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IL‑10/IL‑10 receptor 1 pathway promotes the viability and collagen synthesis of pulmonary fibroblasts originated from interstitial pneumonia tissues

  • Authors:
    • Hong Ye
    • Jiongwei Pan
    • Xiaoping Cai
    • Zhangyong Yin
    • Lu Li
    • Enhui Gong
    • Cunlai Xu
    • Hao Zheng
    • Zhuo Cao
    • Enguo Chen
    • Junfeng Qian
  • View Affiliations / Copyright

    Affiliations: Respiratory Department, The Sixth Affiliated Hospital of Wenzhou Medical University/Lishui People's Hospital, Lishui, Zheijang 323000, P.R. China, Department of Respiratory and Critical Care Medicine, Sir Run Run Shaw Hospital, Affiliated to Zhejiang University School of Medicine, Hangzhou, Zheijang 310016, P.R. China
    Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 518
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    Published online on: June 15, 2022
       https://doi.org/10.3892/etm.2022.11445
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Abstract

Interstitial pneumonia is a pulmonary interstitial inflammatory and fibrosis disease with a variety of causes that causes respiratory disorders and threatens the lives of patients. The present study aimed to investigate the expression of interleukin (IL)‑10 in peripheral blood of patients with interstitial pneumonia and its biological functions in pulmonary fibroblasts. A total of 42 patients with idiopathic pulmonary fibrosis (IPF) and 20 healthy subjects were included. ELISA was used to determine IL‑10 concentration in serum from the patients and healthy subjects. Primary fibroblasts were isolated from lung tissue successfully and determined by morphology. The CCK‑8 assay was performed to determine the effect of IL‑10 expression on cell viability. Western blotting was used to determine COL1a1, COL1a2 and IL‑10R1 protein expression. Flow cytometry was used for cell cycle analysis and to determine the number of IL‑10+ cells. Expression of IL‑10 in serum from IPF patients was higher compared to that from healthy subjects. IL‑10 promoted the viability and collagen synthesis and secretion of MRC‑5 cells and primary pulmonary fibroblasts. IL‑10 and IL‑10 receptor (R) 1 served regulatory roles in the viability and collagen synthesis of MRC‑5 cells. The ratio of peripheral mononuclear lymphocytes with positive expression of IL‑10 was elevated in peripheral blood from patients with IPF. The present study demonstrated that IL‑10 expression in peripheral blood of patients with IPF is increased significantly compared with healthy subjects. Activation of the IL‑10/IL‑10R1 signaling pathway promoted the viability and collagen synthesis and secretion of pulmonary fibroblasts, leading to pulmonary fibrosis. The present study provided experimental basis for further understanding the development mechanism of pulmonary fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Ye H, Pan J, Cai X, Yin Z, Li L, Gong E, Xu C, Zheng H, Cao Z, Chen E, Chen E, et al: IL‑10/IL‑10 receptor 1 pathway promotes the viability and collagen synthesis of pulmonary fibroblasts originated from interstitial pneumonia tissues. Exp Ther Med 24: 518, 2022.
APA
Ye, H., Pan, J., Cai, X., Yin, Z., Li, L., Gong, E. ... Qian, J. (2022). IL‑10/IL‑10 receptor 1 pathway promotes the viability and collagen synthesis of pulmonary fibroblasts originated from interstitial pneumonia tissues. Experimental and Therapeutic Medicine, 24, 518. https://doi.org/10.3892/etm.2022.11445
MLA
Ye, H., Pan, J., Cai, X., Yin, Z., Li, L., Gong, E., Xu, C., Zheng, H., Cao, Z., Chen, E., Qian, J."IL‑10/IL‑10 receptor 1 pathway promotes the viability and collagen synthesis of pulmonary fibroblasts originated from interstitial pneumonia tissues". Experimental and Therapeutic Medicine 24.2 (2022): 518.
Chicago
Ye, H., Pan, J., Cai, X., Yin, Z., Li, L., Gong, E., Xu, C., Zheng, H., Cao, Z., Chen, E., Qian, J."IL‑10/IL‑10 receptor 1 pathway promotes the viability and collagen synthesis of pulmonary fibroblasts originated from interstitial pneumonia tissues". Experimental and Therapeutic Medicine 24, no. 2 (2022): 518. https://doi.org/10.3892/etm.2022.11445
Copy and paste a formatted citation
x
Spandidos Publications style
Ye H, Pan J, Cai X, Yin Z, Li L, Gong E, Xu C, Zheng H, Cao Z, Chen E, Chen E, et al: IL‑10/IL‑10 receptor 1 pathway promotes the viability and collagen synthesis of pulmonary fibroblasts originated from interstitial pneumonia tissues. Exp Ther Med 24: 518, 2022.
APA
Ye, H., Pan, J., Cai, X., Yin, Z., Li, L., Gong, E. ... Qian, J. (2022). IL‑10/IL‑10 receptor 1 pathway promotes the viability and collagen synthesis of pulmonary fibroblasts originated from interstitial pneumonia tissues. Experimental and Therapeutic Medicine, 24, 518. https://doi.org/10.3892/etm.2022.11445
MLA
Ye, H., Pan, J., Cai, X., Yin, Z., Li, L., Gong, E., Xu, C., Zheng, H., Cao, Z., Chen, E., Qian, J."IL‑10/IL‑10 receptor 1 pathway promotes the viability and collagen synthesis of pulmonary fibroblasts originated from interstitial pneumonia tissues". Experimental and Therapeutic Medicine 24.2 (2022): 518.
Chicago
Ye, H., Pan, J., Cai, X., Yin, Z., Li, L., Gong, E., Xu, C., Zheng, H., Cao, Z., Chen, E., Qian, J."IL‑10/IL‑10 receptor 1 pathway promotes the viability and collagen synthesis of pulmonary fibroblasts originated from interstitial pneumonia tissues". Experimental and Therapeutic Medicine 24, no. 2 (2022): 518. https://doi.org/10.3892/etm.2022.11445
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