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MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3

  • Authors:
    • Ju-Yeon Kim
    • Eun Jung Jung
    • Jae-Myung Kim
    • Youngsim Son
    • Han Shine Lee
    • Seung-Jin Kwag
    • Ji-Ho Park
    • Jin-Kyu Cho
    • Han-Gil Kim
    • Taejin Park
    • Sang-Ho Jeong
    • Chi-Young Jeong
    • Young-Tae Ju
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Jinju, Gyeongsang 52727, Republic of Korea, Department of Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Changwon Hospital, Changwon, Gyeongsang 51472, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 127
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    Published online on: February 7, 2023
       https://doi.org/10.3892/etm.2023.11826
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Abstract

Breast malignancy remains one of the most common causes of cancer‑associated mortalities among women. MicroRNA (miR)‑221 and miR‑222 are homologous miRs and have a substantial impact on cancer progression. In the present study, the regulatory mechanisms of miR‑221/222 and its target annexin A3 (ANXA3) in breast cancer cells were investigated. Breast tissue samples were collected to evaluate the expression patterns of miR‑221/222 levels in breast cancer cell lines and cancer tissues according to clinical characteristics. The levels of miR‑221/222 were increased or decreased in cancer cell lines compared with normal breast cell lines according to cell line subtype. Subsequently, the changes in the progression and invasion of breast cancer cells were investigated using cell proliferation, invasion assay, gap closure and colony formation assays. Western blotting of cell cycle proteins and flow cytometry were performed to evaluate the possible pathway of miR‑221/222 and ANXA3 axis. Chemosensitivity tests were performed to explore the suitability of the miR‑221/222 and ANXA3 axis as a therapeutic target in breast cancer. The expression levels of miR‑221/222 were associated with aggressive characteristics of breast cancer subtypes. Cell transfection assay demonstrated the regulation of breast cancer proliferation and invasiveness by miR‑221/222. MiR‑221/222 directly targeted the 3'‑untranslated region of ANXA3 and suppressed the expression of ANXA3 at the mRNA and protein levels. In addition, miR‑221/222 negatively regulated cell proliferation and the cell cycle pathway in breast cancer cells by targeting ANXA3. In combination with adriamycin, downregulation of ANXA3 may sensitize adriamycin‑induced cell death to induction of persistent G2/M and G0/G1 arrest. Decreased expression of ANXA3 through increased expression of miR‑221/222 reduced breast cancer progression and increased the effectiveness of the chemotherapy drug. The present results indicated the miR‑221/222 and ANXA3 axis to be a possible novel therapeutic target for the treatment of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Kim J, Jung E, Kim J, Son Y, Lee H, Kwag S, Park J, Cho J, Kim H, Park T, Park T, et al: MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3. Exp Ther Med 25: 127, 2023.
APA
Kim, J., Jung, E., Kim, J., Son, Y., Lee, H., Kwag, S. ... Ju, Y. (2023). MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3. Experimental and Therapeutic Medicine, 25, 127. https://doi.org/10.3892/etm.2023.11826
MLA
Kim, J., Jung, E., Kim, J., Son, Y., Lee, H., Kwag, S., Park, J., Cho, J., Kim, H., Park, T., Jeong, S., Jeong, C., Ju, Y."MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3". Experimental and Therapeutic Medicine 25.3 (2023): 127.
Chicago
Kim, J., Jung, E., Kim, J., Son, Y., Lee, H., Kwag, S., Park, J., Cho, J., Kim, H., Park, T., Jeong, S., Jeong, C., Ju, Y."MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3". Experimental and Therapeutic Medicine 25, no. 3 (2023): 127. https://doi.org/10.3892/etm.2023.11826
Copy and paste a formatted citation
x
Spandidos Publications style
Kim J, Jung E, Kim J, Son Y, Lee H, Kwag S, Park J, Cho J, Kim H, Park T, Park T, et al: MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3. Exp Ther Med 25: 127, 2023.
APA
Kim, J., Jung, E., Kim, J., Son, Y., Lee, H., Kwag, S. ... Ju, Y. (2023). MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3. Experimental and Therapeutic Medicine, 25, 127. https://doi.org/10.3892/etm.2023.11826
MLA
Kim, J., Jung, E., Kim, J., Son, Y., Lee, H., Kwag, S., Park, J., Cho, J., Kim, H., Park, T., Jeong, S., Jeong, C., Ju, Y."MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3". Experimental and Therapeutic Medicine 25.3 (2023): 127.
Chicago
Kim, J., Jung, E., Kim, J., Son, Y., Lee, H., Kwag, S., Park, J., Cho, J., Kim, H., Park, T., Jeong, S., Jeong, C., Ju, Y."MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3". Experimental and Therapeutic Medicine 25, no. 3 (2023): 127. https://doi.org/10.3892/etm.2023.11826
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