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Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells

  • Authors:
    • Yong Liu
    • Wei Wang
    • Yong Zeng
    • Hui Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Chengdu Second People's Hospital, Chengdu, Sichuan 610017, P.R. China, Department of Human Anatomy and Histoembryology, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong 519041, P.R. China, Department of Bone and Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 436
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    Published online on: July 31, 2023
       https://doi.org/10.3892/etm.2023.12135
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Abstract

Hydrogen (H2) is a major biodegradation product of implanted magnesium (Mg) alloys that are commonly used in the healing of bone fractures. Our earlier study showed that H2 can inhibit mouse bone marrow mononuclear cell (BMMC) osteoclastogenesis during the differentiation of these cells into osteoclasts, thereby facilitating fracture healing. However, the way by which H2 inhibits osteoclastogenesis remains to be elucidated. The present study used RNA‑sequencing to study the transcriptome of H2‑exposed BMMCs in an osteoclast‑induced environment and identified the target genes and signaling pathways through which H2 exerts its biological effects. Several upregulated genes were identified: Fos, Dusp1, Cxcl1, Reln, Itga2b, Plin2, Lif, Thbs1, Vegfa and Gadd45a. Several downregulated genes were also revealed: Hspa1b, Gm4951, F830016B08Rik, Fads2, Hspa1a, Slc27a6, Cacna1b, Scd2, Lama3 and Col4a5. These differentially expressed genes were mainly involved in osteoclast differentiation cascades, as well as PI3K‑AKT, Forkhead box O (FoxO), MAPK, peroxisome proliferator‑activated receptor (PPAR), TNF, TGF‑β, JAK‑STAT, RAS, VEGF, hypoxia‑inducible factor (HIF‑1) and AMPK signaling pathways. In summary, the present study revealed the key genes and signaling pathways involved in the H2‑mediated inhibition of osteoclastogenesis, thereby providing a theoretical basis for the significance of H2 and an experimental basis for the application of Mg alloys in the treatment of osteoporosis.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Wang W, Zeng Y and Zeng H: Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells. Exp Ther Med 26: 436, 2023.
APA
Liu, Y., Wang, W., Zeng, Y., & Zeng, H. (2023). Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells. Experimental and Therapeutic Medicine, 26, 436. https://doi.org/10.3892/etm.2023.12135
MLA
Liu, Y., Wang, W., Zeng, Y., Zeng, H."Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells". Experimental and Therapeutic Medicine 26.3 (2023): 436.
Chicago
Liu, Y., Wang, W., Zeng, Y., Zeng, H."Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells". Experimental and Therapeutic Medicine 26, no. 3 (2023): 436. https://doi.org/10.3892/etm.2023.12135
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Wang W, Zeng Y and Zeng H: Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells. Exp Ther Med 26: 436, 2023.
APA
Liu, Y., Wang, W., Zeng, Y., & Zeng, H. (2023). Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells. Experimental and Therapeutic Medicine, 26, 436. https://doi.org/10.3892/etm.2023.12135
MLA
Liu, Y., Wang, W., Zeng, Y., Zeng, H."Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells". Experimental and Therapeutic Medicine 26.3 (2023): 436.
Chicago
Liu, Y., Wang, W., Zeng, Y., Zeng, H."Transcriptome analysis of hydrogen inhibits osteoclastogenesis of mouse bone marrow mononuclear cells". Experimental and Therapeutic Medicine 26, no. 3 (2023): 436. https://doi.org/10.3892/etm.2023.12135
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