MLA
Jin, J., Yang, H., Hu, L., Wang, Y., Wu, W., Hu, C., Wu, K., Wu, Z., Cheng, W., Huang, Y."Inonotsuoxide B suppresses hepatic stellate cell activation and proliferation via the PI3K/AKT and ERK1/2 pathway". Experimental and Therapeutic Medicine 23.6 (2022): 417.
Chicago
Jin, J., Yang, H., Hu, L., Wang, Y., Wu, W., Hu, C., Wu, K., Wu, Z., Cheng, W., Huang, Y."Inonotsuoxide B suppresses hepatic stellate cell activation and proliferation via the PI3K/AKT and ERK1/2 pathway". Experimental and Therapeutic Medicine 23, no. 6 (2022): 417. https://doi.org/10.3892/etm.2022.11344
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team