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Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition

  • Authors:
    • Weitian Wei
    • Jiangfeng Wang
    • Yuqian Hu
    • Sheng Chen
    • Jinshi Liu
  • View Affiliations / Copyright

    Affiliations: The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Cancer and Basic Medicine, Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 7
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    Published online on: November 16, 2022
       https://doi.org/10.3892/etm.2022.11706
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Abstract

The present study aimed to explore the effects and underlying mechanisms of emodin (Emo) on gemcitabine (GEM)‑resistant pancreatic cancer. GEM‑resistant SW1990 cells (SW1990/GZ) were established by successively doubling the concentration of GEM. Cell viability was measured using the CCK‑8 assay and flow cytometry was used to measure cell apoptosis. Cell migration was assessed using a Transwell assay. Sphere and colony‑formation assays were used to evaluate cell self‑renewal. The expression levels of epithelial‑mesenchymal transition (EMT) and stem cell biomarkers were determined using western blotting. Snail family transcriptional repressor 1 gene (Snail) was overexpressed by transfecting cells with pcDNA3.1‑Snail plasmids. A xenograft model was established in nude mice by using SW1990/GZ and Snail‑overexpressing SW1990/GZ cells. Proliferation, migration, self‑renewal and EMT progression of GEM‑treated SW1990/GZ cells were significantly suppressed in vitro by Emo treatment, whereas the overexpression of Snail abolished the aforementioned effects. In in vivo, the antitumor activity of GEM and the inhibitory effect of GEM against EMT progression and stem‑like characteristics were enhanced by treatment with Emo, whilst overexpression of Snail reversed these effects. In conclusion, Emo reversed GEM resistance in pancreatic cancer by suppressing stemness and regulating EMT progression.
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Copy and paste a formatted citation
Spandidos Publications style
Wei W, Wang J, Hu Y, Chen S and Liu J: Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition. Exp Ther Med 25: 7, 2023.
APA
Wei, W., Wang, J., Hu, Y., Chen, S., & Liu, J. (2023). Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition. Experimental and Therapeutic Medicine, 25, 7. https://doi.org/10.3892/etm.2022.11706
MLA
Wei, W., Wang, J., Hu, Y., Chen, S., Liu, J."Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition". Experimental and Therapeutic Medicine 25.1 (2023): 7.
Chicago
Wei, W., Wang, J., Hu, Y., Chen, S., Liu, J."Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition". Experimental and Therapeutic Medicine 25, no. 1 (2023): 7. https://doi.org/10.3892/etm.2022.11706
Copy and paste a formatted citation
x
Spandidos Publications style
Wei W, Wang J, Hu Y, Chen S and Liu J: Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition. Exp Ther Med 25: 7, 2023.
APA
Wei, W., Wang, J., Hu, Y., Chen, S., & Liu, J. (2023). Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition. Experimental and Therapeutic Medicine, 25, 7. https://doi.org/10.3892/etm.2022.11706
MLA
Wei, W., Wang, J., Hu, Y., Chen, S., Liu, J."Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition". Experimental and Therapeutic Medicine 25.1 (2023): 7.
Chicago
Wei, W., Wang, J., Hu, Y., Chen, S., Liu, J."Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition". Experimental and Therapeutic Medicine 25, no. 1 (2023): 7. https://doi.org/10.3892/etm.2022.11706
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