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Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression

  • Authors:
    • Yujie Xu
    • Dingmao Wang
    • Guodong Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Haikou People's Hospital, Haikou, Hainan 570208, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 392
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    Published online on: July 3, 2023
       https://doi.org/10.3892/etm.2023.12091
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Abstract

Colon cancer is a highly invasive and metastatic cancer with a poor prognosis. The University of Alabama at Birmingham Cancer data analysis portal (UALCAN) database indicates that potassium voltage‑gated channel subfamily E member 4 (KCNE4) is highly expressed in colon cancer tissues. UALCAN data also show that KCNE4 expression is positively associated with individual cancer stages and negatively associated with patient survival. Therefore, the aim of the current study was to elucidate the functional role of KCNE4 in the biological behaviors of colon cancer cells and to investigate the underlying molecular mechanism. The gene EGF containing fibulin extracellular matrix protein 2 (EFEMP2) was found to be positively correlated with KCNE4 in colon cancer based on analysis performed using the LinkedOmics database; notably, upregulated EFEMP2 expression has been reported to be closely associated with the malignant phenotypes of colon cancer cells. The differences in the expression levels of KCNE4 and EFEMP2 between human colon cancer and normal colonic mucosa cell lines were assessed via reverse transcription‑quantitative PCR and western blot assays. In addition, the proliferation, migration and invasion of colon cancer cells were determined using Cell Counting kit‑8, colony formation, would healing and Transwell assays, and a co‑immunoprecipitation assay was performed to confirm the interaction between KCNE4 and EFEMP2. The results of the study demonstrated that KCNE4 and EFEMP2 are markedly upregulated in colon cancer cells. In addition, KCNE4 interacted with and bound to EFEMP2. The suppressive effects of KCNE4 knockdown on the proliferation, colony formation, migration and invasion of colon cancer cells were attenuated by EFEMP2 overexpression. On the basis of these findings, it may be concluded that KCNE4 acts as an oncogene in colon cancer via the promotion of EFEMP2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Wang D and Zhao G: Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression. Exp Ther Med 26: 392, 2023.
APA
Xu, Y., Wang, D., & Zhao, G. (2023). Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression. Experimental and Therapeutic Medicine, 26, 392. https://doi.org/10.3892/etm.2023.12091
MLA
Xu, Y., Wang, D., Zhao, G."Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression". Experimental and Therapeutic Medicine 26.2 (2023): 392.
Chicago
Xu, Y., Wang, D., Zhao, G."Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression". Experimental and Therapeutic Medicine 26, no. 2 (2023): 392. https://doi.org/10.3892/etm.2023.12091
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Wang D and Zhao G: Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression. Exp Ther Med 26: 392, 2023.
APA
Xu, Y., Wang, D., & Zhao, G. (2023). Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression. Experimental and Therapeutic Medicine, 26, 392. https://doi.org/10.3892/etm.2023.12091
MLA
Xu, Y., Wang, D., Zhao, G."Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression". Experimental and Therapeutic Medicine 26.2 (2023): 392.
Chicago
Xu, Y., Wang, D., Zhao, G."Potassium voltage‑gated channel subfamily E member 4 facilitates the malignant progression of colon cancer by enhancing EGF containing fibulin extracellular matrix protein 2 expression". Experimental and Therapeutic Medicine 26, no. 2 (2023): 392. https://doi.org/10.3892/etm.2023.12091
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