Protective effects of hsa_circ_0072568 on interleukin‑1β‑stimulated human chondrocytes are mediated via the miR‑382‑5p/TOP1 axis

Chang,Qing; Li,Chao; Hu,Junzheng; Geng,Rui

doi:10.3892/etm.2023.12082

Protective effects of hsa_circ_0072568 on interleukin‑1β‑stimulated human chondrocytes are mediated via the miR‑382‑5p/TOP1 axis

Authors:
- Qing Chang
- Chao Li
- Junzheng Hu
- Rui Geng
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Affiliations: Department of Orthopaedic Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu 210009, P.R. China
Published online on: June 28, 2023 https://doi.org/10.3892/etm.2023.12082
Article Number: 383
Copyright: © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNA (circRNA) dysregulation has been linked to osteoarthritis (OA). The present study investigated the involvement of hsa_circ_0072568 (circ0072568) in OA. The expression of circ0072568 was detected in OA tissues and interleukin (IL)‑1β‑stimulated human chondrocytes. After performing dual‑luciferase reporter and RNA immunoprecipitation assays, MTT, enzyme‑linked immunosorbent assay and western blot analysis were used to assess the functions of circ0072568 in IL‑1β‑induced inflammation in chondrocytes in vitro. Circ0072568 was inhibited in OA tissues and the cell model in vitro. Circ0072568 overexpression protected the chondrocytes against IL‑1β‑induced inflammation and extracellular matrix (ECM) breakdown. Circ0072568 directly attached to microRNA (miR)‑382‑5p and enhanced the production of topoisomerase 1 (TOP1). Furthermore, miR‑382‑5p overexpression or TOP1 knockdown attenuated the effects of circ0072568 in IL‑1β‑stimulated human chondrocytes. On the whole, the present study demonstrates that the Circ0072568/miR‑382‑5p/TOP1 axis is involved in inflammation and ECM degradation in OA. These findings may contribute to the development of potential therapeutic strategies for OA.

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