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Lenalidomide improves H2O2‑induced PC12 cell injury by blocking the Notch signaling pathway

  • Authors:
    • Zheng Lv
    • Shao Yin
    • Ziguan Cheng
    • Kekai Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610032, P.R. China, Anorectal Department, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610032, P.R. China
    Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 421
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    Published online on: April 29, 2022
       https://doi.org/10.3892/etm.2022.11348
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Abstract

Lenalidomide (LEN) has been reported to exert antitumor, anti‑inflammatory, anti‑angiogenic and immunomodulatory activities. However, the effects of LEN in spinal cord injury (SCI) are yet to be fully elucidated. The present study was conducted to identify whether LEN has a healing effect on SCI and to determine the underlying mechanism of action. The viability of H2O2‑stimulated PC12 cells was detected using a Cell Counting Kit‑8 assay. The viability of PC12 cells treated with LEN was examined with an MTT assay. The level of lactate dehydrogenase (LDH) was measured using an LDH assay kit, while the levels of the oxidative stress‑related factors malondialdehyde, superoxide dismutase, glutathione peroxidase and catalase in PC12 cells were determined using commercial kits. Oxidative stress‑related proteins were examined via western blotting. PC12 cell apoptosis was observed using a TUNEL assay, while apoptosis markers and Notch signaling‑related proteins were examined via western blotting. The results of the present study demonstrated that H2O2 decreased PC12 cell viability in a dose‑dependent manner. However, treatment with LEN significantly improved the viability of H2O2‑stimulated PC12 cells and alleviated H2O2‑induced cytotoxic injury. Additionally, LEN treatment inhibited the oxidative stress and apoptosis induced by H2O2 in PC12 cells. Notch signaling‑related protein expression was downregulated after LEN treatment in H2O2‑stimulated PC12 cells. In addition, a Notch agonist reversed the effects of LEN on H2O2‑induced oxidative stress and PC12 cell apoptosis. In summary, it was demonstrated that LEN alleviated the H2O2‑induced injury of PC12 cells by blocking the Notch signaling pathway, suggesting the value of applying LEN to the treatment of SCI.
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1 

Eckert MJ and Martin MJ: Trauma: Spinal cord injury. Surg Clin North Am. 97:1031–1045. 2017.PubMed/NCBI View Article : Google Scholar

2 

Hamid R, Averbeck MA, Chiang H, Garcia A, Al Mousa RT, Oh SJ, Patel A, Plata M and Del Popolo G: Epidemiology and pathophysiology of neurogenic bladder after spinal cord injury. World J Urol. 36:1517–1527. 2018.PubMed/NCBI View Article : Google Scholar

3 

Witiw CD and Fehlings MG: Acute spinal cord injury. J Spinal Disord Tech. 28:202–210. 2015.PubMed/NCBI View Article : Google Scholar

4 

Kumar R, Lim J, Mekary RA, Rattani A, Dewan MC, Sharif SY, Osorio-Fonseca E and Park KB: Traumatic spinal injury: Global epidemiology and worldwide volume. World Neurosurg. 113:e345–e363. 2018.PubMed/NCBI View Article : Google Scholar

5 

Kjell J and Olson L: Rat models of spinal cord injury: From pathology to potential therapies. Dis Model Mech. 9:1125–1137. 2016.PubMed/NCBI View Article : Google Scholar

6 

Fan Q, Cavus O, Xiong L and Xia Y: Spinal cord injury: How could acupuncture help? J Acupunct Meridian Stud. 11:124–132. 2018.PubMed/NCBI View Article : Google Scholar

7 

Varma AK, Das A, Wallace G IV, Barry J, Vertegel AA, Ray SK and Banik NL: Spinal cord injury: A review of current therapy, future treatments, and basic science frontiers. Neurochem Res. 38:895–905. 2013.PubMed/NCBI View Article : Google Scholar

8 

Cristante AF, Filho TE, Marcon RM, Letaif OB and Rocha ID: Therapeutic approaches for spinal cord injury. Clinics (Sao Paulo). 67:1219–1224. 2012.PubMed/NCBI View Article : Google Scholar

9 

Ravikumar K and Sridhar B: Lenalidomide, an antineoplastic drug, and its hemihydrate. Acta Crystallogr C. 65:o502–o505. 2009.PubMed/NCBI View Article : Google Scholar

10 

Weisel K and Kanz L: Lenalidomide. Recent Results Cancer Res. 201:347–357. 2014.PubMed/NCBI View Article : Google Scholar

11 

Valera E, Mante M, Anderson S, Rockenstein E and Masliah E: Lenalidomide reduces microglial activation and behavioral deficits in a transgenic model of Parkinson's disease. J Neuroinflammation. 12(93)2015.PubMed/NCBI View Article : Google Scholar

12 

Mosley RL and Gendelman HE: Control of neuroinflammation as a therapeutic strategy for amyotrophic lateral sclerosis and other neurodegenerative disorders. Exp Neurol. 222:1–5. 2010.PubMed/NCBI View Article : Google Scholar

13 

Yamamoto K, Kitawaki T, Sugimoto N, Fujita H, Kawase Y, Takaori-Kondo A and Kadowaki N: Anti-inflammatory modulation of human myeloid-derived dendritic cell subsets by lenalidomide. Immunol Lett. 211:41–48. 2019.PubMed/NCBI View Article : Google Scholar

14 

Ding W, Zeng T, Tao W, Ge W, Deng V, Lei H, Xiao Y and Liao F: Effect of lenalidomide on the human gastric cancer cell line SGC7901/vincristine notch signaling. J Cancer Res Ther. 14:S237–S242. 2018.PubMed/NCBI View Article : Google Scholar

15 

Guo J, Fei C, Zhao Y, Zhao S, Zheng Q, Su J, Wu D, Li X and Chang C: Lenalidomide restores the osteogenic differentiation of bone marrow mesenchymal stem cells from multiple myeloma patients via deactivating notch signaling pathway. Oncotarget. 8:55405–55421. 2017.PubMed/NCBI View Article : Google Scholar

16 

Yi G, Liu L, Lv C, Wei Y and Yan T: Ginsenoside Rg1 defenses PC-12cells against hydrogen peroxide-caused damage via up-regulation of miR-216a-5p. Life Sci. 236(116948)2019.PubMed/NCBI View Article : Google Scholar

17 

Wen F, Wong HK, Tay CY, Yu H, Li H, Yu T, Tijore A, Boey FY, Venkatraman SS and Tan LP: Induction of myogenic differentiation of human mesenchymal stem cells cultured on notch agonist (Jagged-1) modified biodegradable scaffold surface. ACS Appl Mater Interfaces. 6:1652–1661. 2014.PubMed/NCBI View Article : Google Scholar

18 

Yue JK, Chan AK, Winkler EA, Upadhyayula PS, Readdy WJ and Dhall SS: A review and update on the guidelines for the acute management of cervical spinal cord injury-part II. J Neurosurg Sci. 60:367–384. 2016.PubMed/NCBI

19 

Siriphorn A, Chompoopong S and Floyd CL: 17β-estradiol protects Schwann cells against H2O2-induced cytotoxicity and increases transplanted Schwann cell survival in a cervical hemicontusion spinal cord injury model. J Neurochem. 115:864–872. 2010.PubMed/NCBI View Article : Google Scholar

20 

Li X, Zhan J, Hou Y, Hou Y, Chen S, Luo D, Luan J, Wang L and Lin D: Coenzyme Q10 regulation of apoptosis and oxidative stress in H2O2 induced BMSC death by modulating the Nrf-2/NQO-1 signaling pathway and its application in a model of spinal cord injury. Oxid Med Cell Longev. 2019(6493081)2019.PubMed/NCBI View Article : Google Scholar

21 

Talati C, Sallman D and List A: Lenalidomide: Myelodysplastic syndromes with del(5q) and beyond. Semin Hematol. 54:159–166. 2017.PubMed/NCBI View Article : Google Scholar

22 

Flowers CR, Leonard JP and Fowler NH: Lenalidomide in follicular lymphoma. Blood. 135:2133–2136. 2020.PubMed/NCBI View Article : Google Scholar

23 

Jia Z, Zhu H, Li J, Wang X, Misra H and Li Y: Oxidative stress in spinal cord injury and antioxidant-based intervention. Spinal Cord. 50:264–274. 2012.PubMed/NCBI View Article : Google Scholar

24 

Hall ED: Antioxidant therapies for acute spinal cord injury. Neurotherapeutics. 8:152–167. 2011.PubMed/NCBI View Article : Google Scholar

25 

Zhu X, Jiang S, Hu N, Luo F, Dong H, Kang YM, Jones KR, Zou Y, Xiong L and Ren J: Tumour necrosis factor-alpha inhibition with lenalidomide alleviates tissue oxidative injury and apoptosis in ob/ob obese mice. Clin Exp Pharmacol Physiol. 41:489–501. 2014.PubMed/NCBI View Article : Google Scholar

26 

Li L, Hua Y, Dong M, Li Q, Smith DT, Yuan M, Jones KR and Ren J: Short-term lenalidomide (Revlimid) administration ameliorates cardiomyocyte contractile dysfunction in ob/ob obese mice. Obesity (Silver Spring). 20:2174–2185. 2012.PubMed/NCBI View Article : Google Scholar

27 

Eitan E, Hutchison ER, Greig NH, Tweedie D, Celik H, Ghosh S, Fishbein KW, Spencer RG, Sasaki CY, Ghosh P, et al: Combination therapy with lenalidomide and nanoceria ameliorates CNS autoimmunity. Exp Neurol. 273:151–160. 2015.PubMed/NCBI View Article : Google Scholar

28 

Zhang N, Yin Y, Xu SJ, Wu YP and Chen WS: Inflammation & apoptosis in spinal cord injury. Indian J Med Res. 135:287–296. 2012.PubMed/NCBI

29 

Keane RW, Davis AR and Dietrich WD: Inflammatory and apoptotic signaling after spinal cord injury. J Neurotrauma. 23:335–344. 2006.PubMed/NCBI View Article : Google Scholar

30 

Qu X, Mei J, Yu Z, Zhai Z, Qiao H and Dai K: Lenalidomide regulates osteocytes fate and related osteoclastogenesis via IL-1β/NF-kappaB/RANKL signaling. Biochem Biophys Res Commun. 501:547–555. 2018.PubMed/NCBI View Article : Google Scholar

31 

Cai Z, Zhao B, Deng Y, Shangguan S, Zhou F, Zhou W, Li X, Li Y and Chen G: Notch signaling in cerebrovascular diseases (Review). Mol Med Rep. 14:2883–2898. 2016.PubMed/NCBI View Article : Google Scholar

32 

Wei H, Huang L, Wei F, Li G, Huang B, Li J and Cao C: 2020. Up-regulation of miR-139-5p protects diabetic mice from liver tissue damage and oxidative stress through inhibiting notch signaling pathway. Acta Biochim Biophys Sin (Shanghai). 52:390–400. 2016.PubMed/NCBI View Article : Google Scholar

33 

Zhang S, Botchway BOA, Zhang Y and Liu X: Resveratrol can inhibit notch signaling pathway to improve spinal cord injury. Ann Anat. 223:100–107. 2019.PubMed/NCBI View Article : Google Scholar

34 

Zhang Y, Gao T, Li X, Wen CC, Yan XT, Peng C and Xiao Y: Circ_0005075 targeting miR-151a-3p promotes neuropathic pain in CCI rats via inducing NOTCH2 expression. Gene. 767(145079)2021.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Lv Z, Yin S, Cheng Z and Wang K: Lenalidomide improves H<sub>2</sub>O<sub>2</sub>‑induced PC12 cell injury by blocking the Notch signaling pathway. Exp Ther Med 23: 421, 2022.
APA
Lv, Z., Yin, S., Cheng, Z., & Wang, K. (2022). Lenalidomide improves H<sub>2</sub>O<sub>2</sub>‑induced PC12 cell injury by blocking the Notch signaling pathway. Experimental and Therapeutic Medicine, 23, 421. https://doi.org/10.3892/etm.2022.11348
MLA
Lv, Z., Yin, S., Cheng, Z., Wang, K."Lenalidomide improves H<sub>2</sub>O<sub>2</sub>‑induced PC12 cell injury by blocking the Notch signaling pathway". Experimental and Therapeutic Medicine 23.6 (2022): 421.
Chicago
Lv, Z., Yin, S., Cheng, Z., Wang, K."Lenalidomide improves H<sub>2</sub>O<sub>2</sub>‑induced PC12 cell injury by blocking the Notch signaling pathway". Experimental and Therapeutic Medicine 23, no. 6 (2022): 421. https://doi.org/10.3892/etm.2022.11348
Copy and paste a formatted citation
x
Spandidos Publications style
Lv Z, Yin S, Cheng Z and Wang K: Lenalidomide improves H<sub>2</sub>O<sub>2</sub>‑induced PC12 cell injury by blocking the Notch signaling pathway. Exp Ther Med 23: 421, 2022.
APA
Lv, Z., Yin, S., Cheng, Z., & Wang, K. (2022). Lenalidomide improves H<sub>2</sub>O<sub>2</sub>‑induced PC12 cell injury by blocking the Notch signaling pathway. Experimental and Therapeutic Medicine, 23, 421. https://doi.org/10.3892/etm.2022.11348
MLA
Lv, Z., Yin, S., Cheng, Z., Wang, K."Lenalidomide improves H<sub>2</sub>O<sub>2</sub>‑induced PC12 cell injury by blocking the Notch signaling pathway". Experimental and Therapeutic Medicine 23.6 (2022): 421.
Chicago
Lv, Z., Yin, S., Cheng, Z., Wang, K."Lenalidomide improves H<sub>2</sub>O<sub>2</sub>‑induced PC12 cell injury by blocking the Notch signaling pathway". Experimental and Therapeutic Medicine 23, no. 6 (2022): 421. https://doi.org/10.3892/etm.2022.11348
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