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Polygonum tinctorium leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice

  • Authors:
    • Shogo Kawaguchi
    • Hirotake Sakuraba
    • Hidezumi Kikuchi
    • Kota Matsuki
    • Yudai Hayashi
    • Jiangli Ding
    • Yusuke Tanaka
    • Kazuhiko Seya
    • Tomoh Matsumiya
    • Hiroto Hiraga
    • Shinsaku Fukuda
    • Kenroh Sasaki
    • Tadaatsu Imaizumi
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036-8562, Japan, Department of Gastroenterology and Hematology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036-8562, Japan, Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036-8562, Japan, Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Science, Hirosaki, Aomori 036-8564, Japan, Division of Pharmacognosy, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi 981-8558, Japan
    Copyright: © Kawaguchi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 112
    |
    Published online on: January 26, 2023
       https://doi.org/10.3892/etm.2023.11811
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Abstract

Dietary fat strongly influences the intestinal mucosal barrier, which protects against invading pathogenic bacteria. A high-fat diet (HFD) compromises the integrity of epithelial tight junctions (TJs) and reduces mucin production, leading to intestinal barrier disruption and metabolic endotoxemia. It has been shown that the active constituents of indigo plants can protect against intestinal inflammation; however, their protective role in HFD-induced intestinal epithelial damage remains unknown. The present study aimed to investigate the effects of Polygonum tinctorium leaf extract (indigo Ex) on HFD-induced intestinal damage in mice. Male C57BL6/J mice were fed a HFD and injected intraperitoneally with either indigo Ex or phosphate-buffered saline (PBS) for 4 weeks. The expression levels of TJ proteins, zonula occludens-1 and Claudin-1, were analyzed by immunofluorescence staining and western blotting. The colon mRNA expression levels of tumor necrosis factor-α, interleukin (IL)-12p40, IL-10 and IL-22 were measured by reverse transcription-quantitative PCR. The results revealed that indigo Ex administration attenuated the HFD-induced shortening of the colon. Colon crypt length was shown to be significantly greater in the indigo Ex-treated group mice compared with that in the PBS-treated group mice. Moreover, indigo Ex administration increased the number of goblet cells, and ameliorated the redistribution of TJ proteins. Notably, indigo Ex significantly increased the colon mRNA expression levels of IL-10. Indigo Ex displayed little effect on the gut microbial composition of HFD-fed mice. Taken together, these results suggested that indigo Ex may protect against HFD-induced epithelial damage. The leaves of indigo plants contain promising natural therapeutic compounds that could be used to treat obesity-associated intestinal damage and metabolic inflammation.
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Copy and paste a formatted citation
Spandidos Publications style
Kawaguchi S, Sakuraba H, Kikuchi H, Matsuki K, Hayashi Y, Ding J, Tanaka Y, Seya K, Matsumiya T, Hiraga H, Hiraga H, et al: <em>Polygonum tinctorium</em> leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice. Exp Ther Med 25: 112, 2023.
APA
Kawaguchi, S., Sakuraba, H., Kikuchi, H., Matsuki, K., Hayashi, Y., Ding, J. ... Imaizumi, T. (2023). <em>Polygonum tinctorium</em> leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice. Experimental and Therapeutic Medicine, 25, 112. https://doi.org/10.3892/etm.2023.11811
MLA
Kawaguchi, S., Sakuraba, H., Kikuchi, H., Matsuki, K., Hayashi, Y., Ding, J., Tanaka, Y., Seya, K., Matsumiya, T., Hiraga, H., Fukuda, S., Sasaki, K., Imaizumi, T."<em>Polygonum tinctorium</em> leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice". Experimental and Therapeutic Medicine 25.3 (2023): 112.
Chicago
Kawaguchi, S., Sakuraba, H., Kikuchi, H., Matsuki, K., Hayashi, Y., Ding, J., Tanaka, Y., Seya, K., Matsumiya, T., Hiraga, H., Fukuda, S., Sasaki, K., Imaizumi, T."<em>Polygonum tinctorium</em> leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice". Experimental and Therapeutic Medicine 25, no. 3 (2023): 112. https://doi.org/10.3892/etm.2023.11811
Copy and paste a formatted citation
x
Spandidos Publications style
Kawaguchi S, Sakuraba H, Kikuchi H, Matsuki K, Hayashi Y, Ding J, Tanaka Y, Seya K, Matsumiya T, Hiraga H, Hiraga H, et al: <em>Polygonum tinctorium</em> leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice. Exp Ther Med 25: 112, 2023.
APA
Kawaguchi, S., Sakuraba, H., Kikuchi, H., Matsuki, K., Hayashi, Y., Ding, J. ... Imaizumi, T. (2023). <em>Polygonum tinctorium</em> leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice. Experimental and Therapeutic Medicine, 25, 112. https://doi.org/10.3892/etm.2023.11811
MLA
Kawaguchi, S., Sakuraba, H., Kikuchi, H., Matsuki, K., Hayashi, Y., Ding, J., Tanaka, Y., Seya, K., Matsumiya, T., Hiraga, H., Fukuda, S., Sasaki, K., Imaizumi, T."<em>Polygonum tinctorium</em> leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice". Experimental and Therapeutic Medicine 25.3 (2023): 112.
Chicago
Kawaguchi, S., Sakuraba, H., Kikuchi, H., Matsuki, K., Hayashi, Y., Ding, J., Tanaka, Y., Seya, K., Matsumiya, T., Hiraga, H., Fukuda, S., Sasaki, K., Imaizumi, T."<em>Polygonum tinctorium</em> leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice". Experimental and Therapeutic Medicine 25, no. 3 (2023): 112. https://doi.org/10.3892/etm.2023.11811
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