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Establishment of a mouse model for bile duct repair and tissue engineering

  • Authors:
    • Xinlan Ge
    • Dabin Xu
    • Ke Pan
    • Guankun Mao
    • Shichun Lu
    • Chonghui Li
  • View Affiliations / Copyright

    Affiliations: Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing 100853, P.R. China, Faculty of Hepato‑Pancreato‑Biliary Surgery, Chinese PLA General Hospital, Beijing 100853, P.R. China
    Copyright: © Ge et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 739
    |
    Published online on: November 2, 2022
       https://doi.org/10.3892/etm.2022.11675
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Abstract

Due to the lack of a suitable model, research on biliary biology is far behind that on other organs. A mouse model of common bile duct (CBD) dilation (BDD) was first established and compared with CBD ligation mice (BDL). Then, in a transplantation experiment, the dilated CBD of recipient BDD mice was injured by making an elliptical incision and repaired by transplanting a bile duct patch from donor BDD mice. Biochemical and histological changes were analyzed and cell proliferation of the bile duct grafts was determined. Slightly dilated and unblocked CBD with a diameter of 2.89±0.76 mm was obtained in BDD mice, while the CBD diameter was 0.51±0.08 mm in the Sham group and 4.71±0.64 mm in the BDL group on day 14 after surgery. The liver damage was very mild in BDD mice compared with BDL mice, proving that the BDD model could be further used for bile duct transplantation. By cross transplanting the bile duct patch from enhanced green fluorescence protein and wild‑type BDD mice, it was found that the CBD injury was well repaired and the cells of the bile duct patch were completely replaced by recipient‑derived cells at 12 week after the repair operation. α Smooth muscle actin, Ki67 and cytokeratin 19 immunofluorescence staining showed that the proliferation of bile duct epithelial cells and abundant active fibroblasts were found within the bile duct patch during the regeneration process. Therefore, a reliable new mouse model of bile duct injury and repair was successfully established and can be used in the study of biliary repair mechanisms and tissue engineering of biliary ducts.
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1 

Pesce A, Palmucci S, La Greca G and Puleo S: Iatrogenic bile duct injury: Impact and management challenges. Clin Exp Gastroenterol. 12:121–128. 2019.PubMed/NCBI View Article : Google Scholar

2 

Booij KAC, Coelen RJ, de Reuver PR, Besselink MG, van Delden OM, Rauws EA, Busch OR, van Gulik TM and Gouma DJ: Long-term follow-up and risk factors for strictures after hepaticojejunostomy for bile duct injury: An analysis of surgical and percutaneous treatment in a tertiary center. Surgery. 163:1121–1127. 2018.PubMed/NCBI View Article : Google Scholar

3 

Booij KAC, de Reuver PR, van Dieren S, van Delden OM, Rauws EA, Busch OR, van Gulik TM and Gouma DJ: Long-term impact of bile duct injury on morbidity, mortality, quality of life, and work related limitations. Ann Surg. 268:143–150. 2018.PubMed/NCBI View Article : Google Scholar

4 

Stilling NM, Fristrup C, Wettergren A, Ugianskis A, Nygaard J, Holte K, Bardram L, Sall M and Mortensen MB: Long-term outcome after early repair of iatrogenic bile duct injury. A national Danish multicentre study. HPB (Oxford). 17:394–400. 2015.PubMed/NCBI View Article : Google Scholar

5 

Tocchi A, Mazzoni G, Liotta G, Lepre L, Cassini D and Miccini M: Late development of bile duct cancer in patients who had biliary-enteric drainage for benign disease: A follow-up study of more than 1,000 patients. Ann Surg. 234:210–214. 2001.PubMed/NCBI View Article : Google Scholar

6 

Buisson EM, Jeong J, Kim HJ and Choi D: Regenerative medicine of the bile duct: Beyond the myth. Int J Stem Cells. 12:183–194. 2019.PubMed/NCBI View Article : Google Scholar

7 

Sampaziotis F, Justin AW, Tysoe OC, Sawiak S, Godfrey EM, Upponi SS, Gieseck RL III, de Brito MC, Berntsen NL, Gómez-Vázquez MJ, et al: Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids. Nat Med. 23:954–963. 2017.PubMed/NCBI View Article : Google Scholar

8 

Justin AW, Saeb-Parsy K, Markaki AE, Vallier L and Sampaziotis F: Advances in the generation of bioengineered bile ducts. Biochim Biophys Acta Mol Basis Dis. 1864:1532–1538. 2018.PubMed/NCBI View Article : Google Scholar

9 

Miyazawa M, Aikawa M, Okada K, Toshimitsu Y, Okamoto K, Koyama I and Ikada Y: Regeneration of extrahepatic bile ducts by tissue engineering with a bioabsorbable polymer. J Artif Organs. 15:26–31. 2012.PubMed/NCBI View Article : Google Scholar

10 

Zeng J, Wang J, Dong J, Huang X, Xia H and Xiang X: The application of vascularized stomach flap to repair postoperative biliary stricture. Medicine (Baltimore). 97(e11344)2018.PubMed/NCBI View Article : Google Scholar

11 

de Jong IEM, Sutton ME, van den Heuvel MC, Gouw ASH and Porte RJ: Evidence for recipient-derived cells in peribiliary glands and biliary epithelium of the large donor bile ducts after liver transplantation. Front Cell Dev Biol. 8(693)2020.PubMed/NCBI View Article : Google Scholar

12 

Lee J, Chan MC, James C and Lantis JC II: Cryopreserved allograft use in vascular surgery. Surg Technol Int. 37:237–243. 2020.PubMed/NCBI

13 

Furlough CL, Jain AK, Ho KJ, Rodriguez HE, Tomita TM and Eskandari MK: Peripheral artery reconstructions using cryopreserved arterial allografts in infected fields. J Vasc Surg. 70:562–568. 2019.PubMed/NCBI View Article : Google Scholar

14 

Heinrich S, Georgiev P, Weber A, Vergopoulos A, Graf R and Clavien PA: Partial bile duct ligation in mice: A novel model of acute cholestasis. Surgery. 149:445–451. 2011.PubMed/NCBI View Article : Google Scholar

15 

Talbot SR, Biernot S, Bleich A, van Dijk RM, Ernst L, Häger C, Helgers SOA, Koegel B, Koska I, Kuhla A, et al: Defining body-weight reduction as a humane endpoint: A critical appraisal. Lab Anim. 54:99–110. 2020.PubMed/NCBI View Article : Google Scholar

16 

Emond J, Capron-Laudereau M, Meriggi F, Bernuau J, Reynes M and Houssin D: Extent of hepatectomy in the rat. Evaluation of basal conditions and effect of therapy. Eur Surg Res. 21:251–259. 1989.PubMed/NCBI View Article : Google Scholar

17 

Chilvery S, Bansod S, Saifi MA and Godugu C: Piperlongumine attenuates bile duct ligation-induced liver fibrosis in mice via inhibition of TGF-β1/Smad and EMT pathways. Int Immunopharmacol. 88(106909)2020.PubMed/NCBI View Article : Google Scholar

18 

Tang G, Seume N, Häger C, Kumstel S, Abshagen K, Bleich A, Vollmar B, Talbot SR, Zhang X and Zechner D: Comparing distress of mouse models for liver damage. Sci Rep. 10(19814)2020.PubMed/NCBI View Article : Google Scholar

19 

Gäbele E, Froh M, Arteel GE, Uesugi T, Hellerbrand C, Schölmerich J, Brenner DA, Thurman RG and Rippe RA: TNFalpha is required for cholestasis-induced liver fibrosis in the mouse. Biochem Biophys Res Commun. 378:348–353. 2009.PubMed/NCBI View Article : Google Scholar

20 

Ezure T, Sakamoto T, Tsuji H, Lunz JG III, Murase N, Fung JJ and Demetris AJ: The development and compensation of biliary cirrhosis in interleukin-6-deficient mice. Am J Pathol. 156:1627–1639. 2000.PubMed/NCBI View Article : Google Scholar

21 

Aikawa M, Miyazawa M, Okamoto K, Toshimitsu Y, Torii T, Okada K, Akimoto N, Ohtani Y, Koyama I and Yoshito I: A novel treatment for bile duct injury with a tissue-engineered bioabsorbable polymer patch. Surgery. 147:575–580. 2010.PubMed/NCBI View Article : Google Scholar

22 

Zong C, Wang M, Yang F, Chen G, Chen J, Tang Z, Liu Q, Gao C, Ma L and Wang J: A novel therapy strategy for bile duct repair using tissue engineering technique: PCL/PLGA bilayered scaffold with hMSCs. J Tissue Eng Regen Med. 11:966–976. 2017.PubMed/NCBI View Article : Google Scholar

23 

Siqueira OH, Herani Filho B, Paula RE, Ascoli FO, Nóbrega AC, Carvalho AC, Pires AR, Gaglionone NC, Cunha KS and Granjeiro JM: Tamoxifen decreases the myofibroblast count in the healing bile duct tissue of pigs. Clinics (Sao Paulo). 68:101–106. 2013.PubMed/NCBI View Article : Google Scholar

24 

Kosmidis C, Efthimiadis C, Anthimidis G, Basdanis G, Apostolidis S, Hytiroglou P, Vasiliadou K, Prousalidis J and Fahantidis E: Myofibroblasts and colonic anastomosis healing in Wistar rats. BMC Surg. 11(6)2011.PubMed/NCBI View Article : Google Scholar

25 

Guruswamy Damodaran R and Vermette P: Tissue and organ decellularization in regenerative medicine. Biotechnol Prog. 34:1494–1505. 2018.PubMed/NCBI View Article : Google Scholar

26 

Roelofs LA, Oosterwijk E, Kortmann BB, Daamen WF, Tiemessen DM, Brouwer KM, Eggink AJ, Crevels AJ, Wijnen RM, van Kuppevelt TH, et al: Bladder regeneration using a smart acellular collagen scaffold with growth factors VEGF, FGF2 and HB-EGF. Tissue Eng Part A. 22:83–92. 2016.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Ge X, Xu D, Pan K, Mao G, Lu S and Li C: Establishment of a mouse model for bile duct repair and tissue engineering. Exp Ther Med 24: 739, 2022.
APA
Ge, X., Xu, D., Pan, K., Mao, G., Lu, S., & Li, C. (2022). Establishment of a mouse model for bile duct repair and tissue engineering. Experimental and Therapeutic Medicine, 24, 739. https://doi.org/10.3892/etm.2022.11675
MLA
Ge, X., Xu, D., Pan, K., Mao, G., Lu, S., Li, C."Establishment of a mouse model for bile duct repair and tissue engineering". Experimental and Therapeutic Medicine 24.6 (2022): 739.
Chicago
Ge, X., Xu, D., Pan, K., Mao, G., Lu, S., Li, C."Establishment of a mouse model for bile duct repair and tissue engineering". Experimental and Therapeutic Medicine 24, no. 6 (2022): 739. https://doi.org/10.3892/etm.2022.11675
Copy and paste a formatted citation
x
Spandidos Publications style
Ge X, Xu D, Pan K, Mao G, Lu S and Li C: Establishment of a mouse model for bile duct repair and tissue engineering. Exp Ther Med 24: 739, 2022.
APA
Ge, X., Xu, D., Pan, K., Mao, G., Lu, S., & Li, C. (2022). Establishment of a mouse model for bile duct repair and tissue engineering. Experimental and Therapeutic Medicine, 24, 739. https://doi.org/10.3892/etm.2022.11675
MLA
Ge, X., Xu, D., Pan, K., Mao, G., Lu, S., Li, C."Establishment of a mouse model for bile duct repair and tissue engineering". Experimental and Therapeutic Medicine 24.6 (2022): 739.
Chicago
Ge, X., Xu, D., Pan, K., Mao, G., Lu, S., Li, C."Establishment of a mouse model for bile duct repair and tissue engineering". Experimental and Therapeutic Medicine 24, no. 6 (2022): 739. https://doi.org/10.3892/etm.2022.11675
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