Open Access

Wnt3a knockdown promotes collagen type II expression in rat chondrocytes

  • Authors:
    • Shiping Shi
    • Zhentao Man
    • Shui Sun
  • View Affiliations

  • Published online on: June 17, 2022     https://doi.org/10.3892/etm.2022.11453
  • Article Number: 526
  • Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteoarthritis (OA) is a chronic condition caused by cartilage degradation, and there are currently no effective methods for preventing the progression of this disease; gene therapy is a relatively novel method for treating arthritis. Decreased collagen type II (Col2) expression within the cartilage matrix is an important factor for the development of OA, and Wnt3a serves a significant role in cartilage homeostasis. The present study assessed whether Wnt3a knockdown promoted Col2 expression in chondrocytes. Lentivirus‑introduced small interfering RNA was used to knock down the expression of Wnt3a in primary rat chondrocytes, and then IL‑1β treatment was used to establish an OA chondrocyte model. The expression of target genes (Wnt3a, Col2, MMP‑13 and β‑catenin) was analyzed using reverse transcription‑quantitative PCR, western blotting and immunocytochemistry. There was significantly less MMP‑13 and β‑catenin expression in the Wnt3a knockdown cells compared with the other controls. Col2 expression was significantly higher in the Wnt3a‑knockdown cells compared with the control cells, indicating that knockdown of Wnt3a may promote Col2 expression. Consequently, Wnt3a was indicated to be an important factor in cartilage homeostasis, and Wnt3a knockdown may serve as a novel method for OA therapy.
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August-2022
Volume 24 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Shi S, Man Z and Sun S: Wnt3a knockdown promotes collagen type II expression in rat chondrocytes. Exp Ther Med 24: 526, 2022
APA
Shi, S., Man, Z., & Sun, S. (2022). Wnt3a knockdown promotes collagen type II expression in rat chondrocytes. Experimental and Therapeutic Medicine, 24, 526. https://doi.org/10.3892/etm.2022.11453
MLA
Shi, S., Man, Z., Sun, S."Wnt3a knockdown promotes collagen type II expression in rat chondrocytes". Experimental and Therapeutic Medicine 24.2 (2022): 526.
Chicago
Shi, S., Man, Z., Sun, S."Wnt3a knockdown promotes collagen type II expression in rat chondrocytes". Experimental and Therapeutic Medicine 24, no. 2 (2022): 526. https://doi.org/10.3892/etm.2022.11453