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Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts

  • Authors:
    • Zhen Cao
    • Zhixiong Yan
    • Jiahui Wang
    • Huan Yang
    • Biao Han
    • Jintao Gao
    • Yong Guo
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Engineering, College of Biotechnology, Guilin Medical University, Guilin, Guangxi 541199, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 138
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    Published online on: February 13, 2023
       https://doi.org/10.3892/etm.2023.11837
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Abstract

Bone is the main site of metastasis from prostate cancer; therefore, it is important to investigate the microRNAs (miRNAs) and mRNA associated with bone metastases from prostate cancer. Since an appropriate mechanical environment is important in the growth of bone, in the present study, the miRNA, mRNA, and long non‑coding RNA (lncRNA) profiles of mechanically strained osteoblasts treated with conditioned medium (CM) from PC‑3 prostate cancer cells were studied. MC3T3‑E1 osteoblastic cells were treated with the CM of PC‑3 prostate cancer cells and were simultaneously stimulated with a mechanical tensile strain of 2,500 µε at 0.5 Hz; the osteoblastic differentiation of the MC3T3‑E1 cells was then assessed. In addition, the differential expression levels of mRNA, miRNA and lncRNA in MC3T3‑E1 cells treated with the CM of PC‑3 cells were screened, and some of the miRNAs and mRNAs were verified by reverse transcription‑quantitative PCR (RT‑qPCR). The signal molecules and signaling pathways associated with osteogenic differentiation were predicted by bioinformatics analysis. The CM of PC‑3 prostate cancer cells suppressed osteoblastic differentiation of MC3T3‑E1 cells. A total of seven upregulated miRNAs and 12 downregulated miRNAs were selected by sequencing and further verified using RT‑qPCR, and related differentially expressed genes (11 upregulated and 12 downregulated genes) were also selected by sequencing and further verified using RT‑qPCR; subsequently, according to the enrichment of differentially expressed genes in signaling pathways, nine signaling pathways involved in osteogenic differentiation were screened out. Furthermore, a functional mRNA‑miRNA‑lncRNA regulatory network was constructed. The differentially expressed miRNAs, mRNAs and lncRNAs may provide a novel signature in bone metastases of prostate cancer. Notably, some of the signaling pathways and related genes may be associated with pathological osteogenic differentiation caused by bone metastasis of prostate cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Cao Z, Yan Z, Wang J, Yang H, Han B, Gao J and Guo Y: Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts. Exp Ther Med 25: 138, 2023.
APA
Cao, Z., Yan, Z., Wang, J., Yang, H., Han, B., Gao, J., & Guo, Y. (2023). Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts. Experimental and Therapeutic Medicine, 25, 138. https://doi.org/10.3892/etm.2023.11837
MLA
Cao, Z., Yan, Z., Wang, J., Yang, H., Han, B., Gao, J., Guo, Y."Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts". Experimental and Therapeutic Medicine 25.3 (2023): 138.
Chicago
Cao, Z., Yan, Z., Wang, J., Yang, H., Han, B., Gao, J., Guo, Y."Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts". Experimental and Therapeutic Medicine 25, no. 3 (2023): 138. https://doi.org/10.3892/etm.2023.11837
Copy and paste a formatted citation
x
Spandidos Publications style
Cao Z, Yan Z, Wang J, Yang H, Han B, Gao J and Guo Y: Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts. Exp Ther Med 25: 138, 2023.
APA
Cao, Z., Yan, Z., Wang, J., Yang, H., Han, B., Gao, J., & Guo, Y. (2023). Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts. Experimental and Therapeutic Medicine, 25, 138. https://doi.org/10.3892/etm.2023.11837
MLA
Cao, Z., Yan, Z., Wang, J., Yang, H., Han, B., Gao, J., Guo, Y."Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts". Experimental and Therapeutic Medicine 25.3 (2023): 138.
Chicago
Cao, Z., Yan, Z., Wang, J., Yang, H., Han, B., Gao, J., Guo, Y."Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts". Experimental and Therapeutic Medicine 25, no. 3 (2023): 138. https://doi.org/10.3892/etm.2023.11837
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