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miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis

  • Authors:
    • Jia Nie
    • Wenjing Zhou
    • Shouyang Yu
    • Song Cao
    • Haiying Wang
    • Tian Yu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, P.R. China, Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical University, Zunyi, Guizhou 563000, P.R. China
    Copyright: © Nie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 180
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    Published online on: March 10, 2023
       https://doi.org/10.3892/etm.2023.11879
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Abstract

MicroRNAs (miRNAs or miRs) are commonly involved in regulating myocardial ischemia/reperfusion (I/R) injury by binding and silencing their target genes. However, whether miRNAs regulate myocardial I/R‑induced pyroptosis remains unclear. The present study established an in vivo rat model of myocardial I/R injury and in vitro hypoxia/reoxygenation (H/R) injury model in rat primary cardiomyocytes to investigate the function and the underlying mechanisms of miRNAs on I/R injury‑induced pyroptosis. RNA sequencing was utilized to select the candidate miRNAs between normal and I/R group. Reverse transcription‑quantitative PCR and western blotting were performed to detect candidate miRNAs (miR‑30c‑5p, also known as miR‑30c) and SRY‑related high mobility group‑box gene 9 (SOX9) expression, as well as expression of pyroptosis‑associated proteins (NF‑κB, ASC, caspase‑1, NLRP3) in the myocardial I/R model. ELISA was used to measure pyroptosis‑associated inflammatory markers IL‑18 and IL‑1β. Moreover, the link between miR‑30c and SOX9 was predicted using bioinformatics and luciferase reporter assay. In myocardial I/R injured rats, miR‑30c was downregulated, while the expression of SOX9 was upregulated. Overexpression of miR‑30c inhibited pyroptosis both in vivo and in vitro. Furthermore, miR‑30c negatively regulated SOX9 expression by binding its 3'untranslated region. In conclusion, the miR‑30c/SOX9 axis decreased myocardial I/R injury by suppressing pyroptosis, which may be a potential therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Nie J, Zhou W, Yu S, Cao S, Wang H and Yu T: miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis. Exp Ther Med 25: 180, 2023.
APA
Nie, J., Zhou, W., Yu, S., Cao, S., Wang, H., & Yu, T. (2023). miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis. Experimental and Therapeutic Medicine, 25, 180. https://doi.org/10.3892/etm.2023.11879
MLA
Nie, J., Zhou, W., Yu, S., Cao, S., Wang, H., Yu, T."miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis". Experimental and Therapeutic Medicine 25.4 (2023): 180.
Chicago
Nie, J., Zhou, W., Yu, S., Cao, S., Wang, H., Yu, T."miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis". Experimental and Therapeutic Medicine 25, no. 4 (2023): 180. https://doi.org/10.3892/etm.2023.11879
Copy and paste a formatted citation
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Spandidos Publications style
Nie J, Zhou W, Yu S, Cao S, Wang H and Yu T: miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis. Exp Ther Med 25: 180, 2023.
APA
Nie, J., Zhou, W., Yu, S., Cao, S., Wang, H., & Yu, T. (2023). miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis. Experimental and Therapeutic Medicine, 25, 180. https://doi.org/10.3892/etm.2023.11879
MLA
Nie, J., Zhou, W., Yu, S., Cao, S., Wang, H., Yu, T."miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis". Experimental and Therapeutic Medicine 25.4 (2023): 180.
Chicago
Nie, J., Zhou, W., Yu, S., Cao, S., Wang, H., Yu, T."miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosis". Experimental and Therapeutic Medicine 25, no. 4 (2023): 180. https://doi.org/10.3892/etm.2023.11879
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