Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p

Gu,Jia; Ye,Yongqing; Sunil,Rauniyar; Zhan,Wenjian; Yu,Rutong

doi:10.3892/etm.2023.12202

Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p

Authors:
- Jia Gu
- Yongqing Ye
- Rauniyar Sunil
- Wenjian Zhan
- Rutong Yu
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Affiliations: Department of Neurosurgery, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China, Institute of Nervous System Diseases, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China
Published online on: September 11, 2023 https://doi.org/10.3892/etm.2023.12202
Article Number: 503
Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The antisense transcript of SATB2 protein (SATB2‑AS1) is a novel long non‑coding RNA (lncRNA) which is involved in the development of colorectal cancer, breast cancer and hepatocellular carcinoma. In the present study, it was aimed to investigate the consequent situation of SATB2‑AS1 in tissue and cell lines of glioma. The expression of SATB2‑AS1 in glioma cases was analyzed in The Cancer Genome Atlas datasets. The glycolytic metabolism was determined in glioma cells by detection of extracellular glucose level, oxygen consumption rate and extracellular acidification rate. Cell Counting Kit‑8 assay and flow cytometry were used to assess cell proliferation and apoptosis in glioma cells. The interaction between SATB2‑AS1 and microRNA (miR)‑671‑5p was verified by bioinformatic analysis, reverse transcription‑quantitative PCR, dual luciferase reporter assay and RNA immunoprecipitation assay. The expression levels of the downstream targets of SATB2‑AS1 were studied by western blotting. Results demonstrated that SATB2‑AS1 was a downregulated lncRNA in low grade glioma and glioblastoma. Gain‑of‑function assay demonstrated that SATB2‑AS1 inhibited cell proliferation, and glycolytic metabolism, while induced cell apoptosis in glioma cells. SATB2‑AS1 sponged and suppressed the expression of an oncogenic miRNA miR‑671‑5p. By regulation of miR‑671‑5p, SATB2‑AS1 upregulated cerebellar degeneration related protein 1 (CDR1) and Visinin‑like 1 (VSNL1) expression in glioma cells. miR‑671‑5p overexpression partially reversed the antitumor effect of SATB2‑AS1 in glioma. In conclusion, the current study demonstrated that there was a downregulation of SATB2‑AS1 in glioma, and SATB2‑AS1 regulated miR‑671‑5p/CDR1 axis and miR‑671‑5p/VSNL1 axis in glioma.

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