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Relief of gouty arthritis in rats by total saponins from Dioscorea nipponica Makino through suppression of neutrophil extracellular trap formation via the PI3K/AKT/mTOR axis

  • Authors:
    • Qi Zhou
    • Lin Liu
    • Huijuan Sun
    • Shumin Liu
  • View Affiliations / Copyright

    Affiliations: Research Institute of Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China, Graduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China, Technological Innovation Team of Basic Theory Study Research of Institution of Higher Education in Heilongjiang, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 447
    |
    Published online on: August 2, 2023
       https://doi.org/10.3892/etm.2023.12146
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Abstract

Gouty arthritis (GA) is an inflammatory disorder that is associated with elevated serum levels of uric acid. Total saponins from Dioscorea nipponica Makino (TSDN) are a natural component that ameliorates inflammation while also decreasing uric acid levels. The aim of the present study was to unravel the mechanism of TSDN in gouty rats in regard to regulation of the formation of neutrophil extracellular traps (NETs) via the PI3K/AKT/mTOR axis. A total of 40 Wistar rats were divided into 4 groups: normal, model, TSDN and rapamycin groups. Reverse‑transcription‑quantitative PCR (RT‑qPCR) and western blot analysis were used to assess the mRNA and protein expression levels of the PI3K/AKT/mTOR axis. The formation of NETs was detected by immunohistochemical and immunofluorescent methods. ELISA was used to measure the levels of IL‑1β and TNF‑α. RT‑qPCR and western blotting demonstrated that TSDN compromised the mRNA and protein expression levels of activated protein kinase (AMPK) and mTOR, as well as the mRNA expression levels of AKT and PTEN. Furthermore, it increased the protein expression levels of phosphorylated (p‑) PI3K, p‑AKT and p‑AMPK. Immunohistochemical and immunofluorescent analyses revealed that TSDN decreased the protein expression levels of neutrophil elastase, proteinase 3, cathepsin G, lactoferrin and myeloperoxidase, as well as the number of citrullinated histone 3+ cells. TSDN also reduced the release of IL‑1β and TNF‑α. Overall, the anti‑inflammatory action of TSDN in gouty rats may be realized by suppressing the formation of NETs by regulating the PI3K/AKT/mTOR axis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Q, Liu L, Sun H and Liu S: Relief of gouty arthritis in rats by total saponins from<em> Dioscorea nipponica</em> Makino through suppression of neutrophil extracellular trap formation via the PI3K/AKT/mTOR axis. Exp Ther Med 26: 447, 2023.
APA
Zhou, Q., Liu, L., Sun, H., & Liu, S. (2023). Relief of gouty arthritis in rats by total saponins from<em> Dioscorea nipponica</em> Makino through suppression of neutrophil extracellular trap formation via the PI3K/AKT/mTOR axis. Experimental and Therapeutic Medicine, 26, 447. https://doi.org/10.3892/etm.2023.12146
MLA
Zhou, Q., Liu, L., Sun, H., Liu, S."Relief of gouty arthritis in rats by total saponins from<em> Dioscorea nipponica</em> Makino through suppression of neutrophil extracellular trap formation via the PI3K/AKT/mTOR axis". Experimental and Therapeutic Medicine 26.3 (2023): 447.
Chicago
Zhou, Q., Liu, L., Sun, H., Liu, S."Relief of gouty arthritis in rats by total saponins from<em> Dioscorea nipponica</em> Makino through suppression of neutrophil extracellular trap formation via the PI3K/AKT/mTOR axis". Experimental and Therapeutic Medicine 26, no. 3 (2023): 447. https://doi.org/10.3892/etm.2023.12146
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Q, Liu L, Sun H and Liu S: Relief of gouty arthritis in rats by total saponins from<em> Dioscorea nipponica</em> Makino through suppression of neutrophil extracellular trap formation via the PI3K/AKT/mTOR axis. Exp Ther Med 26: 447, 2023.
APA
Zhou, Q., Liu, L., Sun, H., & Liu, S. (2023). Relief of gouty arthritis in rats by total saponins from<em> Dioscorea nipponica</em> Makino through suppression of neutrophil extracellular trap formation via the PI3K/AKT/mTOR axis. Experimental and Therapeutic Medicine, 26, 447. https://doi.org/10.3892/etm.2023.12146
MLA
Zhou, Q., Liu, L., Sun, H., Liu, S."Relief of gouty arthritis in rats by total saponins from<em> Dioscorea nipponica</em> Makino through suppression of neutrophil extracellular trap formation via the PI3K/AKT/mTOR axis". Experimental and Therapeutic Medicine 26.3 (2023): 447.
Chicago
Zhou, Q., Liu, L., Sun, H., Liu, S."Relief of gouty arthritis in rats by total saponins from<em> Dioscorea nipponica</em> Makino through suppression of neutrophil extracellular trap formation via the PI3K/AKT/mTOR axis". Experimental and Therapeutic Medicine 26, no. 3 (2023): 447. https://doi.org/10.3892/etm.2023.12146
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