Genetic analysis of recurrent parthenogenesis: A case report and literature review

Jiang,Yan; Song,Ge; Yuan,Jing-Chuan; Zhang,Xu-Hui; Wu,Xiao-Hua

doi:10.3892/etm.2022.11457

Genetic analysis of recurrent parthenogenesis: A case report and literature review

Authors:
- Yan Jiang
- Ge Song
- Jing-Chuan Yuan
- Xu-Hui Zhang
- Xiao-Hua Wu
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Affiliations: The Center for Reproductive Medicine and Infertility, The Fourth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
Published online on: June 21, 2022 https://doi.org/10.3892/etm.2022.11457
Article Number: 530
Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study reported a case of bilateral salpingectomy for an ectopic pregnancy with recurrent parthenogenesis over two in vitro fertilization (IVF) cycles. The first IVF cycle resulted in short‑time fertilization. Two cleaved embryos were present after removing the cumulus cells. In the second cycle, intracytoplasmic sperm injection (ICSI) was performed directly and two 6‑cell embryos were discovered again prior to the injection. Embryo biopsy, genome amplification, copy number variation (CNV) and single nucleotide polymorphism (SNP) analysis were performed on the two 6‑cell embryos of the second cycle. The results of the CNV analysis indicated a genotype of 39,XX,+1,+1,+1,+1,+6q,+6q,+6q,‑7p(x1),‑10(x1),‑13(x0),‑15(x0),‑17(x1),‑18(x1),‑19(x1),‑20(x1) and the SNP analysis reported that only those chromosomes with one copy had a signal pattern similar to that obtained for an uniparental disomy. Although repeated spontaneous parthenogenesis was observed, the other metaphase II oocytes were fertilized normally after ICSI and the patient became pregnant. A literature review indicated that parthenogenesis may occur in individuals from various populations, and the patients always have a history of either recurrent miscarriages or bilateral tubal obstruction with or without ovarian/fallopian tube surgery. In certain cases, 1 pronucleus (PN) appears and cleaves later and in others, four‑to six‑cell embryos appear directly.

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