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Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling

  • Authors:
    • Jiang Li
    • Hong Huang
    • Shanhu Xu
    • Mengge Fan
    • Kaili Wang
    • Xia Wang
    • Jiao Zhang
    • Shengshi Huang
    • Alex Gatt
    • Ju Liu
  • View Affiliations / Copyright

    Affiliations: Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Institute of Microvascular Medicine, Medical Research Center, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, P.R. China, Department of Infectious Diseases, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China, Department of Pathology, Faculty of Medicine and Surgery, University of Malta, Msida, MSD 2080, Malta
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 408
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    Published online on: July 10, 2023
       https://doi.org/10.3892/etm.2023.12107
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Abstract

Complement factor H (CFH), a major soluble inhibitor of complement, is a plasma protein that directly interacts with the endothelium of blood vessels. Mutations in the CFH gene lead to diseases associated with excessive angiogenesis; however, the underlying mechanisms are unknown. The present study aimed to determine the effects of CFH on endothelial cells and to explore the underlying mechanisms. The adenoviral plasmid expressing CFH was transduced into HepG2 cells, and the culture medium supernatant was collected and co‑cultured with human umbilical vein endothelial cells (HUVECs). Cell proliferation was measured by CCK8 and MTT assays, and cell migration was measured by wound healing and Transwell assays. Reverse transcription‑quantitative PCR was performed to detect gene transcription. Western blotting was used to determine protein levels. The results revealed that CFH can inhibit migration, but not viability, of HUVECs. In addition, CFH did not significantly alter MAPK or TGF‑β signaling, whereas it decreased STAT3 phosphorylation in HUVECs. Furthermore, CFH failed to reduce migration of HUVECs, with inhibition of STAT3 signaling by STATTIC or activation of STAT3 signaling by overexpression of STAT3 (Y705D) compromising CFH‑inhibited HUVEC migration. CFH also decreased the expression levels of vascular endothelial growth factor receptor 2, a downstream effector of STAT3 mediating endothelial cell migration. In conclusion, the present study suggested that CFH may be a potential therapeutic target for angiogenesis‑related diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Huang H, Xu S, Fan M, Wang K, Wang X, Zhang J, Huang S, Gatt A, Liu J, Liu J, et al: Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling. Exp Ther Med 26: 408, 2023.
APA
Li, J., Huang, H., Xu, S., Fan, M., Wang, K., Wang, X. ... Liu, J. (2023). Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling. Experimental and Therapeutic Medicine, 26, 408. https://doi.org/10.3892/etm.2023.12107
MLA
Li, J., Huang, H., Xu, S., Fan, M., Wang, K., Wang, X., Zhang, J., Huang, S., Gatt, A., Liu, J."Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling". Experimental and Therapeutic Medicine 26.2 (2023): 408.
Chicago
Li, J., Huang, H., Xu, S., Fan, M., Wang, K., Wang, X., Zhang, J., Huang, S., Gatt, A., Liu, J."Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling". Experimental and Therapeutic Medicine 26, no. 2 (2023): 408. https://doi.org/10.3892/etm.2023.12107
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Huang H, Xu S, Fan M, Wang K, Wang X, Zhang J, Huang S, Gatt A, Liu J, Liu J, et al: Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling. Exp Ther Med 26: 408, 2023.
APA
Li, J., Huang, H., Xu, S., Fan, M., Wang, K., Wang, X. ... Liu, J. (2023). Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling. Experimental and Therapeutic Medicine, 26, 408. https://doi.org/10.3892/etm.2023.12107
MLA
Li, J., Huang, H., Xu, S., Fan, M., Wang, K., Wang, X., Zhang, J., Huang, S., Gatt, A., Liu, J."Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling". Experimental and Therapeutic Medicine 26.2 (2023): 408.
Chicago
Li, J., Huang, H., Xu, S., Fan, M., Wang, K., Wang, X., Zhang, J., Huang, S., Gatt, A., Liu, J."Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling". Experimental and Therapeutic Medicine 26, no. 2 (2023): 408. https://doi.org/10.3892/etm.2023.12107
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