Open Access

Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery

  • Authors:
    • Sijia Yu
    • Xiaoli Dong
    • Wenguang Lai
    • Hongyu Lu
    • Yun Xie
    • Jun-Yan Xu
    • Yewen Zeng
    • Kedong Han
    • Jinqiang Liang
    • Jin Liu
    • Yong Liu
    • Jiyan Chen
  • View Affiliations

  • Published online on: May 15, 2023     https://doi.org/10.3892/etm.2023.12020
  • Article Number: 321
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Acute kidney injury (AKI) is a common complication after acute myocardial infarction (AMI) in clinical practice, and the majority of previous preclinical models were induced by a single factor. The objective of the present study was to establish a stable preclinic model of AKI induced by contrast media (CM) with acute myocardial ischemia reperfusion surgery and to identify the effect of oxidative stress on kidney injury. Rats were treated individually or with CM or myocardial ischemia reperfusion surgery. Renal baseline and AKI parameters, the level of oxidative stress and histopathological images were examined along with AKI biomarkers. Results showed the incidence of AKI in the CM group and ischemia reperfusion injury (IRI) group was 40%, χ2 test (P<0.05 vs. CM‑IRI) and 35%, χ2 test (P<0.05 vs. CM‑IRI) and the combination group had the highest incidence rate 75%. IRI surgery combined with CM diminished kidney function and induced oxidative stress by increasing creatinine, blood urea nitrogen and reactive oxygen species levels. Western blotting showed that the early AKI biomarker of NGAL and KIM‑1 increased and that the combination group had the highest value. Pathology damage exhibited severe kidney damage in the combination group compared with other control groups. The present research established a reliable preclinic model of post‑AMI AKI with a stable and high postoperative AKI rate. Additionally, CM was demonstrated to exacerbate AKI caused by acute myocardial infarction through oxidative stress and, thus, oxidative stress may be a potential therapeutic target.
View Figures
View References

Related Articles

Journal Cover

July-2023
Volume 26 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yu S, Dong X, Lai W, Lu H, Xie Y, Xu J, Zeng Y, Han K, Liang J, Liu J, Liu J, et al: Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery. Exp Ther Med 26: 321, 2023
APA
Yu, S., Dong, X., Lai, W., Lu, H., Xie, Y., Xu, J. ... Chen, J. (2023). Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery. Experimental and Therapeutic Medicine, 26, 321. https://doi.org/10.3892/etm.2023.12020
MLA
Yu, S., Dong, X., Lai, W., Lu, H., Xie, Y., Xu, J., Zeng, Y., Han, K., Liang, J., Liu, J., Liu, Y., Chen, J."Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery". Experimental and Therapeutic Medicine 26.1 (2023): 321.
Chicago
Yu, S., Dong, X., Lai, W., Lu, H., Xie, Y., Xu, J., Zeng, Y., Han, K., Liang, J., Liu, J., Liu, Y., Chen, J."Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery". Experimental and Therapeutic Medicine 26, no. 1 (2023): 321. https://doi.org/10.3892/etm.2023.12020