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The hepatoprotective effect of aspirin on carbon tetrachloride‑induced hepatic fibrosis via inhibition of TGFβ‑1 pathway and pro‑inflammatory cytokines IL‑1β and COX‑2 in rats

  • Authors:
    • Zhiguo Wu
    • Yuchan Wu
    • Weicheng Zhong
    • Qingmei Zhong
    • Shengfang Rao
    • Dongshan Yu
    • Xiaolu Luo
    • Fang Qiu
    • Zhiying Song
    • Di Jin
    • Maoxing Ai
    • Yu Lan
    • Gongchang Zhang
    • Shufeng Song
    • Baogang Xie
    • Shuilin Sun
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Diseases, The Second Affiliated Hospital of Nanchang University, Nanchang University Hospital, Nanchang, Jiangxi 330006, P.R. China, Department of Pathology, The Ninth Hospital of Nanchang, Nanchang, Jiangxi 330006, P.R. China, Department of Nuclear Medicine, Nanchang University Hospital, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 232
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    Published online on: March 31, 2023
       https://doi.org/10.3892/etm.2023.11931
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Abstract

Aspirin decreases liver fibrosis index and inflammation levels. However, the exact mechanism underlying the effects of aspirin are yet to be elucidated. The aim of the study was to investigate the potential protective effects of aspirin on carbon tetrachloride (CCl4)‑induced hepatic fibrosis in Sprague‑Dawley rats. Rats were divided into four groups, including healthy and CCl4 control and low‑(aspirin 10 mg/kg + CCl4) and high‑dose aspirin group (aspirin 300 mg/kg + CCl4). After 8 weeks treatment, the histopathological examinations of hepatocyte fibrosis in liver and serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), IL‑1β, transforming growth factor‑β1 (TGF‑β1), hyaluronic acid (HA), laminin (LN) and type IV collagen (IV.C) were determined. Histopathological examination suggested that aspirin decreased CCl4‑induced hepatic fibrosis and liver inflammation. The high‑dose aspirin group significantly decreased the serum levels of ALT, AST, HA and LN compared with the CCl4 control group. High‑dose aspirin group significantly decreased the levels of pro‑inflammatory cytokines IL‑1β compared with CCl4 group. The high‑dose aspirin group significantly inhibited the expression of TGFβ‑1 protein compared with CCl4 group. Overall, the present study indicated that aspirin exhibited potent protective effects against CCl4‑induced hepatic fibrosis via inhibition of the TGFβ‑1 pathway and pro‑inflammatory cytokine IL‑1β.
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Copy and paste a formatted citation
Spandidos Publications style
Wu Z, Wu Y, Zhong W, Zhong Q, Rao S, Yu D, Luo X, Qiu F, Song Z, Jin D, Jin D, et al: The hepatoprotective effect of aspirin on carbon tetrachloride‑induced hepatic fibrosis via inhibition of TGFβ‑1 pathway and pro‑inflammatory cytokines IL‑1β and COX‑2 in rats. Exp Ther Med 25: 232, 2023.
APA
Wu, Z., Wu, Y., Zhong, W., Zhong, Q., Rao, S., Yu, D. ... Sun, S. (2023). The hepatoprotective effect of aspirin on carbon tetrachloride‑induced hepatic fibrosis via inhibition of TGFβ‑1 pathway and pro‑inflammatory cytokines IL‑1β and COX‑2 in rats. Experimental and Therapeutic Medicine, 25, 232. https://doi.org/10.3892/etm.2023.11931
MLA
Wu, Z., Wu, Y., Zhong, W., Zhong, Q., Rao, S., Yu, D., Luo, X., Qiu, F., Song, Z., Jin, D., Ai, M., Lan, Y., Zhang, G., Song, S., Xie, B., Sun, S."The hepatoprotective effect of aspirin on carbon tetrachloride‑induced hepatic fibrosis via inhibition of TGFβ‑1 pathway and pro‑inflammatory cytokines IL‑1β and COX‑2 in rats". Experimental and Therapeutic Medicine 25.5 (2023): 232.
Chicago
Wu, Z., Wu, Y., Zhong, W., Zhong, Q., Rao, S., Yu, D., Luo, X., Qiu, F., Song, Z., Jin, D., Ai, M., Lan, Y., Zhang, G., Song, S., Xie, B., Sun, S."The hepatoprotective effect of aspirin on carbon tetrachloride‑induced hepatic fibrosis via inhibition of TGFβ‑1 pathway and pro‑inflammatory cytokines IL‑1β and COX‑2 in rats". Experimental and Therapeutic Medicine 25, no. 5 (2023): 232. https://doi.org/10.3892/etm.2023.11931
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Z, Wu Y, Zhong W, Zhong Q, Rao S, Yu D, Luo X, Qiu F, Song Z, Jin D, Jin D, et al: The hepatoprotective effect of aspirin on carbon tetrachloride‑induced hepatic fibrosis via inhibition of TGFβ‑1 pathway and pro‑inflammatory cytokines IL‑1β and COX‑2 in rats. Exp Ther Med 25: 232, 2023.
APA
Wu, Z., Wu, Y., Zhong, W., Zhong, Q., Rao, S., Yu, D. ... Sun, S. (2023). The hepatoprotective effect of aspirin on carbon tetrachloride‑induced hepatic fibrosis via inhibition of TGFβ‑1 pathway and pro‑inflammatory cytokines IL‑1β and COX‑2 in rats. Experimental and Therapeutic Medicine, 25, 232. https://doi.org/10.3892/etm.2023.11931
MLA
Wu, Z., Wu, Y., Zhong, W., Zhong, Q., Rao, S., Yu, D., Luo, X., Qiu, F., Song, Z., Jin, D., Ai, M., Lan, Y., Zhang, G., Song, S., Xie, B., Sun, S."The hepatoprotective effect of aspirin on carbon tetrachloride‑induced hepatic fibrosis via inhibition of TGFβ‑1 pathway and pro‑inflammatory cytokines IL‑1β and COX‑2 in rats". Experimental and Therapeutic Medicine 25.5 (2023): 232.
Chicago
Wu, Z., Wu, Y., Zhong, W., Zhong, Q., Rao, S., Yu, D., Luo, X., Qiu, F., Song, Z., Jin, D., Ai, M., Lan, Y., Zhang, G., Song, S., Xie, B., Sun, S."The hepatoprotective effect of aspirin on carbon tetrachloride‑induced hepatic fibrosis via inhibition of TGFβ‑1 pathway and pro‑inflammatory cytokines IL‑1β and COX‑2 in rats". Experimental and Therapeutic Medicine 25, no. 5 (2023): 232. https://doi.org/10.3892/etm.2023.11931
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