<em>Diospyros lotus</em> leaf extract and its main component myricitrin regulate pruritus through the inhibition of astrocyte activation

Shin,Jae Young; Cho,Byoung Ok; Park,Ji Hyeon; Kang,Eun Seo; Kim,Young Soo; Jang,Seon Il

doi:10.3892/etm.2023.12022

Diospyros lotus leaf extract and its main component myricitrin regulate pruritus through the inhibition of astrocyte activation

Authors:
- Jae Young Shin
- Byoung Ok Cho
- Ji Hyeon Park
- Eun Seo Kang
- Young Soo Kim
- Seon Il Jang
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Affiliations: Department of Food Science and Technology, Jeonbuk National University, Deokjin, Jeonju, Jeollabuk 54896, Republic of Korea, Institute of Health and Science, Jeonju University, Wansan, Jeonju, Jeollabuk 55069, Republic of Korea
Published online on: May 16, 2023 https://doi.org/10.3892/etm.2023.12022
Article Number: 323
Copyright: © Shin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diospyros lotus is a deciduous plant native to Asian countries, including Korea, Japan and China, and southeast Europe. In traditional medicine, Diospyros lotus is used as an anticancer, antidiabetic and antipyretic agent. The present study aimed to evaluate the effect of Diospyros lotus leaf extract (DLE) in ameliorating histamine‑independent pruritus. Activation of signal transducer and activator of transcription 3 (STAT3) in astrocytes contributes to pruritus. In this study, the effects of DLE and its main component, myricetin (MC), on the activation of STAT3, expression of glial fibrillary acidic protein (GFAP), and production of lipocalin‑2 (LCN2) in IL‑6‑treated astrocytes and chloroquine‑injected mice were investigated through western blot, reverse transcription‑quantitative PCR, and immunofluorescence staining. DLE and MC inhibited STAT3 activation, GFAP expression and LCN2 release via inositol 1,4,5‑trisphosphate receptor type 1 blockade in astrocytes. DLE and MC ameliorated scratching behavior, expression of GFAP, mast cell infiltration and serum IL‑6 levels in chloroquine‑injected mice. These results suggested that DLE and MC can be used as oral therapeutic agents for the treatment and management of pruritus.

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