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Article

YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation

  • Authors:
    • Qiang Wang
    • Wei Fan
    • Bingxue Liang
    • Bowen Hou
    • Zaiqun Jiang
    • Chao Li
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Article Number: 37
    |
    Published online on: November 29, 2022
       https://doi.org/10.3892/etm.2022.11736
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Abstract

The present study investigated the effect of transcription factor yin yang 1 (YY1) on aerobic glycolysis and cell proliferation in neuroblastoma and its mechanism. Neuroblastoma cell lines were used to investigate the association between YY1 and lactate dehydrogenase A (LDHA) expression. Cell Counting Kit (CCK)‑8 and clone formation experiments were used to detect the cell viability. The interaction of YY1 and LDHA was detected using chromatin immunoprecipitation assay. Glucose uptake, intra/extracellular lactate and pyruvate and LDHA expression were evaluated using standard methods. Reverse transcription quantitative PCR, western blotting and gene overexpression or silencing were undertaken to explore the biological effects and underlying mechanisms of transcriptional regulators in NB cells. The results demonstrated that YY1 was significantly upregulated in neuroblastoma cell lines. The results of aerobic glycolysis and CCK‑8 indicated that YY1 significantly promoted the proliferation and aerobic glycolysis of neuroblastoma cells. In addition, chromatin immunoprecipitation‑PCR results demonstrated that YY1 was directly bound to the promoter LDHA. Overexpression of LDHA could reverse the inhibitory effect of sh‑YY1 on aerobic glycolysis and proliferation of neuroblastoma cells. In conclusion, YY1 could induce aerobic glycolysis and proliferation of neuroblastoma cell lines, and may directly mediate the regulation of LDHA. These findings may provide novel insight for the treatment of neuroblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Q, Fan W, Liang B, Hou B, Jiang Z and Li C: YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation. Exp Ther Med 25: 37, 2023.
APA
Wang, Q., Fan, W., Liang, B., Hou, B., Jiang, Z., & Li, C. (2023). YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation. Experimental and Therapeutic Medicine, 25, 37. https://doi.org/10.3892/etm.2022.11736
MLA
Wang, Q., Fan, W., Liang, B., Hou, B., Jiang, Z., Li, C."YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation". Experimental and Therapeutic Medicine 25.1 (2023): 37.
Chicago
Wang, Q., Fan, W., Liang, B., Hou, B., Jiang, Z., Li, C."YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation". Experimental and Therapeutic Medicine 25, no. 1 (2023): 37. https://doi.org/10.3892/etm.2022.11736
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Q, Fan W, Liang B, Hou B, Jiang Z and Li C: YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation. Exp Ther Med 25: 37, 2023.
APA
Wang, Q., Fan, W., Liang, B., Hou, B., Jiang, Z., & Li, C. (2023). YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation. Experimental and Therapeutic Medicine, 25, 37. https://doi.org/10.3892/etm.2022.11736
MLA
Wang, Q., Fan, W., Liang, B., Hou, B., Jiang, Z., Li, C."YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation". Experimental and Therapeutic Medicine 25.1 (2023): 37.
Chicago
Wang, Q., Fan, W., Liang, B., Hou, B., Jiang, Z., Li, C."YY1 transcription factor induces proliferation and aerobic glycolysis of neuroblastoma cells via LDHA regulation". Experimental and Therapeutic Medicine 25, no. 1 (2023): 37. https://doi.org/10.3892/etm.2022.11736
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