Open Access

Exosomal miR‑152‑5p/ARHGAP6/ROCK axis regulates apoptosis and fibrosis in cardiomyocytes

  • Authors:
    • Shaoyuan Chen
    • Yulang Huang
    • Rongzhi Liu
    • Zixiang Lin
    • Bihan Huang
    • Wen Ai
    • Jianjun He
    • Yulan Gao
    • Peiyi Xie
  • View Affiliations

  • Published online on: February 28, 2023     https://doi.org/10.3892/etm.2023.11864
  • Article Number: 165
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute myocardial infarction (AMI) is a fatal cardiovascular disease with a high mortality rate. The discovery of effective biomarkers is crucial for the diagnosis and treatment of AMI. In the present study, miRNA sequencing and reverse transcription‑quantitative polymerase chain reaction techniques revealed that the expression of exosome derived miR‑152‑5p was significantly downregulated in patients with AMI compared with healthy controls. A series of functional validation experiments were then performed using H9c2 cardiomyocytes. Following transfection of the cardiomyocytes using an miR‑152‑5p inhibitor, immunofluorescence staining of a‑smooth muscle actin revealed a marked increase in fibrosis. Western blotting revealed that the expression levels of the apoptotic protein Bax, TNF‑α and collagen‑associated proteins were significantly increased, whereas those of the apoptosis‑inhibiting factor Bcl‑2 and vascular endothelial growth factor A were significantly decreased. Furthermore, the binding of Rho GTPase‑activating protein 6 (ARHGAP6) to miR‑152‑5p was predicted using an online database and verified using a dual‑luciferase reporter gene assay. The transfection of cardiomyocytes with miR‑152‑5p mimics was found to inhibit the activation of ARHGAP6 and Rho‑associated coiled‑coil containing kinase 2 (ROCK2). These results suggest that miR‑152‑5p targets ARHGAP6 through the ROCK signaling pathway to inhibit AMI, which implies that miR‑152‑5p may be a diagnostic indicator and potential target for treatment of myocardial infarction.
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April-2023
Volume 25 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen S, Huang Y, Liu R, Lin Z, Huang B, Ai W, He J, Gao Y and Xie P: Exosomal miR‑152‑5p/ARHGAP6/ROCK axis regulates apoptosis and fibrosis in cardiomyocytes. Exp Ther Med 25: 165, 2023
APA
Chen, S., Huang, Y., Liu, R., Lin, Z., Huang, B., Ai, W. ... Xie, P. (2023). Exosomal miR‑152‑5p/ARHGAP6/ROCK axis regulates apoptosis and fibrosis in cardiomyocytes. Experimental and Therapeutic Medicine, 25, 165. https://doi.org/10.3892/etm.2023.11864
MLA
Chen, S., Huang, Y., Liu, R., Lin, Z., Huang, B., Ai, W., He, J., Gao, Y., Xie, P."Exosomal miR‑152‑5p/ARHGAP6/ROCK axis regulates apoptosis and fibrosis in cardiomyocytes". Experimental and Therapeutic Medicine 25.4 (2023): 165.
Chicago
Chen, S., Huang, Y., Liu, R., Lin, Z., Huang, B., Ai, W., He, J., Gao, Y., Xie, P."Exosomal miR‑152‑5p/ARHGAP6/ROCK axis regulates apoptosis and fibrosis in cardiomyocytes". Experimental and Therapeutic Medicine 25, no. 4 (2023): 165. https://doi.org/10.3892/etm.2023.11864