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A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression

  • Authors:
    • Yanbin Zheng
    • Shenglan Wang
    • Yutong Zhong
    • Chunhong Huang
    • Xinjie Wu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, LongYan First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 364000, P.R. China, Emergency Department, LongYan People Hospital of Fujian, Longyan, Fujian 364000, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 147
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    Published online on: February 15, 2023
       https://doi.org/10.3892/etm.2023.11846
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Abstract

Breast cancer is the most common malignant tumor in females, and the majority of patients succumb to metastasis. The present study aimed to investigate the association between tumor necrosis factor alpha‑induced protein 3 (A20), NOD‑, LRR‑ and pyrin domain‑containing protein 3 (NLRP3) and tumor‑associated macrophage polarization, and their effects on the proliferation and metastasis of breast cancer cells. The expression of A20 in breast cancer cells was analyzed by reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting. RT‑qPCR and western blotting were also used to confirm the transfection efficiency. The viability, clone formation, migration, invasion and angiogenesis of transfected breast cancer cells were detected by Cell Counting Kit‑8, colony formation, wound healing, Transwell and tube formation assays, respectively. Activated macrophages, namely M1 and M2 type macrophages, were observed by double staining immunofluorescence. The levels of M1 and M2 macrophage markers were analyzed by qPCR. The expression of angiogenesis‑related proteins and NLRP3 inflammasome activation‑associated proteins was detected by western blotting. The results revealed that A20 was highly expressed in breast cancer cells. Interference with A20 inhibited the proliferation, invasion, migration and angiogenesis of breast cancer cells, and inhibited the M2‑like polarization of macrophages. Interference with A20 promoted the activation of the NLRP3 inflammasome. The NLRP3 inhibitor MCC950 alleviated the effect of interference with A20 to promote macrophage proliferation and recruitment, as well as M2‑like polarization. In conclusion, interference with A20 inhibited macrophage proliferation and M2‑like polarization through the NLRP3 inflammasome signaling pathway to inhibit breast cancer progression.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng Y, Wang S, Zhong Y, Huang C and Wu X: A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression. Exp Ther Med 25: 147, 2023.
APA
Zheng, Y., Wang, S., Zhong, Y., Huang, C., & Wu, X. (2023). A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression. Experimental and Therapeutic Medicine, 25, 147. https://doi.org/10.3892/etm.2023.11846
MLA
Zheng, Y., Wang, S., Zhong, Y., Huang, C., Wu, X."A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression". Experimental and Therapeutic Medicine 25.4 (2023): 147.
Chicago
Zheng, Y., Wang, S., Zhong, Y., Huang, C., Wu, X."A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression". Experimental and Therapeutic Medicine 25, no. 4 (2023): 147. https://doi.org/10.3892/etm.2023.11846
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng Y, Wang S, Zhong Y, Huang C and Wu X: A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression. Exp Ther Med 25: 147, 2023.
APA
Zheng, Y., Wang, S., Zhong, Y., Huang, C., & Wu, X. (2023). A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression. Experimental and Therapeutic Medicine, 25, 147. https://doi.org/10.3892/etm.2023.11846
MLA
Zheng, Y., Wang, S., Zhong, Y., Huang, C., Wu, X."A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression". Experimental and Therapeutic Medicine 25.4 (2023): 147.
Chicago
Zheng, Y., Wang, S., Zhong, Y., Huang, C., Wu, X."A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression". Experimental and Therapeutic Medicine 25, no. 4 (2023): 147. https://doi.org/10.3892/etm.2023.11846
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