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Germacrone induces caspase‑3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis

  • Authors:
    • Xinfeng Sun
    • Xin Zhong
    • Wenfeng Ma
    • Wenxing Feng
    • Qi Huang
    • Mengqing Ma
    • Minling Lv
    • Rui Hu
    • Zhiyi Han
    • Jing Li
    • Xiaozhou Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Liver Disease, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, Guangdong 518033, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 456
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    Published online on: May 20, 2022
       https://doi.org/10.3892/etm.2022.11383
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Abstract

Liver cancer is a highly lethal malignancy. Despite considerable efforts made in recent years, the prognosis of patients with liver cancer remains poor. Curcuma zedoaria (known as Ezhu in Chinese) is widely prescribed in traditional Chinese medicine. Germacrone (GM) is a sesquiterpene constituent derived from the essential oil of Ezhu, and exerts anti‑carcinogenic effects by inducing apoptosis in various cancer cells. The present study investigated the potential mechanism of GM in HepG2 cells. Cell Counting Kit‑8, colony‑formation and lactate dehydrogenase‑release assays, as well as cell death assays using flow cytometry, were performed to evaluate HepG2 cell proliferation following GM treatment. HepG2 cells were transfected with caspase‑3 small interfering RNA and then treated with GM. Caspase‑3 expression levels were determined by reverse transcription‑quantitative PCR and western blotting. The present study showed that GM inhibited the growth of HepG2 cells and induced the proteolytic cleavage of caspase 3, with concomitant cleavage of gasdermin E (GSDME), by markedly increasing the production of reactive oxygen species (ROS). This led to caspase 3‑dependent cleavage of GSDME, thereby promoting pyroptosis in HepG2 cells. However, these changes were rescued by ROS scavengers, such as N‑acetylcysteine. Furthermore, GM inhibited tumor growth by promoting the cleavage of caspase 3 and GSDME in HepG2 cell xenograft models. These results indicated that GM induced GSDME‑dependent pyroptosis through caspase 3 activation, at least in part, by damaging the mitochondria and enhancing ROS production, thereby supporting the possible development of GM as a candidate for the prevention and treatment of liver cancer.
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1 

Clark T, Maximin S, Meier J, Pokharel S and Bhargava P: Hepatocellular carcinoma: Review of epidemiology, screening, imaging diagnosis, response assessment, and treatment. Curr Probl Diagn Radiol. 44:479–486. 2015.PubMed/NCBI View Article : Google Scholar

2 

Hartke J, Johnson M and Ghabril M: The diagnosis and treatment of hepatocellular carcinoma. Semin Diagn Pathol. 34:153–159. 2017.PubMed/NCBI View Article : Google Scholar

3 

Sia D, Villanueva A, Friedman SL and Llovet JM: Liver cancer cell of origin, molecular class, and effects on patient prognosis. Gastroenterology. 152:745–761. 2017.PubMed/NCBI View Article : Google Scholar

4 

Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A and Bray F: Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 71:209–249. 2021.PubMed/NCBI View Article : Google Scholar

5 

Oravecz M and Mészáros J: Traditional Chinese medicine: Theoretical background and its use in China. Orv Hetil. 153:723–731. 2012.PubMed/NCBI View Article : Google Scholar : (In Hungarian).

6 

Chan HHL and Ng T: Traditional Chinese medicine (TCM) and allergic diseases. Curr Allergy Asthma Rep. 20(67)2020.PubMed/NCBI View Article : Google Scholar

7 

Zhou P: Traditional Chinese medicine. Comb Chem High Throughput Screen. 13(836)2010.PubMed/NCBI View Article : Google Scholar

8 

Momenkiaei F and Raofie F: Preparation of Curcuma longa L. extract nanoparticles using supercritical solution expansion. J Pharm Sci. 108:1581–1589. 2019.PubMed/NCBI View Article : Google Scholar

9 

Dosoky NS and Setzer WN: Chemical composition and biological activities of essential oils of Curcuma species. Nutrients. 10(1196)2018.PubMed/NCBI View Article : Google Scholar

10 

Sun J, Chen F, Braun C, Zhou YQ, Rittner H, Tian YK, Cai XY and Ye DW: Role of curcumin in the management of pathological pain. Phytomedicine. 48:129–140. 2018.PubMed/NCBI View Article : Google Scholar

11 

Ayati Z, Ramezani M, Amiri MS, Moghadam AT, Rahimi H, Abdollahzade A, Sahebkar A and Emami SA: Ethnobotany, phytochemistry and traditional uses of Curcuma spp. and pharmacological profile of two important species (C. longa and C. zedoaria): A review. Curr Pharm Des. 25:871–935. 2019.PubMed/NCBI View Article : Google Scholar

12 

Li Y, Wu Y, Li Y and Guo F: Review of the traditional uses, phytochemistry, and pharmacology of Curcuma wenyujin Y. H. Chen et C. Ling. J Ethnopharmacol. 269(113689)2021.PubMed/NCBI View Article : Google Scholar

13 

Dosoky NS, Satyal P and Setzer WN: Variations in the volatile compositions of Curcuma species. Foods. 8(53)2019.PubMed/NCBI View Article : Google Scholar

14 

Goel A and Aggarwal BB: Curcumin, the golden spice from Indian saffron, is a chemosensitizer and radiosensitizer for tumors and chemoprotector and radioprotector for normal organs. Nutr Cancer. 62:919–930. 2010.PubMed/NCBI View Article : Google Scholar

15 

Giordano A and Tommonaro G: Curcumin and cancer. Nutrients. 11(2376)2019.PubMed/NCBI View Article : Google Scholar

16 

Burapan S, Kim M, Paisooksantivatana Y, Eser BE and Han J: Thai Curcuma species: Antioxidant and bioactive compounds. Foods. 9(1219)2020.PubMed/NCBI View Article : Google Scholar

17 

Srivilai J, Waranuch N, Tangsumranjit A, Khorana N and Ingkaninan K: Germacrone and sesquiterpene-enriched extracts from Curcuma aeruginosa Roxb. Increase skin penetration of minoxidil, a hair growth promoter. Drug Deliv Transl Res. 8:140–149. 2018.PubMed/NCBI View Article : Google Scholar

18 

Liu Y, Wang W, Fang B, Ma F, Zheng Q, Deng P, Zhao S, Chen M, Yang G and He G: Anti-tumor effect of germacrone on human hepatoma cell lines through inducing G2/M cell cycle arrest and promoting apoptosis. Eur J Pharmacol. 698:95–102. 2013.PubMed/NCBI View Article : Google Scholar

19 

Riaz A, Rasul A, Kanwal N, Hussain G, Shah MA, Sarfraz I, Ishfaq R, Batool R, Rukhsar F and Adem Ş: Germacrone: A potent secondary metabolite with therapeutic potential in metabolic diseases, cancer and viral infections. Curr Drug Metab. 21:1079–1090. 2020.PubMed/NCBI View Article : Google Scholar

20 

Zhang R, Hao J, Guo K, Liu W, Yao F, Wu Q, Liu C, Wang Q and Yang X: Germacrone inhibits cell proliferation and induces apoptosis in human esophageal squamous cell carcinoma cells. Biomed Res Int. 2020(7643248)2020.PubMed/NCBI View Article : Google Scholar

21 

Kovacs SB and Miao EA: Gasdermins: Effectors of pyroptosis. Trends Cell Biol. 27:673–684. 2017.PubMed/NCBI View Article : Google Scholar

22 

Shi J, Gao W and Shao F: Pyroptosis: Gasdermin-mediated programmed necrotic cell death. Trends Biochem Sci. 42:245–254. 2017.PubMed/NCBI View Article : Google Scholar

23 

Jia C, Chen H, Zhang J, Zhou K, Zhuge Y, Niu C, Qiu J, Rong X, Shi Z, Xiao J, et al: Role of pyroptosis in cardiovascular diseases. Int Immunopharmacol. 67:311–318. 2019.PubMed/NCBI View Article : Google Scholar

24 

Shi J, Zhao Y, Wang K, Shi X, Wang Y, Huang H, Zhuang Y, Cai T, Wang F and Shao F: Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature. 526:660–665. 2015.PubMed/NCBI View Article : Google Scholar

25 

Sun L, Ma W, Gao W, Xing Y, Chen L, Xia Z, Zhang Z and Dai Z: Propofol directly induces caspase-1-dependent macrophage pyroptosis through the NLRP3-ASC inflammasome. Cell Death Dis. 10(542)2019.PubMed/NCBI View Article : Google Scholar

26 

Wang Y, Gao W, Shi X, Ding J, Liu W, He H, Wang K and Shao F: Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a gasdermin. Nature. 547:99–103. 2017.PubMed/NCBI View Article : Google Scholar

27 

Jiang M, Qi L, Li L and Li Y: The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer. Cell Death Discov. 6(112)2020.PubMed/NCBI View Article : Google Scholar

28 

Liang WF, Gong YX, Li HF, Sun FL, Li WL, Chen DQ, Xie DP, Ren CX, Guo XY, Wang ZY, et al: Curcumin activates ROS signaling to promote pyroptosis in hepatocellular carcinoma HepG2 cells. In Vivo. 35:249–257. 2021.PubMed/NCBI View Article : Google Scholar

29 

Zhang X, Zhang P, An L, Sun N, Peng L, Tang W, Ma D and Chen J: Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. Acta Pharm Sin B. 10:1397–1413. 2020.PubMed/NCBI View Article : Google Scholar

30 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

31 

Zhan ZY, Wu M, Shang Y, Jiang M, Liu J, Qiao CY, Ye H, Lin YC, Piao MH, Sun RH, et al: Taxifolin ameliorate high-fat-diet feeding plus acute ethanol binge-induced steatohepatitis through inhibiting inflammatory caspase-1-dependent pyroptosis. Food Funct. 12:362–372. 2021.PubMed/NCBI View Article : Google Scholar

32 

Yu J, Li S, Qi J, Chen Z, Wu Y, Guo J, Wang K, Sun X and Zheng J: Cleavage of GSDME by caspase-3 determines lobaplatin-induced pyroptosis in colon cancer cells. Cell Death Dis. 10(193)2019.PubMed/NCBI View Article : Google Scholar

33 

Kleih M, Böpple K, Dong M, Gaißler A, Heine S, Olayioye MA, Aulitzky WE and Essmann F: Direct impact of cisplatin on mitochondria induces ROS production that dictates cell fate of ovarian cancer cells. Cell Death Dis. 10(851)2019.PubMed/NCBI View Article : Google Scholar

34 

Li Q, Liang X, Yang Y, Zeng X, Zhong X and Huang C: Panax notoginseng saponins ameliorate cisplatin-induced mitochondrial injury via the HIF-1α/mitochondria/ROS pathway. FEBS Open Bio. 10:118–126. 2020.PubMed/NCBI View Article : Google Scholar

35 

Mazat JP, Devin A and Ransac S: Modelling mitochondrial ROS production by the respiratory chain. Cell Mol Life Sci. 77:455–465. 2020.PubMed/NCBI View Article : Google Scholar

36 

Li Q, Shi N, Cai C, Zhang M, He J, Tan Y and Fu W: The role of mitochondria in pyroptosis. Front Cell Dev Biol. 8(630771)2021.PubMed/NCBI View Article : Google Scholar

37 

Wang K, Sun Q, Zhong X, Zeng M, Zeng H, Shi X, Li Z, Wang Y, Zhao Q, Shao F and Ding J: Structural mechanism for GSDMD targeting by autoprocessed caspases in pyroptosis. Cell. 180:941–955.e20. 2020.PubMed/NCBI View Article : Google Scholar

38 

Wu LS, Liu Y, Wang XW, Xu B, Lin YL, Song Y, Dong Y, Liu JL, Wang XJ, Liu S, et al: LPS enhances the chemosensitivity of oxaliplatin in HT29 cells via GSDMD-mediated pyroptosis. Cancer Manag Res. 12:10397–10409. 2020.PubMed/NCBI View Article : Google Scholar

39 

An H, Heo JS, Kim P, Lian Z, Lee S, Park J, Hong E, Pang K, Park Y, Ooshima A, et al: Tetraarsenic hexoxide enhances generation of mitochondrial ROS to promote pyroptosis by inducing the activation of caspase-3/GSDME in triple-negative breast cancer cells. Cell Death Dis. 12(159)2021.PubMed/NCBI View Article : Google Scholar

40 

Zhao Y, Cai J, Shi K, Li H, Du J, Hu D, Liu Z and Wang W: Germacrone induces lung cancer cell apoptosis and cell cycle arrest via the Akt/MDM2/p53 signaling pathway. Mol Med Rep. 23(452)2021.PubMed/NCBI View Article : Google Scholar

41 

Zhong Z, Chen X, Tan W, Xu Z, Zhou K, Wu T, Cui L and Wang Y: Germacrone inhibits the proliferation of breast cancer cell lines by inducing cell cycle arrest and promoting apoptosis. Eur J Pharmacol. 667:50–55. 2011.PubMed/NCBI View Article : Google Scholar

42 

Fang X, Tan T, Gao B, Zhao Y, Liu T and Xia Q: Germacrone regulates HBXIP-mediated cell cycle, apoptosis and promotes the formation of autophagosomes to inhibit the proliferation of gastric cancer cells. Front Oncol. 10(537322)2020.PubMed/NCBI View Article : Google Scholar

43 

Liu YY, Zheng Q, Fang B, Wang W, Ma FY, Roshan S, Banafa A, Chen MJ, Chang JL, Deng XM, et al: Germacrone induces apoptosis in human hepatoma HepG2 cells through inhibition of the JAK2/STAT3 signalling pathway. J Huazhong Univ Sci Technolog Med Sci. 33:339–345. 2013.PubMed/NCBI View Article : Google Scholar

44 

Cui Y, Peng Y, Zhang Q, Xia S, Ruan B, Xu Q, Yu X, Zhou T, Liu H, Zeng D, et al: Disruption of EARLY LESION LEAF 1, encoding a cytochrome P450 monooxygenase, induces ROS accumulation and cell death in rice. Plant J. 105:942–956. 2021.PubMed/NCBI View Article : Google Scholar

45 

He L, He T, Farrar S, Ji L, Liu T and Ma X: Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cell Physiol Biochem. 44:532–553. 2017.PubMed/NCBI View Article : Google Scholar

46 

Lu Y and Cederbaum AI: Cytochrome P450s and alcoholic liver disease. Curr Pharm Des. 24:1502–1517. 2018.PubMed/NCBI View Article : Google Scholar

47 

Chien Y, Rosal K and Chung BC: Function of CYP11A1 in the mitochondria. Mol Cell Endocrinol. 441:55–61. 2017.PubMed/NCBI View Article : Google Scholar

48 

Peter Guengerich F and Avadhani NG: Roles of cytochrome P450 in metabolism of ethanol and carcinogens. Adv Exp Med Biol. 1032:15–35. 2018.PubMed/NCBI View Article : Google Scholar

49 

Guengerich FP: Cytochrome P450 2E1 and its roles in disease. Chem Biol Interact. 322(109056)2020.PubMed/NCBI View Article : Google Scholar

50 

Zhou B, Zhang JY, Liu XS, Chen HZ, Ai YL, Cheng K, Sun RY, Zhou D, Han J and Wu Q: Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis. Cell Res. 28:1171–1185. 2018.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Sun X, Zhong X, Ma W, Feng W, Huang Q, Ma M, Lv M, Hu R, Han Z, Li J, Li J, et al: Germacrone induces caspase‑3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis. Exp Ther Med 24: 456, 2022.
APA
Sun, X., Zhong, X., Ma, W., Feng, W., Huang, Q., Ma, M. ... Zhou, X. (2022). Germacrone induces caspase‑3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis. Experimental and Therapeutic Medicine, 24, 456. https://doi.org/10.3892/etm.2022.11383
MLA
Sun, X., Zhong, X., Ma, W., Feng, W., Huang, Q., Ma, M., Lv, M., Hu, R., Han, Z., Li, J., Zhou, X."Germacrone induces caspase‑3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis". Experimental and Therapeutic Medicine 24.1 (2022): 456.
Chicago
Sun, X., Zhong, X., Ma, W., Feng, W., Huang, Q., Ma, M., Lv, M., Hu, R., Han, Z., Li, J., Zhou, X."Germacrone induces caspase‑3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis". Experimental and Therapeutic Medicine 24, no. 1 (2022): 456. https://doi.org/10.3892/etm.2022.11383
Copy and paste a formatted citation
x
Spandidos Publications style
Sun X, Zhong X, Ma W, Feng W, Huang Q, Ma M, Lv M, Hu R, Han Z, Li J, Li J, et al: Germacrone induces caspase‑3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis. Exp Ther Med 24: 456, 2022.
APA
Sun, X., Zhong, X., Ma, W., Feng, W., Huang, Q., Ma, M. ... Zhou, X. (2022). Germacrone induces caspase‑3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis. Experimental and Therapeutic Medicine, 24, 456. https://doi.org/10.3892/etm.2022.11383
MLA
Sun, X., Zhong, X., Ma, W., Feng, W., Huang, Q., Ma, M., Lv, M., Hu, R., Han, Z., Li, J., Zhou, X."Germacrone induces caspase‑3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis". Experimental and Therapeutic Medicine 24.1 (2022): 456.
Chicago
Sun, X., Zhong, X., Ma, W., Feng, W., Huang, Q., Ma, M., Lv, M., Hu, R., Han, Z., Li, J., Zhou, X."Germacrone induces caspase‑3/GSDME activation and enhances ROS production, causing HepG2 pyroptosis". Experimental and Therapeutic Medicine 24, no. 1 (2022): 456. https://doi.org/10.3892/etm.2022.11383
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