Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
May-2023 Volume 25 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2023 Volume 25 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Immunostimulatory and anti‑obesity activities of Adenocaulon himalaicum leaves in RAW264.7 and 3T3‑L1 cells

  • Authors:
    • Hyeok Jin Choi
    • Jeong Won Choi
    • Seung Woo Im
    • Jin Boo Jeong
  • View Affiliations / Copyright

    Affiliations: Department of Forest Science, Andong National University, Andong, North Gyeongsang 36729, Republic of Korea
    Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 219
    |
    Published online on: March 28, 2023
       https://doi.org/10.3892/etm.2023.11918
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study investigated the immunostimulatory activity and anti‑obesity activity of Adenocaulon himalaicum leaf extracts (AHL) in RAW264.7 cells and 3T3‑L1 cells. AHL increased the production of immunostimulatory factors, such as NO, inducible nitric oxide synthase (iNOS), IL‑1β, IL‑6 and TNF‑α and activated the phagocytotic activity in RAW264.7 cells. Inhibition of Toll‑like receptor 4 (TLR4) attenuated the AHL‑mediated production of immunostimulatory factors and activation of phagocytic activity in RAW264.7 cells. Inhibition of p38 and JNK blocked the AHL‑mediated production of immunostimulatory factors, whereas inhibition of TLR4 suppressed the AHL‑mediated phosphorylation of p38 and JNK. Additionally, AHL blocked the lipid accumulation in 3T3‑L1 cells. AHL downregulated proliferator‑activated receptor γ, CCAAT enhancer binding protein α and perilipin‑1 levels, while upregulating adipose triglyceride lipase, phosphorylated (p‑)hormone‑sensitive lipase, p‑adenosine monophosphate activated protein kinase, uncoupling protein 1, peroxisome‑proliferator‑activated receptor‑γ coactivator‑1 α and PR domain containing 16 levels in 3T3‑L1 cells. These findings suggested that AHL may exert immunostimulatory activity through macrophages via TLR4‑mediated activation of p38 and JNK and anti‑obesity activity by blocking lipid accumulation via the inhibition of adipogenesis and induction of lipolysis and browning of white adipocytes.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Childs CE, Calder PC and Miles EA: Diet and immune function. Nutrients. 11(1933)2019.PubMed/NCBI View Article : Google Scholar

2 

Marshall JS, Warrington R, Watson W and Kim HL: An introduction to immunology and immunopathology. Allergy Asthma Clin Immunol. 14 (Suppl 2)(S49)2018.PubMed/NCBI View Article : Google Scholar

3 

Bonilla FA and Oettgen HC: Adaptive immunity. J Allergy Clin Immunol. 125 (2 Suppl 2):S33–S40. 2010.PubMed/NCBI View Article : Google Scholar

4 

Turvey SE and Broide DH: Innate immunity. J Allergy Clin Immunol. 125 (2 Suppl 2):S24–S32. 2010.PubMed/NCBI View Article : Google Scholar

5 

de Frel DL, Atsma DE, Pijl H, Seidell JC, Leenen PJM, Dik WA and van Rossum EFC: The impact of obesity and lifestyle on the immune system and susceptibility to infections such as COVID-19. Front Nutr. 7(597600)2020.PubMed/NCBI View Article : Google Scholar

6 

Muscogiuri G, Pugliese G, Laudisio D, Castellucci B, Barrea L, Savastano S and Colao A: The impact of obesity on immune response to infection: Plausible mechanisms and outcomes. Obes Rev. 22(e13216)2021.PubMed/NCBI View Article : Google Scholar

7 

Martí A, Marcos A and Martínez JA: Obesity and immune function relationships. Obes Rev. 2:131–140. 2001.PubMed/NCBI View Article : Google Scholar

8 

Ahn HS, Kim HJ, Na C, Jang DS, Shin YK and Lee SH: The protective effect of Adenocaulon himalaicum Edgew. and its bioactive compound neochlorogenic acid against UVB-induced skin damage in human dermal fibroblasts and epidermal keratinocytes. Plants (Basel). 10(1669)2021.PubMed/NCBI View Article : Google Scholar

9 

Kwon HC and Lee KR: An acetylene and a monoterpene glycoside from Adenocaulon himalaicum. Planta Med. 67:482–484. 2001.PubMed/NCBI View Article : Google Scholar

10 

Yun JH, Lee SB, Kang K, Lee EH, Lee HJ, Jung SH and Nho CW: Bifunctional chemopreventive effects of Adenocaulon himalaicum through induction of detoxification enzymes and apoptosis. J Med Food. 16:701–710. 2013.PubMed/NCBI View Article : Google Scholar

11 

Teng L, Fu H, Wang M, Deng C and Chen J: Stimulation of RAW264.7 macrophages by sulfated Escherichia coli K5 capsular polysaccharide in vitro. Mol Med Rep. 12:5545–5553. 2015.PubMed/NCBI View Article : Google Scholar

12 

Blaszczak AM, Jalilvand A and Hsueh WA: Adipocytes, innate immunity and obesity: A mini-review. Front Immunol. 12(650768)2021.PubMed/NCBI View Article : Google Scholar

13 

Hirayama D, Iida T and Nakase H: The phagocytic function of macrophage-enforcing innate immunity and tissue homeostasis. Int J Mol Sci. 19(92)2017.PubMed/NCBI View Article : Google Scholar

14 

Mukherjee S, Karmakar S and Babu SPS: TLR2 and TLR4 mediated host immune responses in major infectious diseases: A review. Braz J Infect Dis. 20:193–204. 2016.PubMed/NCBI View Article : Google Scholar

15 

Iwasaki A and Medzhitov R: Toll-like receptor control of the adaptive immune responses. Nat Immunol. 5:987–995. 2004.PubMed/NCBI View Article : Google Scholar

16 

Kawai T and Akira S: Toll-like receptors and their crosstalk with other innate receptors in infection and immunity. Immunity. 34:637–650. 2011.PubMed/NCBI View Article : Google Scholar

17 

Park BS and Lee JO: Recognition of lipopolysaccharide pattern by TLR4 complexes. Exp Mol Med. 45(e66)2013.PubMed/NCBI View Article : Google Scholar

18 

Lee JC, Laydon JT, McDonnell PC, Gallagher TF, Kumar S, Green D, McNulty D, Blumenthal MJ, Heys JR, Landvatter SW, et al: A protein kinase involved in the regulation of inflammatory cytokine biosynthesis. Nature. 372:739–746. 1994.PubMed/NCBI View Article : Google Scholar

19 

Li Y, Meng T, Hao N, Tao H, Zou S, Li M, Ming P, Ding H, Dong J, Feng S, et al: Immune regulation mechanism of Astragaloside IV on RAW264.7 cells through activating the NF-κB/MAPK signaling pathway. Int Immunopharmacol. 49:38–49. 2017.PubMed/NCBI View Article : Google Scholar

20 

Nick JA, Avdi NJ, Gerwins P, Johnson GL and Worthen GS: Activation of a p38 mitogen-activated protein kinase in human neutrophils by lipopolysaccharide. J Immunol. 156:4867–4875. 1996.PubMed/NCBI

21 

Xu Z, Lin R, Hou X, Wu J, Zhao W, Ma H, Fan Z, Li S, Zhu Y and Zhang D: Immunomodulatory mechanism of a purified polysaccharide isolated from Isaria cicadae Miquel on RAW264.7 cells via activating TLR4-MAPK-NF-κB signaling pathway. Int J Biol Macromol. 164:4329–4338. 2020.PubMed/NCBI View Article : Google Scholar

22 

Yang L, Jia X, Fang D, Cheng Y, Zhai Z, Deng W, Du B, Lu T, Wang L, Yang C and Gao Y: Metformin inhibits lipid droplets fusion and growth via reduction in cidec and its regulatory factors in rat adipose-derived stem cells. Int J Mol Sci. 23(5986)2022.PubMed/NCBI View Article : Google Scholar

23 

Guo L, Kang JS, Kang NJ, Je BI, Lee YJ, Park YH and Choi YW: Pelargonidin suppresses adipogenesis in 3T3-L1 cells through inhibition of PPAR-γ signaling pathway. Arch Biochem Biophys. 686(108365)2020.PubMed/NCBI View Article : Google Scholar

24 

Millward CA, Heaney JD, Sinasac DS, Chu EC, Bederman IR, Gilge DA, Previs SF and Croniger CM: Mice with a deletion in the gene for CCAAT/enhancer-binding protein beta are protected against diet-induced obesity. Diabetes. 56:161–167. 2007.PubMed/NCBI View Article : Google Scholar

25 

Langin D: Adipose tissue lipolysis as a metabolic pathway to define pharmacological strategies against obesity and the metabolic syndrome. Pharmacol Res. 53:482–491. 2006.PubMed/NCBI View Article : Google Scholar

26 

Duncan RE, Ahmadian M, Jaworski K, Sarkadi-Nagy E and Sul HS: Regulation of lipolysis in adipocytes. Annu Rev Nutr. 27:79–101. 2007.PubMed/NCBI View Article : Google Scholar

27 

Lass A, Zimmermann RZ, Oberer M and Zechner R: Lipolysis-a highly regulated multi-enzyme complex mediates the catabolism of cellular fat stores. Prog Lipid Res. 50:14–27. 2011.PubMed/NCBI View Article : Google Scholar

28 

Kuryłowicz A and Puzianowska-Kuźnicka M: Induction of adipose tissue browning as a strategy to combat obesity. Int J Mol Sci. 21(6241)2020.PubMed/NCBI View Article : Google Scholar

29 

Lo KA and Sun L: Turning WAT into BAT: A review on regulators controlling the browning of white adipocytes. Biosci Rep. 33(e00065)2013.PubMed/NCBI View Article : Google Scholar

30 

van der Vaart JI, Boon MR and Houtkooper RH: The role of AMPK signaling in brown adipose tissue activation. Cells. 10(1122)2021.PubMed/NCBI View Article : Google Scholar

31 

Cannon B and Nedergaard J: Brown adipose tissue: Function and physiological significance. Physiol Rev. 84:277–359. 2004.PubMed/NCBI View Article : Google Scholar

32 

Seale P, Kajimura S, Yang W, Chin S, Rohas LM, Uldry M, Tavernier G, Langin D and Spiegelman BM: Transcriptional control of brown fat determination by PRDM16. Cell Metab. 6:38–54. 2007.PubMed/NCBI View Article : Google Scholar

33 

Kong L, Xu M, Qiu Y, Liao M, Zhang Q, Yang L and Zheng G: Chlorogenic acid and caffeine combination attenuates adipogenesis by regulating fat metabolism and inhibiting adipocyte differentiation in 3T3-L1 cells. J Food Biochem. 45(e13795)2021.PubMed/NCBI View Article : Google Scholar

34 

Oh JH, Lee JI, Karadeniz F, Seo Y and Kong CS: 3,5-Dicaffeoyl-epi-quinic acid isolated from edible halophyte Atriplex gmelinii inhibits adipogenesis via AMPK/MAPK pathway in 3T3-L1 adipocytes. Evid Based Complement Alternat Med. 2018(8572571)2018.PubMed/NCBI View Article : Google Scholar

35 

Kim JH, Lee S and Cho EJ: Flavonoids from Acer okamotoanum inhibit adipocyte differentiation and promote lipolysis in the 3T3-L1 cells. Molecules. 25(1920)2020.PubMed/NCBI View Article : Google Scholar

36 

Zhou R, Teng L, Zhu Y, Zhang C, Yang Y and Chen Y: Preparation of Amomum longiligulare polysaccharides 1-PLGA nanoparticle and its immune enhancement ability on RAW264.7 cells. Int Immunopharmacol. 99(108053)2021.PubMed/NCBI View Article : Google Scholar

37 

Shen CY, Zhang WL and Jiang JG: Immune-enhancing activity of polysaccharides from Hibiscus sabdariffa Linn. via MAPK and NF-kB signaling pathways in RAW264.7 cells. J Funct Foods. 34:118–129. 2017.

38 

Lin S, Li HY, Wang ZY, Liu X, Yang Y, Cao ZW, Du G, Zhao L, Zhang Q, Wu DT and Qin W: Analysis of methanolic extracts and crude polysaccharides from the leaves of Chuanminshen violaceum and their antioxidant activities. Antioxidants (Basel). 8(266)2019.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Choi HJ, Choi JW, Im SW and Jeong JB: Immunostimulatory and anti‑obesity activities of <em>Adenocaulon himalaicum</em> leaves in RAW264.7 and 3T3‑L1 cells. Exp Ther Med 25: 219, 2023.
APA
Choi, H.J., Choi, J.W., Im, S.W., & Jeong, J.B. (2023). Immunostimulatory and anti‑obesity activities of <em>Adenocaulon himalaicum</em> leaves in RAW264.7 and 3T3‑L1 cells. Experimental and Therapeutic Medicine, 25, 219. https://doi.org/10.3892/etm.2023.11918
MLA
Choi, H. J., Choi, J. W., Im, S. W., Jeong, J. B."Immunostimulatory and anti‑obesity activities of <em>Adenocaulon himalaicum</em> leaves in RAW264.7 and 3T3‑L1 cells". Experimental and Therapeutic Medicine 25.5 (2023): 219.
Chicago
Choi, H. J., Choi, J. W., Im, S. W., Jeong, J. B."Immunostimulatory and anti‑obesity activities of <em>Adenocaulon himalaicum</em> leaves in RAW264.7 and 3T3‑L1 cells". Experimental and Therapeutic Medicine 25, no. 5 (2023): 219. https://doi.org/10.3892/etm.2023.11918
Copy and paste a formatted citation
x
Spandidos Publications style
Choi HJ, Choi JW, Im SW and Jeong JB: Immunostimulatory and anti‑obesity activities of <em>Adenocaulon himalaicum</em> leaves in RAW264.7 and 3T3‑L1 cells. Exp Ther Med 25: 219, 2023.
APA
Choi, H.J., Choi, J.W., Im, S.W., & Jeong, J.B. (2023). Immunostimulatory and anti‑obesity activities of <em>Adenocaulon himalaicum</em> leaves in RAW264.7 and 3T3‑L1 cells. Experimental and Therapeutic Medicine, 25, 219. https://doi.org/10.3892/etm.2023.11918
MLA
Choi, H. J., Choi, J. W., Im, S. W., Jeong, J. B."Immunostimulatory and anti‑obesity activities of <em>Adenocaulon himalaicum</em> leaves in RAW264.7 and 3T3‑L1 cells". Experimental and Therapeutic Medicine 25.5 (2023): 219.
Chicago
Choi, H. J., Choi, J. W., Im, S. W., Jeong, J. B."Immunostimulatory and anti‑obesity activities of <em>Adenocaulon himalaicum</em> leaves in RAW264.7 and 3T3‑L1 cells". Experimental and Therapeutic Medicine 25, no. 5 (2023): 219. https://doi.org/10.3892/etm.2023.11918
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team