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Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response

  • Authors:
    • Xi Huang
    • Qi Ji
    • Chen-Yi She
    • Yi Cheng
    • Jian-Rong Zhou
    • Qing-Ming Wu
  • View Affiliations / Copyright

    Affiliations: Medical College, Wuhan University of Science and Technology, Wuhan, Hubei 430065, P.R. China, Department of Gastroenterology, General Hospital of Central Theater Command, Wuhan, Hubei 430064, P.R. China, Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510000, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 418
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    Published online on: July 13, 2023
       https://doi.org/10.3892/etm.2023.12116
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Abstract

Lipid metabolic disorders, oxidative stress and inflammation in the liver are key steps in the progression of non‑alcoholic fatty liver disease (NAFLD). Ophiopogonin D (OP‑D), the main active ingredient of Ophiopogon japonicus, exhibits several pharmacological activities such as antioxidant and anti‑inflammatory activities. Therefore, the current study aimed to explore the role of OP‑D in NAFLD in a high‑fat diet (HFD)‑induced obesity mouse model. To investigate the effect of OP‑D on NAFLD in vivo, a NAFLD mouse model was established following feeding mice with HFD, then the mice were randomly treated with HFD or HFD + OP‑D for 4 weeks. Subsequently, primary mouse hepatocytes were isolated, and enzyme‑linked immunosorbent assay, reverse transcription‑quantitative PCR western blotting and immunofluorescence analysis were used for assessment to explore the direct effect of OP‑D in vitro. The results of the present study indicated that OP‑D could ameliorate NAFLD in HFD‑induced obese mice by regulating lipid metabolism and antioxidant and anti‑inflammatory responses. Additionally, OP‑D treatment decreased lipogenesis and inflammation levels in vitro, suggesting that the NF‑κB signaling pathway may be involved in the beneficial effects of OP‑D on NAFLD.
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Copy and paste a formatted citation
Spandidos Publications style
Huang X, Ji Q, She C, Cheng Y, Zhou J and Wu Q: Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response. Exp Ther Med 26: 418, 2023.
APA
Huang, X., Ji, Q., She, C., Cheng, Y., Zhou, J., & Wu, Q. (2023). Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response. Experimental and Therapeutic Medicine, 26, 418. https://doi.org/10.3892/etm.2023.12116
MLA
Huang, X., Ji, Q., She, C., Cheng, Y., Zhou, J., Wu, Q."Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response". Experimental and Therapeutic Medicine 26.3 (2023): 418.
Chicago
Huang, X., Ji, Q., She, C., Cheng, Y., Zhou, J., Wu, Q."Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response". Experimental and Therapeutic Medicine 26, no. 3 (2023): 418. https://doi.org/10.3892/etm.2023.12116
Copy and paste a formatted citation
x
Spandidos Publications style
Huang X, Ji Q, She C, Cheng Y, Zhou J and Wu Q: Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response. Exp Ther Med 26: 418, 2023.
APA
Huang, X., Ji, Q., She, C., Cheng, Y., Zhou, J., & Wu, Q. (2023). Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response. Experimental and Therapeutic Medicine, 26, 418. https://doi.org/10.3892/etm.2023.12116
MLA
Huang, X., Ji, Q., She, C., Cheng, Y., Zhou, J., Wu, Q."Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response". Experimental and Therapeutic Medicine 26.3 (2023): 418.
Chicago
Huang, X., Ji, Q., She, C., Cheng, Y., Zhou, J., Wu, Q."Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response". Experimental and Therapeutic Medicine 26, no. 3 (2023): 418. https://doi.org/10.3892/etm.2023.12116
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