Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
April-2023 Volume 25 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2023 Volume 25 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway

  • Authors:
    • Juan Gao
    • Shiqi Suo
    • Jingxia Li
    • Chunxia Wang
    • Ranran Deng
    • Yuxia Hu
    • Chunzheng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Affiliated Hospital of Hebei Engineering University, Handan, Hebei 056001, P.R. China, Department of Gynecology, Affiliated Hospital of Hebei Engineering University, Handan, Hebei 056001, P.R. China, Department of Obstetrics, Handan First Hospital, Handan, Hebei 056002, P.R. China, Department of Functional Section I, Affiliated Hospital of Hebei Engineering University, Handan, Hebei 056000, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 169
    |
    Published online on: March 3, 2023
       https://doi.org/10.3892/etm.2023.11868
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Insulin‑like growth factor binding protein‑related protein 1 (IGFBP‑rP1) is a potential tumor suppressor gene in a variety of cancers including colorectal cancer and breast cancer. However, its role and the potential mechanism in endometrial carcinoma (EC) are still unclear. The purpose of this study was to explore the effect of IGFBP‑rP1 on EC cell proliferation and apoptosis and its underlying mechanism. Western blot analysis and reverse transcription‑quantitative PCR were used to assess protein and gene expression levels of IGFBP‑rP1 in EC cells. Overexpression of IGFBP‑rP1 and/or AKT serine/threonine kinase was used to analyze their effects on cell proliferation and apoptosis of the EC cells. Co‑immunoprecipitation and glutathione S transferase pull‑down assays were used to analyze the interaction between IGFBP‑rP1 and AKT. The expression of IGFBP‑rP1 in EC cells was downregulated. Overexpression of IGFBP‑rP1 inhibited the proliferation and induced apoptosis of EC cells, which were abolished by the overexpression of AKT. In addition, IGFBP‑rP1 directly interacted with AKT to inhibit PI3K/AKT signaling. Additionally, M0 macrophages were induced by EC cells to differentiate into M2 macrophages, which was reversed by IGFBP‑rP1. Overexpression of AKT in EC cells abolished the inhibitory effect of IGFBP‑rP1 on M2 polarization. IGFBP‑rP1 as an oncogenic factor inhibits M2 polarization of TAMs through PI3K/AKT signaling pathway and may be a potentially valuable target for EC therapy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Xiao L, He Y, Peng F, Yang J and Yuan C: Endometrial cancer cells promote M2-Like macrophage polarization by delivering exosomal miRNA-21 under hypoxia condition. J Immunol Res. 2020(9731049)2020.PubMed/NCBI View Article : Google Scholar

2 

Braun MM, Overbeek-Wager EA and Grumbo RJ: Diagnosis and management of endometrial cancer. Am Fam Physician. 93:468–474. 2016.PubMed/NCBI

3 

Hinshaw DC and Shevde LA: The tumor microenvironment innately modulates cancer progression. Cancer Res. 79:4557–4566. 2019.PubMed/NCBI View Article : Google Scholar

4 

Vitale I, Manic G, Coussens LM, Kroemer G and Galluzzi L: Macrophages and metabolism in the tumor microenvironment. Cell Metab. 30:36–50. 2019.PubMed/NCBI View Article : Google Scholar

5 

Kim J and Bae JS: Tumor-Associated macrophages and neutrophils in tumor microenvironment. Mediators Inflamm. 2016(6058147)2016.PubMed/NCBI View Article : Google Scholar

6 

Genin M, Clement F, Fattaccioli A, Raes M and Michiels C: M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide. BMC Cancer. 15(577)2015.PubMed/NCBI View Article : Google Scholar

7 

Cassetta L and Pollard JW: Tumor-associated macrophages. Curr Biol. 30:R246–R248. 2020.PubMed/NCBI View Article : Google Scholar

8 

Shapouri-Moghaddam A, Mohammadian S, Vazini H, Taghadosi M, Esmaeili SA, Mardani F, Seifi B, Mohammadi A, Afshari JT and Sahebkar A: Macrophage plasticity, polarization, and function in health and disease. J Cell Physiol. 233:6425–6440. 2018.PubMed/NCBI View Article : Google Scholar

9 

Cersosimo F, Lonardi S, Bernardini G, Telfer B, Mandelli GE, Santucci A, Vermi W and Giurisato E: Tumor-Associated macrophages in osteosarcoma: From mechanisms to therapy. Int J Mol Sci. 21(E5207)2020.PubMed/NCBI View Article : Google Scholar

10 

Wang X, Gao S, Song L, Liu M, Sun Z and Liu J: Astragaloside IV antagonizes M2 phenotype macrophage polarization-evoked ovarian cancer cell malignant progression by suppressing the HMGB1-TLR4 axis. Mol Immunol. 130:113–121. 2021.PubMed/NCBI View Article : Google Scholar

11 

Mohd Nafi SN, Siti Azrin AH, Mat Zin AA, Othman NH and Che Jalil NA: Expression of IGFBP-rP1 in ovarian and breast cancers in association with diabetes mellitus status. Malays J Pathol. 41:33–39. 2019.PubMed/NCBI

12 

Ruan W, Zhu S, Wang H, Xu F, Deng H, Ma Y and Lai M: IGFBP-rP1, a potential molecule associated with colon cancer differentiation. Mol Cancer. 9(281)2010.PubMed/NCBI View Article : Google Scholar

13 

Ma Y, Jiang J, Zhang Y, Ding Y, Xu T and Lu B: IGFBP-rP1 acts as a potential tumor suppressor via the suppression of ERK signaling pathway in endometrial cancer cells. Mol Med Rep. 16:1445–1450. 2017.PubMed/NCBI View Article : Google Scholar

14 

Hu S, Chen R, Man X, Feng X, Cen J, Gu W, He H, Li J, Chai Y and Chen Z: Function and expression of insulin-like growth factor-binding protein 7 (IGFBP7) gene in childhood acute myeloid leukemia. Pediatr Hematol Oncol. 28:279–287. 2011.PubMed/NCBI View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

16 

Ersahin T, Tuncbag N and Cetin-Atalay R: The PI3K/AKT/mTOR interactive pathway. Mol Biosyst. 11:1946–1954. 2015.PubMed/NCBI View Article : Google Scholar

17 

Porta C, Paglino C and Mosca A: Targeting PI3K/Akt/mTOR signaling in cancer. Front Oncol. 4(64)2014.PubMed/NCBI View Article : Google Scholar

18 

Roncolato F, Lindemann K, Willson ML, Martyn J and Mileshkin L: PI3K/AKT/mTOR inhibitors for advanced or recurrent endometrial cancer. Cochrane Database Syst Rev. 10(CD012160)2019.PubMed/NCBI View Article : Google Scholar

19 

Aoki M and Fujishita T: Oncogenic Roles of the PI3K/AKT/mTOR Axis. Curr Top Microbiol Immunol. 407:153–189. 2017.PubMed/NCBI View Article : Google Scholar

20 

Komohara Y and Takeya M: CAFs and TAMs: Maestros of the tumour microenvironment. J Pathol. 241:313–315. 2017.PubMed/NCBI View Article : Google Scholar

21 

Cortés M, Sanchez-Moral L, de Barrios O, Fernández-Aceñero MJ, Martínez-Campanario MC, Esteve-Codina A, Darling DS, Győrffy B, Lawrence T, Dean DC and Postigo A: Tumor-associated macrophages (TAMs) depend on ZEB1 for their cancer-promoting roles. EMBO J. 36:3336–3355. 2017.PubMed/NCBI View Article : Google Scholar

22 

Pan Y, Yu Y, Wang X and Zhang T: Tumor-Associated macrophages in tumor immunity. Front Immunol. 11(583084)2020.PubMed/NCBI View Article : Google Scholar

23 

Ge Z and Ding S: The crosstalk between tumor-associated macrophages (TAMs) and tumor cells and the corresponding targeted therapy. Front Oncol. 10(590941)2020.PubMed/NCBI View Article : Google Scholar

24 

Cassetta L, Fragkogianni S, Sims AH, Swierczak A, Forrester LM, Zhang H, Soong DYH, Cotechini T, Anur P, Lin EY, et al: Human Tumor-Associated macrophage and monocyte transcriptional landscapes reveal cancer-specific reprogramming, biomarkers, and therapeutic targets. Cancer Cell. 35:588–602.e10. 2019.PubMed/NCBI View Article : Google Scholar

25 

Gu S, Ni T, Wang J, Liu Y, Fan Q and Wang Y, Huang T, Chu Y, Sun X and Wang Y: CD47 blockade inhibits tumor progression through promoting phagocytosis of tumor cells by M2 polarized macrophages in endometrial cancer. J Immunol Res. 2018(6156757)2018.PubMed/NCBI View Article : Google Scholar

26 

Giannone G, Ghisoni E, Genta S, Scotto G, Tuninetti V, Turinetto M and Valabrega G: Immuno-Metabolism and microenvironment in cancer: Key players for immunotherapy. Int J Mol Sci. 21(E4414)2020.PubMed/NCBI View Article : Google Scholar

27 

Kowal J, Kornete M and Joyce JA: Re-education of macrophages as a therapeutic strategy in cancer. Immunotherapy. 11:677–689. 2019.PubMed/NCBI View Article : Google Scholar

28 

Damien C, Cisse F, Ligot N, Toure ML, Konaté M, Barry SD, Saw M and Naeije G: Insights in the pathophysiology of haemorrhagic strokes in a sub-Sahara African country, an epidemiological and MRI study. Trop Med Int Health. 26:166–172. 2021.PubMed/NCBI View Article : Google Scholar

29 

Weichhart T, Hengstschläger M and Linke M: Regulation of innate immune cell function by mTOR. Nat Rev Immunol. 15(599614)2015.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gao J, Suo S, Li J, Wang C, Deng R, Hu Y and Zhang C: IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway. Exp Ther Med 25: 169, 2023.
APA
Gao, J., Suo, S., Li, J., Wang, C., Deng, R., Hu, Y., & Zhang, C. (2023). IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway. Experimental and Therapeutic Medicine, 25, 169. https://doi.org/10.3892/etm.2023.11868
MLA
Gao, J., Suo, S., Li, J., Wang, C., Deng, R., Hu, Y., Zhang, C."IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway". Experimental and Therapeutic Medicine 25.4 (2023): 169.
Chicago
Gao, J., Suo, S., Li, J., Wang, C., Deng, R., Hu, Y., Zhang, C."IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway". Experimental and Therapeutic Medicine 25, no. 4 (2023): 169. https://doi.org/10.3892/etm.2023.11868
Copy and paste a formatted citation
x
Spandidos Publications style
Gao J, Suo S, Li J, Wang C, Deng R, Hu Y and Zhang C: IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway. Exp Ther Med 25: 169, 2023.
APA
Gao, J., Suo, S., Li, J., Wang, C., Deng, R., Hu, Y., & Zhang, C. (2023). IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway. Experimental and Therapeutic Medicine, 25, 169. https://doi.org/10.3892/etm.2023.11868
MLA
Gao, J., Suo, S., Li, J., Wang, C., Deng, R., Hu, Y., Zhang, C."IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway". Experimental and Therapeutic Medicine 25.4 (2023): 169.
Chicago
Gao, J., Suo, S., Li, J., Wang, C., Deng, R., Hu, Y., Zhang, C."IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway". Experimental and Therapeutic Medicine 25, no. 4 (2023): 169. https://doi.org/10.3892/etm.2023.11868
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team