Tyrosol attenuates lipopolysaccharide‑induced inflammation in HUVECs to promote vascular health against atherosclerosis challenge

Zhao,Weiwei; Wei,Huizhen; Lu,Jun; Sha,Wenjun; Sun,Dusang; Pan,Ting; Lei,Tao

doi:10.3892/etm.2023.11939

Tyrosol attenuates lipopolysaccharide‑induced inflammation in HUVECs to promote vascular health against atherosclerosis challenge

Authors:
- Weiwei Zhao
- Huizhen Wei
- Jun Lu
- Wenjun Sha
- Dusang Sun
- Ting Pan
- Tao Lei
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Affiliations: Department of Endocrinology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China, School of Medical and Life Sciences, Reproductive and Women‑Children Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu 610041, P.R. China
Published online on: April 6, 2023 https://doi.org/10.3892/etm.2023.11939
Article Number: 240
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The role of vascular endothelial cells in acute and chronic vascular inflammatory response has long been recognized. Therefore, persistent vascular inflammation may lead to endothelial dysfunction, thus resulting in the release of pro‑inflammatory cytokines and the expression of adhesion molecules, which in turn promote monocyte/macrophage adhesion. Inflammation serves a key role in the development of vascular diseases, such as atherosclerosis. Tyrosol is a natural polyphenolic compound with diverse biological functions, found in large quantities in olive oil or in Rhodiola rosea. The current study aimed to investigate the regulatory in vitro effects of tyrosol on pro‑inflammatory phenotypes using Cell Counting Kit‑8, cell adhesion assay, wound healing, ELISA, western blotting, duel‑luciferase, reverse transcription‑quantitative PCR and flow cytometry. The results showed that tyrosol significantly inhibited the adhesion of THP‑1 human umbilical vein endothelial cells, reduced lipopolysaccharide‑induced cell migration and decreased the release of pro‑inflammatory factors and the expression levels of adhesion‑related molecules, such as TNF‑α, monocyte chemotactic protein‑1, intercellular adhesion molecule‑1 and vascular cell adhesion molecule‑1. Previous studies indicate that NF‑κB could serve a pivotal role in initiating the inflammatory responses of endothelial cells and particularly in regulating the expression of adhesion molecules and inflammatory factors. The results of the current study demonstrated that tyrosol was associated with decreased expression of adhesion molecules and monocyte‑endothelial cell adhesion, thus suggesting that tyrosol could be a novel pharmacological approach for treating inflammatory vascular diseases.

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