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Effects of deferoxamine on intrinsic colistin resistance of Proteus mirabilis

  • Authors:
    • Mehmet Erinmez
    • Yasemin Zer
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    Affiliations: Department of Medical Microbiology, Gaziantep University School of Medicine, 27310 Gaziantep, Turkey
    Copyright: © Erinmez et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 459
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    Published online on: August 7, 2023
       https://doi.org/10.3892/etm.2023.12158
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Abstract

Proteus mirabilis is a common pathogen, which is responsible for urinary tract infections. Iron is a critical element necessary for both humans and pathogens to maintain their biological functions, and iron limitation via chelator agents may be useful in the treatment of infections. The present study aimed to investigate the synergistic interactions between the iron chelator agent deferoxamine (DFO) and the antibacterial drug colistin. The minimum inhibitory concentration (MIC) values of DFO and colistin for P. mirabilis isolates were determined by broth microdilution. The checkerboard technique was used to examine the potential synergy between DFO and colistin. Furthermore, time‑kill assays were used for the confirmation of synergy detected by the checkerboard assay, as well as for determining bacteriostatic and bactericidal interactions throughout a 24‑h period. As expected, all P. mirabilis isolates were resistant to colistin. DFO did not inhibit P. mirabilis growth when used alone, even at very high doses (10 µg ml‑1). Notably, when in combination with DFO, the MIC values of colistin were markedly reduced, and the checkerboard assay results showed synergy between colistin and DFO for all isolates. In addition, in time‑kill assays, colistin + DFO exhibited synergistic activity against all strains at most time intervals and concentrations tested. Colistin + DFO showed bactericidal activity at colistin concentrations of 1xMIC and 2xMIC, although a degree of re‑growth was observed in one of the strains at 12‑24 h. These findings indicated that DFO has the potential for use as an adjunct to colistin through iron sequestration, thus providing synergistic activity to an antibiotic that would not normally be considered a treatment option against P. mirabilis. In vivo experiments in the future may provide useful information on the efficacy of DFO/colistin since these models effectively reflect physiological parameters.
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Copy and paste a formatted citation
Spandidos Publications style
Erinmez M and Zer Y: Effects of deferoxamine on intrinsic colistin resistance of <em>Proteus mirabilis</em>. Exp Ther Med 26: 459, 2023.
APA
Erinmez, M., & Zer, Y. (2023). Effects of deferoxamine on intrinsic colistin resistance of <em>Proteus mirabilis</em>. Experimental and Therapeutic Medicine, 26, 459. https://doi.org/10.3892/etm.2023.12158
MLA
Erinmez, M., Zer, Y."Effects of deferoxamine on intrinsic colistin resistance of <em>Proteus mirabilis</em>". Experimental and Therapeutic Medicine 26.3 (2023): 459.
Chicago
Erinmez, M., Zer, Y."Effects of deferoxamine on intrinsic colistin resistance of <em>Proteus mirabilis</em>". Experimental and Therapeutic Medicine 26, no. 3 (2023): 459. https://doi.org/10.3892/etm.2023.12158
Copy and paste a formatted citation
x
Spandidos Publications style
Erinmez M and Zer Y: Effects of deferoxamine on intrinsic colistin resistance of <em>Proteus mirabilis</em>. Exp Ther Med 26: 459, 2023.
APA
Erinmez, M., & Zer, Y. (2023). Effects of deferoxamine on intrinsic colistin resistance of <em>Proteus mirabilis</em>. Experimental and Therapeutic Medicine, 26, 459. https://doi.org/10.3892/etm.2023.12158
MLA
Erinmez, M., Zer, Y."Effects of deferoxamine on intrinsic colistin resistance of <em>Proteus mirabilis</em>". Experimental and Therapeutic Medicine 26.3 (2023): 459.
Chicago
Erinmez, M., Zer, Y."Effects of deferoxamine on intrinsic colistin resistance of <em>Proteus mirabilis</em>". Experimental and Therapeutic Medicine 26, no. 3 (2023): 459. https://doi.org/10.3892/etm.2023.12158
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