miR‑30e‑5p attenuates neuronal deficit and inflammation of rats with intracerebral hemorrhage by regulating TLR4

Song,Haipeng; Xu,Na; Jin,Shan

doi:10.3892/etm.2022.11419

miR‑30e‑5p attenuates neuronal deficit and inflammation of rats with intracerebral hemorrhage by regulating TLR4

Authors:
- Haipeng Song
- Na Xu
- Shan Jin
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Affiliations: Department of Neurosurgery, Jinan First People's Hospital, Ji'nan, Shandong 250012, P.R. China, Department of Emergency, Jinan First People's Hospital, Ji'nan, Shandong 250012, P.R. China, Department of Neurology, The 960th Hospital of The Chinese People's Liberation Army, Ji'nan, Shandong 250031, P.R. China
Published online on: June 7, 2022 https://doi.org/10.3892/etm.2022.11419
Article Number: 492
Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

microRNAs (miRNAs or miRs) have been reported to regulate the pathology of intracerebral hemorrhage (ICH). Therefore, the present study aimed to investigate the function of miR‑30e‑5p in rats with ICH with specific focus on Toll‑like receptor (TLR)4. In the present study, collagenase type IV was used for the establishment of the ICH model in rats, prior to which the rats were injected with miR‑30e‑5p mimic or miR‑30e‑5p mimic + pcDNA3.1‑TLR4 plasmid. The expression levels of miR‑30e‑5p and TLR4 were then measured using reverse transcription‑quantitative PCR and western blotting. The potential interaction between miR‑30e‑5p and TLR4 was tested using the MicroRNA Target Prediction Database and dual‑luciferase reporter and RNA immunoprecipitation assay. In addition, the concentration of TNF‑α, IL‑6 and IL‑1β was measured using ELISA. The protein expression levels of TLR4/myeloid differentiation factor 88 (MyD88)/TIR‑domain‑containing adapter‑inducing interferon‑β (TRIF) signaling‑associated molecules were measured by western blotting. Following induction of ICH, miR‑30e‑5p expression was downregulated, while TLR4 expression was upregulated. By contrast, injection with miR‑30e‑5p mimic rescued neuronal function while suppressing neuronal inflammation in rats following ICH; these effects were reversed by co‑overexpression of TLR4. Furthermore, overexpression of miR‑30e‑5p inactivated TLR4/MyD88/TRIF signaling in rats with ICH; this was also reversed by overexpression of TLR4. Taken together, these results suggested that overexpression of miR‑30e‑5p exerted a protective role against neuronal deficit and inflammation caused by ICH in rats by targeting TLR4 and inactivating TLR4/MyD88/TRIF signaling.

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